Orphanet Journal of Rare Diseases volume 1, Article number: 3 (2006)
Celiac disease is a chronic intestinal disease caused by intolerance to gluten. It is characterized by immune-mediated enteropathy, associated with maldigestion and malabsorption of most nutrients and vitamins. In predisposed individuals, the ingestion of gluten-containing food such as wheat and rye induces a flat jejunal mucosa with infiltration of lymphocytes. The main symptoms are: stomach pain, gas, and bloating, diarrhea, weight loss, anemia, edema, bone or joint pain. Prevalence for clinically overt celiac disease varies from 1:270 in Finland to 1:5000 in North America. Since celiac disease can be asymptomatic, most subjects are not diagnosed or they can present with atypical symptoms. Furthermore, severe inflammation of the small bowel can be present without any gastrointestinal symptoms. The diagnosis should be made early since celiac disease causes growth retardation in untreated children and atypical symptoms like infertility or neurological symptoms. Diagnosis requires endoscopy with jejunal biopsy. In addition, tissue-transglutaminase antibodies are important to confirm the diagnosis since there are other diseases which can mimic celiac disease. The exact cause of celiac disease is unknown but is thought to be primarily immune mediated (tissue-transglutaminase autoantigen); often the disease is inherited. Management consists in life long withdrawal of dietary gluten, which leads to significant clinical and histological improvement. However, complete normalization of histology can take years.
Disease name and synonyms
Celiac disease (CD) in children and celiac sprue in adults are probably the same disorder with the same pathogenesis. The synonyms are: Coeliac disease (British spelling) – Celiac sprue – Nontropical sprue-Gluten-sensitive enteropathy – Idiopathic steatorrhea
Celiac disease is a chronic intestinal disease mostly associated with malabsorption caused by intolerance to gluten. It is characterized by immune-mediated enteropathy (villous flattening), resulting in maldigestion and malabsorption. Clinical and histological improvement can be obtained after withdrawal of dietary gluten.
Celiac disease is characterized by malabsorption and villous atrophy. However, diseases other than CD can cause marked villous flattening and increased intraepithelial lymphocytes (IEL) . Differential diagnosis is of special importance for subjects in whom CD is suspected and who have negative serology. The following diseases, which can have similar features, must be ruled out [1–4]:
Crohn's disease of the small intestine
Small intestinal lymphoma
Carbohydrate intolerance, cow's milk intolerance
Prevalence of clinically overt celiac disease varies from 1/270 in Finland to 1/5,000 in North America. However, since celiac disease can be asymptomatic, most subjects are not diagnosed or they can present with atypical symptoms. In epidemiological studies aimed to assess CD prevalence, large cohorts in North America and Europe were screened for highly-sensitive endomysium or tissue transglutaminase antibodies. Besides, they underwent subsequent small intestinal biopsies when antibody testing was positive. The CD prevalence was found to be much higher than expected. Approximately 1/100 to 1/500 were found positive for antibodies and had villous atrophy of the small intestine [5–10]. Thus, up to 1% of a western population tests positive for celiac disease. There are approximately 7–10 undiagnosed subjects for each known CD patient. Furthermore, approximately 10% of the first-degree relatives also have CD [11, 12].
Celiac disease is diagnosed typically in early childhood around age of 2 years. A second peak is found around age of 40 years . Most symptoms are due to malabsorption of nutrients and vitamins [13, 14]. However, the clinical manifestations differ greatly, depending on each case and ranging from asymptomatic (silent) to full blown (symptomatic, clinically overt) celiac disease. The severity of symptoms is not necessarily proportional to the severity of the mucosal lesions and patients with total villous atrophy can be asymptomatic or present with subclinical symptoms such as iron deficiency or muscle cramps. Nowadays, more subjects present with asymptomatic or mild celiac disease than with the classical symptoms of severe malabsorption [4, 17].
The term "atypical" celiac disease is used for patients who present with extraintestinal symptoms like Immunoglobulin A (IgA)-nephropathy, hemosiderosis of the lungs and a variety of neurological diseases. Antibodies and typical small intestinal changes can be found. Early diagnosis is desirable since many of these symptoms can disappear after the initiation of a gluten-free diet.
The term "latent" celiac disease refers to subjects who will develop the disease later in life but who do not have a flat mucosa despite a gluten-containing diet [17–20]. Increased intraepithelial lymphocytes (IEL) and positive endomysium antibody (EMA) or positive tissue transglutaminase (tTG) antibody tests are sometimes found in these subjects [21–23]. What triggers the onset of the disease in these subjects remains unknown.
Ferguson et al. introduced the term "potential" celiac disease in 1993 to characterize in details patients with latent CD . The authors suggested "potential" CD to be used for the subjects who have markers of latent CD (elevated IEL, positive for tTG) without ever developing overt CD, with "latent" CD being used for patients who will develop a flat mucosa in the future. However, this discrimination is very artificial and not shared by other specialists in the field . The terms "latent" and "potential" celiac disease are not used by all authors in the same way, which can further confuse matters. Patients with latent or potential celiac disease may develop symptoms that respond to a gluten-free diet . For the definition of the different states of celiac disease, see table 1.
