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Assessment of bone mineral density by dual energy x-ray absorptiometry in patients with mucopolysaccharidoses
Orphanet Journal of Rare Diseases volume 8, Article number: 71 (2013)
Patients with mucopolysaccharidoses (MPS) are associated with poor bone growth and mineralization, however, information regarding the assessment of bone mineral density (BMD) in relation to age and treatment in this disorder is limited.
Dual energy x-ray absorptiometry (DXA) was performed in 30 patients with MPS (21 males and 9 females; 2 with MPS I, 12 with MPS II, 2 with MPS IIIB, 9 with MPS IVA, and 5 with MPS VI; median age, 10.8 years; age range, 5.0 years to 23.7 years; 26 patients were under 19 and 4 were above 19 years of age) to assess BMD of the lumbar spine (L1-L4), using the Hologic QDR 4500 system (Bedford, MA, USA).
For 26 patients under 19 years of age, standard deviation scores (z scores) for height, weight, body mass index (BMI), and BMD were −4.53 ± 2.66, -1.15 ± 1.55, 0.74 ± 1.23, and −3.03 ± 1.62, respectively, and they were all negatively correlated with age (p < 0.05). However, after correction for height-for-age z score (HAZ), HAZ adjusted BMD z score was −0.7 ± 1.24. Eight patients (31%) had osteopenia (HAZ adjusted BMD z score < −1 and ≥ −2), and 4 patients (15%) had osteoporosis (HAZ adjusted BMD z score < −2). Of 8 patients with MPS I, II or VI who underwent follow-up DXA after receiving enzyme replacement therapy for 1.0 to 7.4 years, all showed increase in absolute BMD values.
These findings and the follow-up data can be used to develop quality of care strategies for patients with MPS.
The mucopolysaccharidoses (MPS; OMIM 252700) are a group of inherited lysosomal storage disorders caused by deficiencies in enzymes catalyzing the degradation of glycosaminoglycans (GAGs). Progressive lysosomal accumulation of GAGs results in profound growth deficits, skeletal deformities (dysostosis multiplex), poor joint mobility, coarse facial features and organomegaly. Eleven known enzyme deficiencies give rise to 7 distinct types of MPS (I, II, III, IV, VI, VII, and IX). All are inherited in an autosomal recessive manner except for MPS II, which is an X-linked recessive disorder that occurs almost exclusively in males. Each MPS type exhibits a wide spectrum of clinical severity. Both severe and attenuated forms exist for MPS I (Hurler, Hurler-Scheie and Scheie syndromes) and MPS II (Hunter syndrome), and additional subtypes have been described for MPS III and MPS IV [1, 2]. The prevalence of MPS as a group is reported to be 1.9-4.5/100,000 live births with geographical differences in the frequencies of specific types. In Taiwan and other Asian countries, the most common type is MPS II, whereas in most Caucasian countries, it is MPS I or MPS III .
It is well-known that many children with chronic illnesses are at risk for low bone mass [4–9]. Patients with MPS have an increased risk of poor bone mineralization due to malnutrition, a particularly small frame, an abnormal gait, and reduction of physical activities caused by pain, poor health condition, or exercise intolerance . Although bone growth and mineralization have been reported to be affected by GAGs accumulation in animal models of MPS [11, 12], there is little published literature on the assessment of bone mineral density (BMD) in patients with MPS [10, 13, 14]. Dual energy x-ray absorptiometry (DXA) is the most frequently used tool to evaluate BMD because of its low radiation exposure and rapid scan time. Fung et al.  evaluated BMD by DXA in 8 patients with MPS II or VI who were receiving enzyme replacement therapy (ERT) and found that BMD was within the normal range for most of the patients, particularly after correction for short stature. However, BMD was not measured prior to the initiation of ERT, and thus, it is unclear whether the normal values existed prior to ERT or represented a treatment effect. Here, we characterized BMD and growth deficits in a group of 30 MPS patients not selected for skeletal problems and before the initiation of ERT. We hypothesized that MPS patients would have abnormally low BMD z scores, which has never been formally studied. Therefore we evaluated BMD by DXA in patients with MPS, in relation to age and treatment with ERT.
