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Urine creatine metabolite panel as a screening test in neurodevelopmental disorders
Orphanet Journal of Rare Diseases volume 15, Article number: 339 (2020)
Cerebral creatine deficiency disorders (CCDD) are inherited metabolic disorders of creatine synthesis and transport. Urine creatine metabolite panel is helpful to identify these disorders.
We reviewed electronic patient charts for all patients that underwent urine creatine metabolite panel testing in the metabolic laboratory at our institution.
There were 498 tests conducted on 413 patients. Clinical, molecular genetics and neuroimaging features were available in 318 patients. Two new patients were diagnosed with creatine transporter deficiency: one female and one male, both had markedly elevated urine creatine. Urine creatine metabolite panel was also used as a monitoring test in our metabolic laboratory. Diagnostic yield of urine creatine metabolite panel was 0.67% (2/297). There were six known patients with creatine transporter deficiency. The prevalence of creatine transporter deficiency was 2.64% in our study in patients with neurodevelopmental disorders who underwent screening or monitoring of CCDS at our institution.
Even though the diagnostic yield of urine creatine metabolite panel is low, it can successfully detect CCDD patients, despite many neurodevelopmental disorders are not a result of CCDD. To the best of our knowledge, this study is the first Canadian study to report diagnostic yield of urine creatine metabolite panel for CCDD from a single center.
Cerebral creatine deficiency disorders (CCDD) are inherited metabolic disorders of creatine biosynthesis and transport. Two enzymes, arginine-glycine amidino transferase (AGAT) (EC 126.96.36.199) [encoded by GATM (MIM#602360)] and guanidinoacetate methyltransferase (GAMT) (EC#188.8.131.52) [encoded by GAMT (MIM#602360)] are required for the stepwise biosynthesis of creatine. Both enzyme deficiencies, AGAT (MIM#612718) and GAMT (MIM#612736) are inherited autosomal recessively. Circulating creatine is taken up by a sodium-dependent membrane bound creatine transporter (CRTR) [encoded by SLC6A8 (MIM#300036)] into high energy demanding tissues such as brain and muscle. CRTR deficiency (MIM#300352) results in an X-linked disorder. There have been < 120 patients with GAMT deficiency, < 20 patients with AGAT deficiency and < 200 patients with CRTR deficiency reported in the literature .
Clinical features of all three CCDD include global developmental delay, intellectual disability, seizures, movement disorder and behavioral problems, which are not specific to CCDD alone. However, the characteristic biochemical features of each CCDD is unique: elevated guanidinoacetate in body fluids in GAMT deficiency, low or low normal urine guanidinoacetate and low plasma guanidinoacetate in AGAT deficiency and elevated urine creatine in CRTR deficiency in males. Because of these abnormalities, a urine creatine metabolite panel that measures guanidinoacetate, creatine and creatinine can be used as a screening test for CCDD. As all three disorders impair creatine metabolism and transport, males and females with GAMT and AGAT deficiencies and males with CRTR deficiency have absent creatine in brain tissue when measured by magnetic resonance spectroscopy (MRS). Abnormal urine creatine metabolite panels and abnormal brain MRS guide the molecular genetic confirmation by direct sequencing of GAMT, GATM and SLC6A8. Females with a heterozygous likely pathogenic variant in SLC6A8 can have normal urine creatine or creatine peak in MRS . Identification of patients or individuals in the neonatal or early infantile period and initiation of the treatment results in a normal neurodevelopmental outcome in GAMT and AGAT deficiencies [2,3,4], which necessitates early identification of these disorders.
We performed a retrospective review of a urine creatine metabolite panel that measures guanidinoacetate, and creatine to determine (1) The diagnostic yield of the urine creatine metabolite panel when used as a screening for CCDD; (2) Prevalence of CCDD in the study population and at our institution; and (3) Diagnosis of secondary cerebral creatine deficiency disorders using the urine creatine metabolite panel.
Material and methods
The Hospital for Sick Children’s Research Ethics Board approved this study (Approval#1000063421). All individuals who underwent a urine creatine metabolite panel in our Metabolic Diseases Laboratory between January 2013 and February 2019 were included in our study. Urine samples were sent from pediatrics, developmental pediatrics, neurology, clinical genetics, and metabolic clinics at our institution or from outside of our institution for diagnostic investigations. Our Metabolic Diseases Laboratory is one of the clinical reference laboratories for the measurement of urine guanidinoacetate and creatine in Canada.