Celiac disease is also associated with several extraintestinal diseases and autoimmune diseases, which can not be linked to nutrient deficiencies [27–33]. For example, up to 8% of patients with type 1 diabetes were reported to test positive for CD . CD patients are also at higher risk of developing malignancies. Holmes et al. reported an increased risk especially of intestinal lymphoma in subjects with untreated celiac disease compared to patients on a gluten-free diet . However, more recent data indicate that this risk may be lower than previously anticipated [35, 36].
Peripheral neuropathy (vitamin B12 and B1 deficiency)
Anemia (iron, vitamin B12 and folate deficiency)
Growth failure in children
Bone pain (osteoporosis and osteopenia, vitamin D and calcium deficiency)
Muscle cramps (magnesium and calcium deficiency)
Night blindness (vitamin A deficiency)
Weight loss (impaired absorption of most nutrients)
Edema (protein and albumin loss)
Weakness (hypokalemia and electrolyte depletion)
Bleeding and hematoma (vitamin K deficiency)
The following extraintestinal symptoms/manifestations are probably not secondary to malabsorption (atypical CD) 
Neurological disorders such as depression, epilepsy, migraine, ataxia
Elevated liver enzymes, liver failure
Idiopathic pulmonary hemosiderosis
Autoimmune diseases such as type 1 diabetes, Sjögren syndrome, thyroid diseases (Hashimoto's thyroiditis and Graves's disease), autoimmune hepatitis and primary biliary cirrhosis
Selective IgA deficiency
Firm diagnosis of CD can only be established after small intestinal biopsies confirming a flat jejunal mucosa with absence of normal intestinal villi [2, 3]. Histological examination further demonstrates a cellular infiltrate of lamina propria consisting of plasma cells and lymphocytes . The number of intraepithelial lymphocytes and especially the number of gamma/delta T cells is markedly increased (>40 IEL/100 epithelial cells) [38, 39]. Small intestinal changes can vary from a nearly normal mucosa with increased IEL  to a completely flat mucosa . Pathologists write a standardized report to characterize the histological features of celiac disease  (see table 2). In cases with minor histological changes, a six-week gluten challenge and subsequent endoscopy can be performed. In subjects with negative serology (see below), diseases other than CD that can cause villous flattening must be ruled out . Thus, the diagnosis of celiac disease should not depend only on biopsy, but also the clinical picture and serology should be considered.
Antibody tests cannot replace histology but are very helpful as a screening tool in asymptomatic subjects at higher risk of developing celiac disease (first-degree relatives and patients with autoimmune diseases, e.g. diabetes) . Until recently, the determination of IgA endomysium antibodies was the most important laboratory test in the diagnosis of CD [3, 44]. In some scientific research institutions this test reaches a sensitivity and specificity around 97%. However, in routine laboratories, this test is much less sensitive (giving rise to more false-negative results) [45, 46]. Since frozen sections of monkey esophagus are used for this assay, it is very expensive. The autoantigen, which is recognized by endomysium antibodies (EMA), is now discovered and shown to be tissue transglutaminase (tTG). Subsequently, several ELISA tests for detection of tTG antibodies were developed. They have the same sensitivity and specificity as EMA assays [47–49]. Occasionally, tTG antibodies may detect CD patients undiagnosed by endomysial antibodies and vice versa . These new tests are cheaper and the results obtained are much better reproducible. The first generation of tests used guinea pig tTG. They were less sensitive and specific  than the new tests that use human transglutaminase [52–54]. However, the quality of the different tTG-test kits can also differ markedly . Consequently, strong false positive tTG results were reported in the clinical practice .
For routine diagnosis, the determination of gliadin antibodies are no longer required [56, 57]. They are less sensitive and specific than EMA and tTG antibody tests. The sensitivity of IgA gliadin antibodies is around 80–90% and the specificity around 85–95%. IgG gliadin antibodies are even less sensitive (75–85%) and specific (75–90%). However, they allow the detection of patients with both celiac disease and IgA deficiency. Patients with IgA deficiency may have negative IgA-gliadin, EMA and tTG tests. IgA-tTG in combination with IgG-tTG antibody assays (to detect subjects with IgA deficiency) have frequently replaced all other tests [58–63].
Seroconversion of tTG antibodies after the initiation of a gluten-free diet is not necessarily accompanied with morphological recovery of the mucosa. After one year of a gluten-free diet a substantial number of celiac patients turned negative for tissue transglutaminase or endomysial antibodies but still manifested villous atrophy [64, 65]. The normalization of the mucosa can take several years . On the other hand, some patients might have positive tissue transglutaminase antibodies but a completely normal mucosa. Thus, after the initiation of a gluten-free diet, antibody tests are not very helpful to draw a conclusion about the condition of the mucosa. The determination of IgA-tTG antibodies might be helpful to monitor the success of the gluten-free diet . However, others reported that their negativity is a falsely secure marker of strict diet compliance .