Materials and methods
Thirty patients with MPS (21 males and 9 females; median age, 10.8 years; age range, 5–23.7 years; 26 patients were under age 19 and 4 patients above age 19) were enrolled in this study at Mackay Memorial Hospital, Taipei, Taiwan. All patients underwent a history taking for skeletal problems, a physical examination to determine Tanner staging, and DXA. None had received ERT, hematopoietic stem cell transplantation or medications that might affect BMD prior to their baseline DXA. The diagnosis of MPS was confirmed by two-dimensional electrophoresis of urinary GAGs and enzyme activity assays in serum, leukocytes and/or skin fibroblasts. The study population consisted of 2 patients with MPS I (Hurler-Scheie), 12 with MPS II, 2 with MPS IIIB, 9 with MPS IVA, and 5 with MPS VI . Written informed consent was obtained from a parent for children and from patients over 18 years. The study was approved by the ethics committee of Mackay Memorial Hospital.
Each patient’s gender, age, MPS type, height, weight, and body mass index (BMI) were recorded at baseline. Puberty was defined as Tanner stage ≥3 by physical examination. A standard deviation score (z score) was derived by subtracting the population mean from each individual’s raw score and then dividing the difference by the standard deviation of the population. Z scores for height, weight, and BMI were calculated based on standard growth tables for Taiwanese children . Results are expressed as the mean ± standard deviation unless otherwise indicated. BMD of the lumbar spine (L1-L4) was assessed by DXA using the Hologic QDR 4500 system (Hologic, Bedford, MA, USA) . For the 26 patients under 19 years of age, the BMD results were converted to age- and gender-specific z scores based on the normative reference data for BMD in Taiwanese children . Due to the considerable height deficits for MPS patients, BMD z scores were then adjusted for height-for-age z score (HAZ) according to the method of Zemel et al.  which provides an adjustment for growth deficits in BMD by DXA. According to the classification used by our radiologists, osteopenia was defined as a HAZ adjusted BMD z score between −1 and −2 (<−1 and ≥ −2) and osteoporosis as a HAZ adjusted BMD z score < −2. For the 4 patients above age 19, the BMD results were converted to gender-specific T-scores based on normative reference data for BMD in the young adult Taiwanese population . The World Health Organization (WHO) defines osteoporosis in adults as a lumbar spine DXA T-score < −2.5.
The relationship between age and various physical characteristics of the 26 MPS patients under 19 years of age was determined using Pearson’s correlation coefficient (r), and significance was tested using Fisher’s r-z transformations. All statistical analyses were performed using SPSS version 11.5 (SPSS Inc., Chicago, IL, USA), and differences with p < 0.05 were considered statistically significant.
The 30 MPS patients (21 males, 9 females, all Asian) were evenly distributed by age from 5 years to early adulthood. The excess of males was attributable to the 12 male patients with MPS II, an X-linked recessive disorder. By medical history, there was a high prevalence of musculoskeletal disease, with 13 (43%) patients having skeletal pain, 30 (100%) joint stiffness, 20 (67%) kyphosis/gibbus, 14 (47%) scoliosis, 25 (83%) claw hands, 28 (93%) vertebral deformities, 14 (47%) vertebral instability, and 13 (43%) spinal cord compression.
Table 1 shows the mean z scores for height, weight, BMI, BMD, and HAZ-adjusted BMD by MPS type for the 26 patients <19 years of age. Similar results with T-scores are presented in Table 2 for the 4 patients ≥19 years of age. In patients <19 years of age, the mean z scores were below the normal range for height (−4.53 ± 2.66) and BMD (−3.03 ± 1.62), and within the normal range for weight (−1.15 ± 1.55) and BMI (0.74 ± 1.23). Correspondingly, most patients were below the normal range (<−2 z score) for height (81%) and BMD (69%), while only a few were below the normal range for weight (31%) and none for BMI. All four parameters were negatively correlated with age (r = −0.422 to −0.553, p <0.05) (Table 3 and Figures 1a-d). However, after adjusting for height, the mean HAZ-adjusted BMD z score increased into the normal range (−0.7 ± 1.24). Eight patients (31%) had osteopenia (HAZ-adjusted BMD z score < −1 and ≥ −2), and 4 patients (15%) had osteoporosis (HAZ adjusted BMD z score < −2). Unlike the previous parameters, there was no correlation between HAZ-adjusted BMD z score (Figure 1e).