All measurements were performed at the time of send-out to the clinical biochemical laboratory as part of the clinical diagnostics. At the time of study start date (February 2019), we acquired all urine creatine metabolite panel results performed in the Metabolic Diseases Laboratory according to the methods and standard operating procedures of this laboratory. All results were interpreted by a clinical or biochemical geneticist, who are experts in the inconclusive range between the normal reference interval and clinically relevant abnormal measurements.
We entered the clinical features, biochemical investigations and neuroimaging results of all individuals who had medical records at our institution into an Excel Database.
Concentrations of urine guanidinoacetate, and creatine were determined using an underivatized liquid chromatography tandem mass spectrometry (LC–MS/MS) protocol using a previously reported method . Briefly, random urine collections from patients were aliquoted and stored at − 20 °C until testing. Once thawed and vortexed, 5 µl of patient sample was added to a diluent containing stable isotope internal standards for each analyte. Specimens were run in concert with calibrators and quality control materials on AB SCIEX API4000 QTrap and Waters Xevo TQ-S Mass Spectrometers using a Waters Atlantis column (dC18 150 × 3.0 mm, 3 µm). Comparison testing was performed at the time of the method development and validation and confirmed that there was no bias between the two instruments. Testing proficiency for creatine metabolite measurement was routinely assessed, meeting both internal (%CV < 10) and external quality program (ERNDIM) acceptance criteria. Creatinine was measured using the alkaline picrate method. Briefly, picric acid in an alkaline medium reacts with creatinine to form an orange coloured complex. Intensity of the coloured complex is measured by automated chemistry analyzer (Abbot Architect immunoassay analyzer), which is directly proportional to the concentration of creatinine in a urine sample. Guanidinoacetate, and creatine concentrations were normalized to creatinine.
Fisher’s exact test was used to compare clinical and neuroimaging features of CCD patients with patients with no molecular genetic diagnosis of CCD. Kruskal–Wallis rank sum test was used to identify if there were significant sex differences within each reference age group. All the statistical analysis was conducted using R software (version 1.2.1335). A p value of < 0.05 was considered to be statistically significant.
There were 498 urine samples for the measurement of urine creatine metabolite panel in 413 patients. Ninety-five patients, including 8 who were > 19 years old (age range was 19.5–57.5 years), were excluded as there was no clinical information provided. We had clinical, molecular genetics and neuroimaging features for 318 of the 413 patients with urine creatine panel metabolite measurements. Twenty-one out of 318 patients had urine creatine metabolite panel measurement for monitoring of creatine metabolism for their known inherited metabolic disorders including six patients with creatine transporter deficiency  and 15 patients with seven different inherited metabolic disorders (ornithine-δ-aminotransferase deficiency, ornithine transcarbamylase deficiency, citrullinemia type I, arginase deficiency, MELAS, MTHFR deficiency, MAT I/III deficiency) (Fig. 1 and 2, Additional file 1). We excluded those patients from our data analysis. The remaining 297 patients and their urine creatine panel metabolite measurements were analyzed. There were 112 females and 185 males. We depicted the number of patients with an abnormal urine guanidinoacetate and urine creatine in Fig. 1. We summarized the average, median, percentiles and their numbers for guanidinoacetate and creatine levels based on the age dependent reference ranges for males and females in Table 1. Urine guanidinoacetate (p = 0.001856) and creatine (p = 0.03946) levels were significantly different between males and females for the age group 0–60 months, whereas in other age groups, there was no statistically significant difference between males and females (Table 1). The average creatinine level was 6442 ± 5093.29 SD μmol/L (range 65–35,658).
Two patients had a new diagnosis of CRTR deficiency during the study period: one female with a next generation sequencing panel for intellectual disability and elevated urine creatine, and one male by whole exome sequencing (WES) and elevated urine creatine. We summarized both patients in Table 2.
Elevated guanidinoacetate was identified in 8.42% (25/297) of the patients and GAMT deficiency was excluded in 52% (13/25) of those patients either by normal brain MRS or by normal molecular genetic investigations or both (Table 3). We depicted elevated urine guanidinoacetate levels for 13 patients who had normal brain MRS, molecular genetic investigations or both in Additional file 2.
Low guanidinoacetate was identified in 2.02% (6/297) of the patients and AGAT deficiency was excluded in 50% (3/6) of those patients either by normal brain MRS or by normal molecular genetic investigations (Fig. 1 and Table 3). We depicted low urine guanidinoacetate levels for three patients who had normal brain MRS and molecular genetic investigations in Additional file 3.
Elevated urine creatine was identified in 24% (72/297) of the patients and CRTR deficiency was excluded in 53% (38/72) of males and females either by normal brain MRS or by normal molecular genetic investigations or both (Fig. 1 and Table 4). We depicted the number of elevated urine creatine and the range of elevations in patients who had normal brain MRS, and/or normal molecular genetic investigations in Additional file 4. Two patients with CRTR deficiency diagnosed during the study period were summarized above.