Management including treatment
Two guidelines about the management of CD were recently published: Recommendations of the North American Society for Pediatric Gastroenterology, Hepatology and Nutrition  and National Institutes of Health (NIH) consensus development conference statement on celiac disease . Once the diagnosis of celiac disease has been firmly established (see diagnostic methods), gluten has to be immediately withdrawn. Dietary gluten is present mainly in wheat, rye and barley [2, 41, 69]. Since small amounts of gluten are hidden in many food products, dietary counseling is absolutely necessary. In most countries, support groups for celiac disease greatly help patients by providing them with adequate dietary information.
Gluten is also found in oats, however many studies suggested that the ingestion of oats is safe for most patients [70–74]. This data was in line with recent studies, which failed to identify the toxic amino acid sequences in oats (see below) . Despite these observations, the ingestion of oats can not be endorsed, since commercial oats are often contaminated with wheat or rye. In addition, the oats-containing gluten-free diet caused in some individuals more intestinal symptoms than the traditional diet. Rarely, mucosal integrity was disturbed and more inflammation was evident in the group on oats diet. Nevertheless, oats may provide an alternative in the gluten-free diet; at the same time, patients should be aware that the intestinal symptoms may worsen [76, 77]. Antibodies to oat prolamines were more frequently found higher in children with CD ; however, the significance of this finding is not clear.
According to the European Society of Pediatric Gastroenterology and Nutrition (ESPGAN) criteria, repeated endoscopy with jejunal biopsy is not necessary if the patient's condition improves after introducing a gluten-free diet . The results of repeated endoscopy could be rather confusing since normalization of the histology may take up to eight years . At the same time, persistent mucosal abnormalities were described despite a strict gluten-free diet [79, 80]. Thus, there is no point in repeating endoscopy when the patient improves on a gluten-free diet. Vitamin supplementation may be necessary at the beginning of a gluten-free diet in subjects with severe celiac disease. Patients with clinically overt CD should go on a strict gluten-free diet since those not treated are at a higher risk of malignancies , anemia and osteoporosis [13, 17, 81]. In addition, the onset of autoimmune diseases which are associated with celiac disease seem to be related to the duration of exposure to gluten . However, this issue is controversial especially in adults: negative reports have been published in Italy and Finland [83, 84].
In subjects who do not respond to a gluten-free diet, non compliance or inadvertent ingestion of gluten should be considered . Microscopic colitis in patients with persistent diarrhea should be ruled out by colonoscopy . Small intestinal bacterial overgrowth was reported to be frequently present in CD . Patients with this condition can be identified by a hydrogen breath tests (H2 breath test). They rapidly improve after the initiation of an antibiotic regimen. When these conditions are ruled out, other diseases such as intestinal lymphoma  or refractory sprue should be envisaged .
Whether asymptomatic screen-detected patients (with normal laboratory values and no gastrointestinal symptoms) should adhere to a gluten-free diet remains a controversial issue [90, 91]. Some authors suggest the silent cases of CD to be treated with a life long gluten-free diet, otherwise they are exposed to the risks of long-term complications. On the other hand, 1) until now, no study has proven the benefit of a gluten-free diet for this subgroup of patients; 2) the compliance of these subjects to follow a gluten-free diet is known to be very low; 3) the relative risks for lymphomas and gastrointestinal cancers in patients with CD was found lower than previously thought [35, 92, 93] with no elevated cancer risk during childhood and adolescence [35, 36].
Celiac disease develops in patients who have ingested gluten, which is present in wheat, rye and barley (recent reviews: [2–4]). Gluten proteins are grouped into high molecular weight (HMW) glutenins, low molecular weight (LMW) glutenins and alpha-, gamma- and omega-gliadins (based on their differential N-terminal sequence, electrophoresis size and mobility) . Until recently it was thought that the toxic proteins causing CD are not present in the HMW glutenins. In consequence, development of non-toxic HMW glutenins-containing food products was proposed (e.g. transgenic food). However, recent evidence showed that there is no group of gluten proteins safe for patients with CD . Interestingly, gluten extracted from several ancient wheat species was reported incapable to stimulate T cell lines (previously shown to be responsive to toxic gliadin fragments) (see below) . These findings raise the prospect of identifying bred wheat species with low or absent levels of harmful gluten proteins.
Based on the high association between human leukocyte antigens (HLA) and celiac disease (over 95%) , it is thought that HLA-DQ2 positive antigen-presenting cells have gliadin peptides toxic to CD4+ T cells. CD4+ T cells drive the immune response and damage the mucosa [97–102]. Tissue transglutaminase (tTG) was identified as the autoantigen, which is recognized by the endomysial antibodies [57, 103] (see below).
Tissue transglutaminase, an enzyme essential in wound healing for all individuals, was shown to be able to deamidate gliadin peptides in vitro [98–101]. The modified gliadin peptides bind much better to HLA-DQ2 and elicit a stronger T cell response. Furthermore, gliadin is one of the main substrates of tTG. It was reported that several peptides can be cross-linked and that tTG itself can be incorporated into HMW complexes. These newly generated molecules are potential "neo"-autoantigens, which might be responsible for the induction of a destructive immune response.