In the 4 patients ≥19 years of age (Table 2), the pattern of characteristics was similar, but overall more severe than in the younger age group, with height (−8.39 ± 1.36 z score) and BMD (−4.88 ± 2.01 T-score) being relatively more affected than weight (−2.91 ± 0.43 z score) and BMI (−0.19 ± 0.97).
Eight patients with MPS I (n = 1), II (n = 3) and VI (n = 4) underwent follow-up DXA after receiving ERT for a mean of 4.3 ± 1.9 years (range 1.0 to 7.4 years), including 5 who were pre-pubertal and 3 post-pubertal at the start of ERT (Table 4). All 8 patients showed an increase in absolute BMD values, and 3 of the 5 patients who initiated ERT prior to puberty showed an improvement in HAZ-adjusted BMD z score (Table 4).
To the best of our knowledge, this is the largest single center study of patients with MPS evaluated for BMD using DXA prior to the initiation of ERT. We found that 46% (12/26) of the MPS study patients <19 years of age had HAZ adjusted BMD scores that met our criteria for osteopenia (z score ≥ −2 to < −1) or osteoporosis (z score < −2). The older patients had even lower z scores for height, weight and BMI, illustrating the progressive nature of the disease. DXA has been applied extensively to detect osteoporosis in postmenopausal women. BMD assessed by DXA is used to calculate a T-score (standard deviation score compared with the mean peak bone mass of normal young adults), which correlates with fracture risk in this population. With increasing awareness of osteoporosis in other populations, DXA has been extended for use in children. However, the interpretation of pediatric DXA results is complicated by ongoing bone growth and bone mineral accrual . Thus z scores based on age, gender, and race-specific reference data are required to categorize BMD values into normal or low density for chronological age (z score < −2). Furthermore, it is a major challenge to accurately interpret DXA results when accessing bone health in MPS patients, given their considerable height deficits, relatively thick skulls, and disproportionately large heads (macrocephaly). DXA-derived BMD is a 2-dimentional image or an “areal” (g/cm2) density rather than a true volumetric density (vBMD). Thus, smaller bones have lower BMD than larger bones, even with the same vBMD . As a result, for a child with severe short stature, like MPS, the BMD z score will probably be overdiagnosis of osteoporosis . Additionally, whole body BMD results will be overestimated if the skull bones are abnormally thickened and the head is relatively large. To reduce these estimation biases, we chose to measure BMD of the lumbar spine (L1-L4) and to calculate z scores using age, gender, and ethnicity-specific reference data , and adjusting for HAZ according to the method of Zemel et al.  for patients under 19 years of age.
In 2004, Rigante et al.  reported DXA-derived lumbar spine BMD for 3 patients with MPS III between 11 to 24 years of age. The BMD z scores of the 2 older non-weight bearing patients were lower than those of the youngest patient who could walk with support. Two years later in 2006, the same group reported normal lumbar spine BMD by DXA in 2 pre-pubertal children with MPS IV . The authors suggested that the skeletal defects probably resulted from nutritional deficiencies and an inability to walk, rather than from the genetic defect itself. However, the BMD z scores in these studies were not corrected for height deficits. Since our MPS study patients had significant height deficits (height z score = −4.53 ± 2.66), we compared the unadjusted and HAZ adjusted BMD z scores for these 26 subjects under 19 years of age (Figures 1d, 1e and 2). Before adjustment, 5 patients (19%) would have met the BMD criterion for osteopenia and 18 patients (69%) met the BMD criterion for osteoporosis. However, after correcting for HAZ, the number of patients with osteopenia increased to 8 (31%), while the number of patients with osteoporosis decreased to 4 (15%). For the 4 adult patients above 19 years of age, we provided only absolute BMD values and T-scores due to the unavailability of prediction equations for adult BMD z scores adjusted for HAZ (Tables 2 and 4). As with the aforementioned concerns, since the significant height deficits of these 4 adult patients (height z score = −8.39 ± 1.36) appeared to affect the T-scores, we did not interpret the results as indicative of osteopenia or osteoporosis.