In the remaining 295 patients with no CCDD, all patients had neurodevelopmental disorders. Seizures were present in 42% of the patients. Behavioral disorders reported in 26% of the patients. Movement disorders were present in 17% of the patients and ataxia was the most common movement disorder.
We summarized the clinical features, neuroimaging and molecular genetic investigations of 58 patients with genetic diagnoses and CRTR deficiency in Additional file 1. Thirty-seven of these patients underwent a urine creatine metabolite panel for diagnostic investigations of neurodevelopmental disorders and 21 of these patients had a urine creatine metabolite panel for monitoring of creatine metabolism due to their known inherited metabolic disorder. We depicted 35 different genetic diagnoses (except CRTR deficiency) and the number of patients for each in Fig. 2. The most common genetic disease was ornithine aminotransferase deficiency in our study cohort. During the monitoring of creatine metabolism in known inherited metabolic disorders, we found abnormal urine guanidinoacetate and/or urine creatine in several patients. Results of all of these patients are listed in Additional file 1.
In our retrospective cohort study, we report a diagnostic yield of 0.67% (2/297) for the urine creatine metabolite panel if it would have been used as a first-line screening tool during the study period of 6 years. Two patients that identified with CRTR deficiency during the study period had non-targeted molecular genetic investigations: one next generation sequencing panel for intellectual disability and one WES as a first-line investigation, which led to the biochemical confirmation of CRTR deficiency. Interestingly, the female patient had a markedly elevated urine creatine. There were 6 additional patients with CRTR deficiency who underwent the urine creatine metabolite panel for monitoring. Altogether, the prevalence of creatine transporter deficiency was 2.64% (8/303) in our study cohort of patients with neurodevelopmental disorders who underwent screening or monitoring of CCDS at our institution.
In our study, the diagnostic yield of WES was 27% (14/52) in patients with neurodevelopmental disorders [CRTR deficiency (n = 1); ARX disease (n = 1); KDM5C disease (n = 1); GABRB3 disease (n = 1); KIAA2022 disease (n = 1); KCNA2 disease (n = 1); PMM2-CDG (n = 2); SYNGAP1 disease (n = 1); Angelman syndrome (n = 1); PURA disease (n = 1); EARS disease (n = 1); HECW2 disease (n = 1); KAT6B disease (n = 1)]. Interestingly, the diagnostic yield of WES for CRTR deficiency was 1.9% (1/52) in our study. The diagnostic yield of WES seems to be higher than the urine creatine metabolite panel in the diagnosis of CRTR deficiency (2/52 vs 2/297).
Previously, we reported a 1–30-fold elevation of urine guanidinoacetate levels in molecular genetically confirmed patients with GAMT deficiency . In our current study, urine guanidinoacetate levels were elevated up to 4.4-fold above the upper reference range in 25 patients. We excluded GAMT deficiency based on the normal brain MRS and/or molecular genetic investigations in 52% of those patients. We did not have any true positive GAMT deficiencies in our study cohort, and we are not certain if elevated urine guanidinoacetate had good sensitivity. GAMT deficiency is unlikely to be missed by urine creatine metabolite panel, but a positive test needs to be investigated further. We have two true positive CRTR deficiencies in our study cohort; markedly elevated urine creatine is likely highly sensitive. CRTR deficiency is not missed by urine creatine metabolite panel, but a positive test needs to be investigated further.
Secondary cerebral creatine deficiency was previously reported in argininosuccinate lyase, argininosuccinate synthetase, ornithine aminotransferase and Δ-pyrroline-5-carboxylate synthetase deficiencies [8,9,10]. Urine guanidinoacetate measurements were helpful to identify some of the secondary creatine deficiency disorders including ornithine-δ-aminotransferase deficiency and ornithine transcarbamylase deficiency in our study. In ornithine-δ-aminotransferase deficiency, low creatine levels are due to two mechanisms: (1) elevated plasma ornithine inhibits AGAT enzyme and decreases guanidinoacetate synthesis; (2) high ornithine competes with arginine to decrease substrate supply to creatine synthesis. In proximal urea cycle disorders, arginine deficiency results in a lack of arginine supply to creatine biosynthesis and decreased guanidinoacetate and creatine synthesis. Low urine guanidinoacetate levels will likely suggest not only AGAT deficiency, but also ornithine-δ-aminotransferase deficiency, proximal urea cycle disorders, as well as failure to thrive and decreased protein intake .