Gliadin-reactive CD4+ T cells were isolated from the intestinal mucosa of patients with celiac disease . These T cells can elicit a B cell response with production of autoreactive antibodies against gliadin peptides or tTG. However, whether the role of these antibodies is significant  or whether they have a pathogenic role in celiac disease remains unknown . It also remains unknown why tTG or gliadin complexes are recognized only by T cells from celiac patients and not by those from healthy HLA-DQ2 positive subjects. In addition, the increase in CD8+ T cells in the epithelium of the mucosa (IEL) cannot be explained by HLA-DQ2 positive cells, which only activate CD4+ T cells. Additional factors like infections, which damage the mucosa may trigger the onset of the disease in predisposed individuals.
In recent years, considerable progress has been made in identifying the amino acid sequences of gliadin peptides that may trigger CD. From these studies it became evident that there is not just one peptide but many gliadin peptides, which are capable to stimulate T cell lines from patients with CD [106–109]. All toxic gliadin fragments had a high content of the amino acid proline [75, 106, 107]. A "super" gliadin 33 amino acids peptide, capable to stimulate all gliadin specific T cell lines in a very vigorous manner, has been identified by the group of Shan L et al. . This gliadin fragment is rich in proline and contains multiple short amino acid sequences, which were previously shown to stimulate T cell lines [106, 111–113]. In addition, this peptide was completely resistant to the breakdown of endogenous proteases and peptidases, indicating that the full length fragment can reach the small intestine and stimulate mucosal T cells. The same research group described a bacterial prolyl endopeptidase (from Flavobacterium meningosepticum), which is capable to digest in vitro all proline rich peptides, including the 33 amino acid long "super" gliadin fragment . The authors suggested that patients with celiac disease might be able to tolerate gluten by supplementing the food with this enzyme. Currently, this hypothesis is under investigation (see Celiac Sprue Research Foundation ; Gluten detoxification trial). However, it is unlikely that all toxic gluten peptides would be efficiently destroyed, so this enzyme treatment would fail to prevent the gluten toxicity completely [108, 116].
Celiac disease is associated with HLA-DQ2/DQ8 in over 95% of the cases . However, 20% of the healthy population carry the same gene and will never develop celiac disease. Thus, no genetic test which could identify "celiac genes" is currently available. Furthermore, there is no need for genetic screening since celiac disease can be treated by a specific diet and patients usually enjoy a good quality of life and a normal life expectancy. In subjects with uncertain diagnosis of CD, the determination of the HLA genes might be helpful . HLA-DQ2/DQ8 negative subjects are highly unlikely to suffer from CD.
Celiac disease is the only autoimmune disease in which the agents that trigger the disease are identified, i.e. gliadin, the autoantigen transglutaminase and even the HLA-genes (DQ2/DQ8) which are associated with the disease. However, the exact mechanisms damaging the intestinal mucosa and the exact role of autoantibodies such as tTG and EMA antibodies in the pathogenesis of the disease remains unsolved. Furthermore, the question of how much gluten is toxic (e.g. trace amounts of contaminated gluten) is also a matter of discussion in both Europe and USA.
Goldstein NS: Non-gluten sensitivity-related small bowel villous flattening with increased intraepithelial lymphocytes: not all that flattens is celiac sprue. Am J Clin Pathol. 2004, 121: 546-550. 10.1309/10FC-NCTC-56NM-N0YE.
Ciclitira PJ: AGA technical review on celiac sprue. Gastroenterology. 2001, 120: 1526-1540.
Farrell RJ, Kelly CP: Celiac sprue. N Engl J Med. 2002, 346: 180-198. 10.1056/NEJMra010852.
Abdulkarim AS, Murray JA: The diagnosis of coeliac disease. Aliment Pharmacol Ther. 2003, 17: 987-995. 10.1046/j.1365-2036.2003.01442.x.
Not T, Horvath K, Hill ID, Partanen J, Hammed A, Magazzu G, Fasano A: Celiac disease risk in the USA: high prevalence of antiendomysium antibodies in healthy blood donors. Scand J Gastroenterol. 1998, 33: 494-498. 10.1080/00365529850172052.
Catassi C, Ratsch IM, Fabiani E, Rossini M, Bordicchia F, Candela F, Coppa GV, Giorgi PL: Coeliac disease in the year 2000: exploring the iceberg. Lancet. 1994, 343: 200-203. 10.1016/S0140-6736(94)90989-X.
Maki M, Mustalahti K, Kokkonen J, Kulmala P, Haapalahti M, Karttunen T, Ilonen J, Laurila K, Dahlbom I, Hansson T, Hopfl P, Knip M: Prevalence of Celiac disease among children in Finland. N Engl J Med. 2003, 348: 2517-2524. 10.1056/NEJMoa021687.
McLoughlin R, Sebastian SS, Qasim A, McNamara D, O'Connor HJ, Buckley M, O'Morain C: Coeliac disease in Europe. Aliment Pharmacol Ther. 2003, 18: 45-48. 10.1046/j.1365-2036.18.s3.1.x.