The pathophysiologic basis for skeletal findings in the lysosomal storage disorders is not completely understood. In Gaucher disease, substantial infiltration of Gaucher cells in the bone marrow, osteosclerosis, and osteonecrosis all contribute to the clinical manifestations of bone pain, bone crises, osteopenia, pathologic fractures, and avascular necrosis of the femoral head [22, 23]. Similarly, in Fabry disease [24, 25], Pompe disease , and Niemann-Pick disease type B , osteopenia and osteoporosis have been reported as common skeletal manifestations. ERT is an effective treatment for the systemic manifestations of Gaucher disease, and it can have a significant impact on skeletal manifestations [26–28]. ERT also has been shown to alleviate morbidity and improve endurance in MPS I, II, and VI [29–34], but there is a paucity of literature describing the effects of ERT on BMD in MPS [10, 32]. Fung et al.  assessed vBMD in 6 patients with MPS VI before and after ERT. The authors found an inverse relationship between change in spine vBMD z score and change in height, suggesting that the BMD z score may decrease as a patient’s growth improves on ERT. Lin et al.  reported on an 18 year-old patient with MPS I whose BMD improved significantly after receiving ERT for 2 years. In our present study, all 8 patients with MPS I, II or VI who underwent follow-up DXA after receiving ERT for 1.0 to 7.4 years showed an increase in absolute BMD values, and 3 of 5 patients who started ERT before puberty demonstrated improvements in their HAZ-adjusted BMD z scores. Improvement of BMD in MPS patients receiving ERT is probably due to multiple mechanisms, such as reduced GAG storage in the bones, increased muscle strength and endurance, and improved pulmonary function and mobility [29–34]. Our findings support DXA as a clinically useful method for monitoring BMD in MPS patients and assessing its response to ERT. Further research, however, is needed to determine which of the multiple mechanisms responsible for increased BMD are most impacted by ERT. Besides ERT, patients with MPS may be candidates for investigative trials of adjunctive agents such as bisphosphonates .
Our study has several limitations. There were no healthy volunteers to serve as a control group. Instead, DXA results were expressed as absolute BMD values and transformed into BMD z scores based on normative reference data for Taiwanese children that account for age and gender [16, 18]. The HAZ adjusted BMD z scores that account for height were calculated for our study population using reference equations for a pediatric population from the United States that was taller (few or no subjects with height z scores < −2) and non-Black (presumably mostly Caucasian) . The effect of any racial differences is unknown but it is expected to be far outweighed by the major effect of height. Our study was limited to BMD of the lumbar spine, which may not be representative of other skeletal involvement. Finally, the age range of patients in our study was quite broad (5–23.7 years) and the number of patients was too small to draw any conclusions about differences between MPS types.
We found a high prevalence of osteopenia (31%) or osteoporosis (15%) by DXA in MPS patients under 19 years of age unselected for skeletal problems, and all 8 patients with MPS I, II, and VI showed an increase in absolute BMD values after receiving ERT for 1–7.4 years. DXA is a simple, accurate, and non-invasive method for measuring BMD, and we recommend screening and regularly monitoring of BMD by DXA for patients with MPS. Findings of severe bone mineral loss may identify MPS patients who are at increased risk for fracture-related morbidity and mortality and prompt appropriate counseling and intervention.
Neufeld EF, Muenzer J: The mucoplysaccharidoses. The Metabolic and Molecular Bases of Inherited Disease. Edited by: assoc, Scriver CR, Beaudet AL, Sly WS, Valle D, Childs B, Kinzler KW, Vogelstein B. 8th edition. New York: McGraw-Hill; 2001:3421-3452.
Chuang CK, Lin SP: Neurochemical changes and therapeutical approaches in mucopolysaccharidoses. Neurochemistry of Metabolic Diseases-Lysosomal storage diseases, phenylketouria and Canavan disease. Edited by: Sankar S, Michael A, Maheep B. Trivandrum, India: Transworld Research Network; 2007:1-20.
Lin HY, Lin SP, Chuang CK, Niu DM, Chen MR, Tsai FJ, et al: Incidence of the mucopolysaccharidoses in Taiwan, 1984–2004. Am J Med Genet A. 2009, 149A: 960-964. 10.1002/ajmg.a.32781.
van den Berg LE, Zandbergen AA, Van Capelle CI, De Vries JM, Hop WC, van den Hout JM, et al: Low bone mass in Pompe disease: muscular strength as a predictor of bone mineral density. Bone. 2010, 47: 643-649. 10.1016/j.bone.2010.06.021.