In our recent study, males with molecular genetically confirmed CRTR deficiency had 1–12-fold elevation of urine creatine . In our current study, some of the patients had up to a fivefold elevation of urine creatine with normal brain MRS or normal molecular genetic investigations excluding the CRTR deficiency. It seems that there is some overlap between true CRTR deficiency and false positive elevation of creatine.
It should be noted that our study had several limitations including: (1) It is a retrospective cohort study consisting of a chart review of patients that underwent a urine creatine metabolite panel for screening of CCDD at our institution; (2) Despite abnormal urine creatine metabolite panel results being identified in 103 patients, there were no brain MRS or molecular genetic investigations in 49 of those patients. We are not certain if those patients with an abnormal urine creatine metabolite panel might have had CCDD; (3) Due to the lack of confirmatory investigations in 49 patients, we were not able to estimate the true prevalence or the false positive rate of CCDD using the urine creatine metabolite panel or update our reference range to decrease the false positive rate for CCDD; (4) Due to lack of confirmatory investigations in 49 patients, we were not able to calculate the sensitivity and specificity of the urine creatine metabolite panel; (5) In our recent study, WES was applied to 109 children with epilepsy and none of the patients had CCDD . Whereas in our current study, the diagnostic yield of WES was 1.9% for CRTR deficiency, which is unlikely to be a true prevalence of CRTR deficiency in patients with neurodevelopmental disorders who underwent WES; 6) Two of the study authors (S.M-A and A.S.) receive referrals for all patients with CCDD for their management. In more than a 10 years’ time period, there were only 8 patients (including the two patients identified during this study period) known to the authors at our institution. To the best of our knowledge, those are the only patients identified either by a urine creatine metabolite panel, by brain MRS or by molecular genetic investigations over the period of more than 10 years.
In conclusion, we report a low diagnostic yield of the urine creatine panel in patients with neurodevelopmental disorders. Although the diagnostic yield of the urine creatine metabolite panel as a screening tool for neurodevelopmental disorders is low, the low cost and non-invasive nature make urine testing a suitable first-tier test in neurodevelopmental disorders. To the best of our knowledge, this study is the first Canadian study to report on the diagnostic yield of the urine creatine panel for CCDD from a single center. Despite several limitations, our study results are valuable in evaluating the clinical use of a urine creatine panel to identify treatable CCDD.
Availability of data and materials
The authors confirm that the part of the data supporting the findings of this study are available within the article [and/or] its supplementary material based on the Research Ethics Board approval. The data that support the findings of this study analysis are available on request from the corresponding author. The data are not publicly available due to the data containing research participants’ information which could compromise the privacy of the participants.
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We would like to thank our patient’s parents for allowing us to present their child’s results.
We would like to thank The Division of Clinical and Metabolic Genetics Starbucks Clinical Genetics/Genomics Research Studentship Award and Centre for Genetics/Genomic Research Foundation for their funding support. We would like to thank the physicians in the Divisions of Clinical and Metabolic Genetics and Neurology for providing excellent clinical care to their patients. We would like to thank the Metabolic Laboratory technicians for their dedication and excellent work for providing clinical metabolic diagnostic investigations.
This study is funded by the Division of Clinical and Metabolic Genetics and Centre for Genetics/Genomic Research Studentship Award.
Ethics approval and consent to participate
The Hospital for Sick Children Research Ethics Board (Approval# 1000063421) approved this study. All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2000. The study was approved without informed consent as a retrospective chart review study. There is no identifying patient information included in the article.
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The authors declare that they have no competing interests.
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Demographics, clinical features, urine creatine panel, neuroimaging and molecular genetic test results of patients who have undergone urine creatine panel and diagnosed with other genetic diagnoses are listed in Supplemental Table 1.
Elevated urine guanidinoacetate levels in patients, who had normal brain MRS or molecular genetic investigations or both investigations, are depicted in Supplemental Figure 1. Red line shows upper limit of reference range.
Low urine guanidinoacetate levels in patients, who had normal brain MRS or molecular genetic investigations or both investigations, are depicted in Supplemental Figure 2. Red line shows lowest limit of reference range.
Elevated urine creatine in patients, who had normal brain MRS or molecular genetic investigations or both investigations, are depicted in Supplemental Figure 3. Red line shows upper limit of reference range.
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Bahl, S., Cordeiro, D., MacNeil, L. et al. Urine creatine metabolite panel as a screening test in neurodevelopmental disorders. Orphanet J Rare Dis 15, 339 (2020). https://doi.org/10.1186/s13023-020-01617-z
- Cerebral creatine deficiency syndromes
- Global developmental delay