Dube C, Rostom A, Sy R, Cranney A, Saloojee N, Garritty C, Sampson M, Zhang L, Yazdi F, Mamaladze V, Pan I, MacNeil J, Mack D, Patel D, Moher D: The prevalence of celiac disease in average-risk and at-risk Western European populations: A systematic review. Gastroenterology. 2005, 128: S57-S67. 10.1053/j.gastro.2005.02.014.
Collin P, Reunala T, Rasmussen M, Kyronpalo S, Pehkonen E, Laippala P, Maki M: High incidence and prevalence of adult coeliac disease. Augmented diagnostic approach. Scand J Gastroenterol. 1997, 32: 1129-1133.
MacDonald WC, Dobbins WO, Rubin CE: Studies on the familial nature of celiac sprue using biopsy of the small intestine. N Engl J Med. 1968, 272: 448-456.
Auricchio S, Mazzacca G, Tosi R, Visakorpi J, Maki M, Polanco I: Coeliac disease as a familial condition: Identification of asymptomatic coeliac patients within family groups. Gastroenterology Intl. 1988, 1: 25-31.
Kemppainen T, Kroger H, Janatuinen E, Arnala I, Kosma VM, Pikkarainen P, Julkunen R, Jurvelin J, Alhava E, Uusitupa M: Osteoporosis in adult patients with celiac disease. Bone. 1999, 24: 249-255. 10.1016/S8756-3282(98)00178-1.
Collin P, Maki M: Associated disorders in coeliac disease: clinical aspects. Scand J Gastroenterol. 1994, 29: 769-775.
Bottaro G, Cataldo F, Rotolo N, Spina M, Corazza GR: The clinical pattern of subclinical silent celiac disease: An analysis on 1026 consecutive cases. Am J Gastroenterol. 1999, 94: 691-696.
Fasano A: Celiac disease-how to handle a clinical chameleon. N Engl J Med. 2003, 348: 2568-2570. 10.1056/NEJMe030050.
Collin P, Kaukinen K, Maki M: Clinical features of celiac disease today. Dig Dis. 1999, 17: 100-106. 10.1159/000016911.
Weinstein WM: Latent celiac sprue. Gastroenterology. 1974, 66: 489-493.
Holmes GK: Potential and latent coeliac disease. Eur J Gastroenterol Hepatol. 2001, 13: 1057-1060. 10.1097/00042737-200109000-00010.
Maki M, Holm K, Koskimies S, Hallstrom O, Visakorpi JK: Normal small bowel biopsy followed by coeliac disease. Arch Dis Child. 1990, 65: 1137-1141.
Maki M, Holm K, Collin P, Savilahti E: Increase in g/d T cell receptor bearing lymphocytes in normal small bowel mucosa in latent coeliac disease. Gut. 1991, 32: 1412-1414.
Collin P, Helin H, Maki M, Hallstrom O, Karvonen AL: Follow-up of patients positive in reticulin and gliadin antibody tests with normal small-bowel biopsy findings. Scand J Gastroenterol. 1993, 28: 595-598.
Picarelli A, Maiuri L, Mazzilli MC, Coletta S, Ferrante P, Di Giovambattista F, Greco M, Torsoli A, Auricchio S: Gluten-sensitive disease with mild enteropathy. Gastroenterology. 1996, 111: 608-616. 10.1053/gast.1996.v111.pm8780564.
Ferguson A, Arranz E, O'Mahony S: Clinical and pathological spectrum of coeliac disease – active, silent, latent, potential. Gut. 1993, 34: 150-151.
Marsh MN: Clinical and pathological spectrum of coeliac disease. Gut. 1993, 34: 1740-
Wahnschaffe U, Ullrich R, Riecken EO, Schulzke JD: Celiac disease-like abnormalities in a subgroup of patients with irritable bowel syndrome. Gastroenterology. 2001, 121: 1329-1338. 10.1053/gast.2001.29572.
Zimmer KP: Klinische Bedeutung nichtklassischer Zöliakieformen. Deutsches Ärzteblatt. 2001, 98: A3285-A3292.
Holmes GK: Non-malignant complications of coeliac disease. Acta Paediatr Suppl. 1996, 412: 68-75.
Kumar V, Rajadhyaksha M, Wortsman J: Celiac disease-associated autoimmune endocrinopathies. Clin Diagn Lab Immunol. 2001, 8: 678-685. 10.1128/CDLI.8.4.678-685.2001.
Collin P, Kaukinen K, Valimaki M, Salmi J: Endocrinological disorders and celiac disease. Endocr Rev. 2002, 23: 464-483. 10.1210/er.2001-0035.
Alaedini A, Green PH: Narrative review: celiac disease: understanding a complex autoimmune disorder. Ann Intern Med. 2005, 142: 289-298.
Crabbe PA, Heremans JF: Selective IgA deficiency with steatorrhoea. A new syndrome. Am J Med. 1967, 42: 319-326. 10.1016/0002-9343(67)90031-9.
Collin P, Maki M, Keyrilainen O, Hallstrom O, Reunala T, Pasternack A: Selective IgA deficiency and coeliac disease. Scand J Gastroenterol. 1992, 27: 367-371.