Wasserstein M, Godbold J, McGovern MM: Skeletal manifestations in pediatric and adult patients with Niemann Pick disease type B. J Inherit Metab Dis. 2013, 36: 123-127. 10.1007/s10545-012-9503-0.
Yilmaz K, Ozmen M, Bora Goksan S, Eskiyurt N: Bone mineral density in children with neurofibromatosis 1. Acta Paediatr. 2007, 96: 1220-1222. 10.1111/j.1651-2227.2007.00401.x.
Lin HY, Lin SP, Chuang CK, Chen MR, Chang CY, Niu DM: Clinical features of osteogenesis imperfecta in Taiwan. J Formos Med Assoc. 2009, 108: 570-576. 10.1016/S0929-6646(09)60375-2.
Kao CH, Chen CC, Wang SJ, Yeh SH: Bone mineral density in children with Down’s syndrome detected by dual photon absorptiometry. Nucl Med Commun. 1992, 13: 773-775.
Bembi B, Ciana G, Mengel E, Terk MR, Martini C, Wenstrup RJ: Bone complications in children with Gaucher disease. Br J Radiol. 2002, 75 (Suppl 1): A37-A44.
Fung EB, Johnson JA, Madden J, Kim T, Harmatz P: Bone density assessment in patients with mucopolysaccharidosis: A preliminary report from patients with MPS II and VI. J Pediatr Rehabil Med. 2010, 3: 13-23.
Rowan DJ, Tomatsu S, Grubb JH, Montaño AM, Sly WS: Assessment of bone dysplasia by micro-CT and glycosaminoglycan levels in mouse models for mucopolysaccharidosis type I, IIIA, IVA, and VII. J Inherit Metab Dis. 2013, 36: 235-246. 10.1007/s10545-012-9522-x.
Turner AS, Norrdin RW, Gaarde S, Connally HE, Thrall MA: Bone mineral density in feline mucopolysaccharidosis VI measured using dual-energy X-ray absorptiometry. Calcif Tissue Int. 1995, 57: 191-195. 10.1007/BF00310257.
Rigante D, Caradonna P: Secondary skeletal involvement in Sanfilippo syndrome. QJM. 2004, 97: 205-209. 10.1093/qjmed/hch041.
Rigante D, Buonuomo PS, Caradonna P: Early-onset osteoporosis with high bone turnover in children with Morquio-Brailsford syndrome. Rheumatol Int. 2006, 26: 1163-1164. 10.1007/s00296-006-0150-3.
Chuang CK, Lin SP, Chung SF: Diagnostic screening for mucopolysaccharidoses by the dimethylmethylene blue method and two dimensional electrophoresis. Zhonghua Yi Xue Za Zhi (Taipei). 2001, 64: 15-22.
Chen W, Chang MH: New growth charts for Taiwanese children and adolescents based on World Health Organization standards and health-related physical fitness. Pediatr Neonatol. 2010, 51: 69-79. 10.1016/S1875-9572(10)60014-9.
Lin HY, Lin SP, Chuang CK, Chen MR, Chang CY: Intravenous pamidronate therapy in Taiwanese patients with osteogenesis imperfecta. Pediatr Neonatol. 2008, 49: 161-165. 10.1016/S1875-9572(09)60002-4.
Shu SG: Bone mineral density and correlation factor analysis in normal Taiwanese children. Acta Paediatr Taiwan. 2007, 48: 323-327.
Zemel BS, Leonard MB, Kelly A, Lappe JM, Gilsanz V, Oberfield S, et al: Height adjustment in assessing dual energy x-ray absorptiometry measurements of bone mass and density in children. J Clin Endocrinol Metab. 2010, 95: 1265-1273. 10.1210/jc.2009-2057.
Yeh LR, Chen CK, Lai PH: Normal bone mineral density in anteroposterior, lateral spine and hip of Chinese men in Taiwan: effect of age change, body weight and height. J Chin Med Assoc. 2004, 67: 287-295.
Gafni RI, Baron J: Overdiagnosis of osteoporosis in children due to misinterpretation of dual-energy x-ray absorptiometry (DEXA). J Pediatr. 2004, 144: 253-257. 10.1016/j.jpeds.2003.08.054.
Binkovitz LA, Henwood MJ: Pediatric DXA: technique and interpretation. Pediatr Radiol. 2007, 37: 21-31. 10.1007/s00247-006-0153-y.