Holmes GK, Prior P, Lane MR, Pope D, Allan RN: Malignancy in coeliac disease-effect of a gluten free diet. Gut. 1989, 30: 333-338.
Askling J, Linet M, Gridley G, Halstensen TS, Ekstrom K, Ekbom A: Cancer incidence in a population-based cohort of individuals hospitalized with Celiac disease or dermatitis herpetiformis. Gastroenterology. 2002, 123: 1428-1435. 10.1053/gast.2002.36585.
Loftus CG, Loftus EV Jr: Cancer risk in celiac disease. Gastroenterology. 2002, 123: 1726-1729. 10.1053/gast.2002.36850.
Marsh MN, Crowe PT: Morphology of the mucosal lesion in gluten sensitivity. Baillieres Clin Gastroenterol. 1995, 9: 273-293. 10.1016/0950-3528(95)90032-2.
Iltanen S, Holm K, Ashorn M, Ruuska T, Laippala P, Maki M: Changing jejunal g/d T cell receptor (TCR)-bearing intraepithelial lymphocyte density in coeliac disease. Clin Exp Immunol. 1999, 117: 51-55. 10.1046/j.1365-2249.1999.00948.x.
Holtmeier W, Rowell DL, Nyberg A, Kagnoff MF: Distinct d T cell receptor repertoires in monozygotic twins concordant for coeliac disease. Clin Exp Immunol. 1997, 107: 148-157. 10.1046/j.1365-2249.1997.d01-887.x.
Kaukinen K, Maki M, Partanen J, Sievanen H, Collin P: Celiac disease without villous atrophy: revision of criteria called for. Dig Dis Sci. 2001, 46: 879-887. 10.1023/A:1010729207320.
Marsh MN: Gluten, major histocompatibility complex, and the small intestine. A molecular and immunobiologic approach to the spectrum of gluten sensitivity ('celiac sprue'). Gastroenterology. 1992, 102: 330-354.
Oberhuber G, Granditsch G, Vogelsang H: The histopathology of coeliac disease: time for a standardized report scheme for pathologists. Eur J Gastroenterol Hepatol. 1999, 11: 1185-1194.
Maki M, Hallstrom O, Huupponen T, Vesikari T, Visakorpi JK: Increased prevalence of coeliac disease in diabetes. Arch Dis Child. 1984, 59: 739-742.
Holtmeier W, Caspary WF: Antibody diagnosis in sprue/celiac diseas. Z Gastroenterol. 1998, 36: 587-597.
Murray JA, Green PH: Biopsy is the gold standard of diagnosis of celiac sprue. Gastroenterology. 1999, 116: 1273-1274. 10.1016/S0016-5085(99)70040-5.
Rostami K, Kerckhaert J, Tiemessen R, von Blomberg BM, Meijer JW, Mulder CJ: Sensitivity of antiendomysium and antigliadin antibodies in untreated celiac disease: disappointing in clinical practice. Am J Gastroenterol. 1999, 94: 888-894. 10.1111/j.1572-0241.1999.983_f.x.
Dieterich W, Laag E, Schopper H, Volta U, Ferguson A, Gillett H, Riecken EO, Schuppan D: Autoantibodies to tissue transglutaminase as predictors of celiac disease. Gastroenterology. 1998, 115: 1317-1321. 10.1016/S0016-5085(98)70007-1.
Sblattero D, Berti I, Trevisiol C, Marzari R, Tommasini A, Bradbury A, Fasano A, Ventura A, Not T: Human recombinant tissue transglutaminase ELISA: an innovative diagnostic assay for celiac disease. Am J Gastroenterol. 2000, 95: 1253-1257. 10.1111/j.1572-0241.2000.02018.x.
Sulkanen S, Halttunen T, Laurila K, Kolho KL, Korponay-Szabo IR, Sarnesto A, Savilahti E, Collin P, Maki M: Tissue transglutaminase autoantibody enzyme-linked immunosorbent assay in detecting celiac disease. Gastroenterology. 1998, 115: 1322-1328. 10.1016/S0016-5085(98)70008-3.
Tesei N, Sugai E, Vazquez H, Smecuol E, Niveloni S, Mazure R, Moreno ML, Gomez JC, Maurino E, Bai JC: Antibodies to human recombinant tissue transglutaminase may detect coeliac disease patients undiagnosed by endomysial antibodies. Aliment Pharmacol Ther. 2003, 17: 1415-1423. 10.1046/j.1365-2036.2003.01595.x.
Leon F, Pena R, Camarero C, Sanchez L, Eiras P, Del Amo A, Bootello A, Roy G: Limitations of anti-guinea pig liver transglutaminase IgA in screening of celiac disease. Gastroenterology. 2001, 120: 586-587.
Schuppan D, Hahn EG: IgA anti-tissue transglutaminase: setting the stage for coeliac disease screening. Eur J Gastroenterol Hepatol. 2001, 13: 635-637. 10.1097/00042737-200106000-00004.
Osman AA, Richter T, Stern M, Conrad K, Henker J, Brandsch C, Zimmer KP, Mothes T: Production of recombinant human tissue transglutaminase using the baculovirus expression system, and its application for serological diagnosis of coeliac disease. Eur J Gastroenterol Hepatol. 2002, 14: 1217-1223. 10.1097/00042737-200211000-00009.
Wong RC, Wilson RJ, Steele RH, Radford-Smith G, Adelstein S: A comparison of 13 guinea pig and human anti-tissue transglutaminase antibody ELISA kits. J Clin Pathol. 2002, 55: 488-494.
Freeman HJ: Strongly positive tissue transglutaminase antibody assays without celiac disease. Can J Gastroenterol. 2004, 18: 25-28.
Hill ID, Dirks MH, Liptak GS, Colletti RB, Fasano A, Guandalini S, Hoffenberg EJ, Horvath K, Murray JA, Pivor M, Seidman EG: Guideline for the diagnosis and treatment of celiac disease in children: recommendations of the North American Society for Pediatric Gastroenterology, Hepatology and Nutrition. J Pediatr Gastroenterol Nutr. 2005, 40: 1-19. 10.1097/00005176-200501000-00001.
Schuppan D: Current concepts of celiac disease pathogenesis. Gastroenterology. 2000, 119: 234-242. 10.1053/gast.2000.8521.
Collin P, Kaukinen K, Vogelsang H, Korponay-Szabo I, Sommer R, Schreier E, Volta U, Granito A, Veronesi L, Mascart F, Ocmant A, Ivarsson A, Lagerqvist C, Burgin-Wolff A, Hadziselimovic F, Furlano RI, Sidler MA, Mulder CJ, Goerres MS, Mearin ML, Ninaber MK, Gudmand-Hoyer E, Fabiani E, Catassi C, Tidlund H, Alainentalo L, Maki M: Antiendomysial and antihuman recombinant tissue transglutaminase antibodies in the diagnosis of coeliac disease: a biopsy-proven European multicentre study. Eur J Gastroenterol Hepatol. 2005, 17: 85-91. 10.1097/00042737-200501000-00017.
Korponay-Szabo IR, Dahlbom I, Laurila K, Koskinen S, Woolley N, Partanen J, Kovacs JB, Maki M, Hansson T: Elevation of IgG antibodies against tissue transglutaminase as a diagnostic tool for coeliac disease in selective IgA deficiency. Gut. 2003, 52: 1567-1571. 10.1136/gut.52.11.1567.
Lock R, Stevens S, Pitcher MC, Unsworth DJ: Is immunoglobulin A anti-tissue transglutaminase antibody a reliable serological marker of coeliac disease?. Eur J Gastroenterol Hepatol. 2004, 16: 467-470. 10.1097/00042737-200405000-00005.
Fabiani E, Catassi C: The serum IgA class anti-tissue transglutaminase antibodies in the diagnosis and follow up of coeliac disease. Results of an international multi-centre study. International Working Group on Eu-tTG. Eur J Gastroenterol Hepatol. 2001, 13: 659-665. 10.1097/00042737-200106000-00008.
Cataldo F, Lio D, Marino V, Picarelli A, Ventura A, Corazza GR, the Working Groups on Celiac Disease of SIGEP and Club del Tenue: IgG(1) antiendomysium and IgG antitissue transglutaminase (anti-tTG) antibodies in coeliac patients with selective IgA deficiency. Gut. 2000, 47: 366-369. 10.1136/gut.47.3.366.
Sugai E, Selvaggio G, Vazquez H, Viola M, Mazure R, Pizarro B, Smecuol E, Flores D, Pedreira S, Maurino E, Gomez JC, Bai JC: Tissue transglutaminase antibodies in celiac disease: assessment of a commercial kit. Am J Gastroenterol. 2000, 95: 2318-2322. 10.1111/j.1572-0241.2000.02259.x.
Kaukinen K, Sulkanen S, Maki M, Collin P: IgA-class transglutaminase antibodies in evaluating the efficacy of gluten-free diet in coeliac disease. Eur J Gastroenterol Hepatol. 2002, 14: 311-315. 10.1097/00042737-200203000-00017.
Tursi A, Brandimarte G, Giorgetti GM: Lack of usefulness of anti-transglutaminase antibodies in assessing histologic recovery after gluten-free diet in celiac disease. J Clin Gastroenterol. 2003, 37: 387-391. 10.1097/00004836-200311000-00007.
Collin P, Maki M, Kaukinen K: Complete small intestine mucosal recovery is obtainable in the treatment of celiac disease. Gastrointest Endosc. 2004, 59: 158-159. 10.1016/S0016-5107(03)01311-7.
Vahedi K, Mascart F, Mary JY, Laberenne JE, Bouhnik Y, Morin MC, Ocmant A, Velly C, Colombel JF, Matuchansky C: Reliability of antitransglutaminase antibodies as predictors of gluten-free diet compliance in adult celiac disease. Am J Gastroenterol. 2003, 98: 1079-1087. 10.1111/j.1572-0241.2003.07284.x.
National Institutes of Health Consensus Development Conference Statement on Celiac Disease, June 28–30, 2004. Gastroenterology. 128: S1-S9. 10.1053/j.gastro.2005.02.007.