Wenstrup RJ, Roca-Espiau M, Weinreb NJ, Bembi B: Skeletal aspects of Gaucher disease: a review. Br J Radiol. 2002, 75 (Suppl 1): A2-A12.
Mersebach H, Johansson JO, Rasmussen AK, Bengtsson BA, Rosenberg K, Hasholt L, et al: Osteopenia: a common aspect of Fabry disease. Predictors of bone mineral density. Genet Med. 2007, 9: 812-818. 10.1097/GIM.0b013e31815cb197.
Germain DP, Benistan K, Boutouyrie P, Mutschler C: Osteopenia and osteoporosis: previously unrecognized symptoms of Fabry disease. Clin Genet. 2005, 68: 93-95.
Deegan PB, Pavlova E, Tindall J, Stein PE, Bearcroft P, Mehta A, et al: Osseous manifestations of adult Gaucher disease in the era of enzyme replacement therapy. Medicine (Baltimore). 2011, 90: 52-60. 10.1097/MD.0b013e3182057be4.
Goker-Alpan O: Therapeutic approaches to bone pathology in Gaucher disease: past, present and future. Mol Genet Metab. 2011, 104: 438-447. 10.1016/j.ymgme.2011.08.004.
Lin HY, Lin SP, Chuang CK, Wraith JE: Enzyme Replacement Therapy with Imiglucerase in a Taiwanese Child with Type I Gaucher Disease. J Chin Med Assoc. 2006, 69: 228-232. 10.1016/S1726-4901(09)70224-2.
Clarke LA, Wraith JE, Beck M, Kolodny EH, Pastores GM, Muenzer J, et al: Long-term efficacy and safety of laronidase in the treatment of mucopolysaccharidosis I. Pediatrics. 2009, 123: 229-240. 10.1542/peds.2007-3847.
Muenzer J, Beck M, Eng CM, Giugliani R, Harmatz P, Martin R, et al: Long-term, open-labeled extension study of idursulfase in the treatment of Hunter syndrome. Genet Med. 2011, 13: 95-101. 10.1097/GIM.0b013e3181fea459.
Hendriksz CJ, Giugliani R, Harmatz P, Lampe C, Martins AM, Pastores GM, et al: Design, baseline characteristics, and early findings of the MPS VI (mucopolysaccharidosis VI) Clinical Surveillance Program (CSP). J Inherit Metab Dis. 2013, 36: 373-384. 10.1007/s10545-011-9410-9.
Lin HY, Lin SP, Chuang CK, Chen MR, Chen BF, Wraith JE: Mucopolysaccharidosis under enzyme replacement therapy with laronidase-A mortality case with autopsy report. J Inherit Metab Dis. 2005, 28: 1146-1148. 10.1007/s10545-005-0211-x.
Lin HY, Chen MR, Chuang CK, Chen CP, Lin DS, Chien YH, et al: Enzyme replacement therapy for mucopolysaccharidosis VI-experience in Taiwan. J Inherit Metab Dis. 2011, 34: 241.
Lin SP, Shih SC, Chuang CK, Lee KS, Chen MR, Niu DM, et al: Characterization of pulmonary function impairments in patients with mucopolysaccharidoses-changes with age and treatment. Pediatr Pulmonol. 2013, 10.1002/ppul.22774.
This work was partially supported by a research grant MMH-101-111 from Mackay Memorial Hospital, Taipei, Taiwan. We would like to express our sincere thanks to Gerald F. Cox, M.D., Ph.D. (Genzyme, a Sanofi Company) for his critical review and valuable comments, and Ms. Tsai-Feng Ho for her professional assistance in biostatistics.
The authors declare that they have no competing interests.
HYL performed acquisition, statistical analysis and interpretation of data, and drafting of the manuscript. SPL participated in design of the study, interpretation of the data and helped to draft the manuscript. SCS and CKC performed biochemical analyses and revised the manuscript. MRC and DMN were responsible for patient screening. All authors read and accepted the manuscript.
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Lin, HY., Shih, SC., Chuang, CK. et al. Assessment of bone mineral density by dual energy x-ray absorptiometry in patients with mucopolysaccharidoses. Orphanet J Rare Dis 8, 71 (2013). https://doi.org/10.1186/1750-1172-8-71
- Bone mineral density
- Dual energy x-ray absorptiometry
- Enzyme replacement therapy