Kasarda DD, Okita TW, Bernardin JE, Baecker PA, Nimmo CC, Lew EJ, Dietler MD, Greene FC: Nucleic acid (cDNA) and amino acid sequences of a-type gliadins from wheat (Triticum aestivum L.). Proc Natl Acad Sci USA. 1984, 81: 4712-
Hardman CM, Garioch JJ, Leonard JN, Thomas HJ, Walker MM, Lortan JE, Lister A, Fry L: Absence of toxicity of oats in patients with dermatitis herpetiformis. N Engl J Med. 1997, 337: 1884-1887. 10.1056/NEJM199712253372604.
Janatuinen EK, Pikkarainen PH, Kemppainen TA, Kosma VM, Jarvinen RM, Uusitupa MI, Julkunen RJ: A comparison of diets with and without oats in adults with celiac disease. N Engl J Med. 1995, 333: 1033-1037. 10.1056/NEJM199510193331602.
Janatuinen EK, Kemppainen TA, Pikkarainen PH, Holm KH, Kosma VM, Uusitupa MI, Maki M, Julkunen RJ: Lack of cellular and humoral immunological responses to oats in adults with coeliac disease. Gut. 2000, 46: 327-331. 10.1136/gut.46.3.327.
Janatuinen EK, Kemppainen TA, Julkunen RJK, Kosma VM, Maki M, Heikkinen M, Uusitupa MIJ: No harm from five year ingestion of oats in coeliac disease. Gut. 2002, 50: 332-335. 10.1136/gut.50.3.332.
Hogberg L, Laurin P, Falth-Magnusson K, Grant C, Grodzinsky E, Jansson G, Ascher H, Browaldh L, Hammersjo JA, Lindberg E, Myrdal U, Stenhammar L: Oats to children with newly diagnosed coeliac disease: a randomised double blind study. Gut. 2004, 53: 649-654. 10.1136/gut.2003.026948.
Vader LW, de Ru A, van der WY, Kooy YM, Benckhuijsen W, Mearin ML, Drijfhout JW, van Veelen P, Koning F: Specificity of tissue transglutaminase explains cereal toxicity in celiac disease. J Exp Med. 2002, 195: 643-649. 10.1084/jem.20012028.
Lundin KE, Nilsen EM, Scott HG, Loberg EM, Gjoen A, Bratlie J, Skar V, Mendez E, Lovik A, Kett K: Oats induced villous atrophy in coeliac disease. Gut. 2003, 52: 1649-1652. 10.1136/gut.52.11.1649.
Peraaho M, Kaukinen K, Mustalahti K, Vuolteenaho N, Maki M, Laippala P, Collin P: Effect of an oats-containing gluten-free diet on symptoms and quality of life in coeliac disease. A randomized study. Scand J Gastroenterol. 2004, 39: 27-31.
Walker-Smith JA, Guandalini S, Schmitz J, Shmerling DH, Visakorpi JK: Revised criteria for diagnosis of coeliac disease. Arch Dis Child. 1990, 65: 909-911.
Selby WS, Painter D, Collins A, Faulkner-Hogg KB, Loblay RH: Persistent mucosal abnormalities in coeliac disease are not related to the ingestion of trace amounts of gluten. Scand J Gastroenterol. 1999, 34: 909-914. 10.1080/003655299750025390.
Lee SK, Lo W, Memeo L, Rotterdam H, Green PHR: Duodenal histology in patients with celiac disease after treatment with a gluten-free diet. Gastrointes Endoscopy. 2003, 57: 187-191.
Mustalahti K, Collin P, Sievanen H, Salmi J, Maki M: Osteopenia in patients with clinically silent coeliac disease warrants screening. Lancet. 1999, 354: 744-745. 10.1016/S0140-6736(99)01990-X.
Ventura A, Magazzu G, Greco L: Duration of exposure to gluten and risk for autoimmune disorders in patients with celiac disease. SIGEP Study Group for Autoimmune Disorders in Celiac Disease. Gastroenterology. 1999, 117: 297-303. 10.1053/gast.1999.0029900297.
Sategna GC, Solerio E, Scaglione N, Aimo G, Mengozzi G: Duration of gluten exposure in adult coeliac disease does not correlate with the risk for autoimmune disorders. Gut. 2001, 49: 502-505. 10.1136/gut.49.4.502.
Viljamaa M, Kaukinen K, Huhtala H, Kyronpalo S, Rasmussen M, Collin P: Coeliac disease, autoimmune diseases and gluten exposure. Scand J Gastroenterol. 2005, 40: 437-443.
Abdulkarim AS, Burgart LJ, See J, Murray JA: Etiology of nonresponsive celiac disease: Results of a systematic approach. Am J Gastroenterol. 2002, 97: 2016-2021. 10.1111/j.1572-0241.2002.05917.x.
Olesen M, Eriksson S, Bohr J, Jarnerot G, Tysk C: Lymphocytic colitis: a retrospective clinical study of 199 Swedish patients. Gut. 2004, 53: 536-541. 10.1136/gut.2003.023440.
Tursi A, Brandimarte G, Giorgetti GM: High prevalence of small intestinal bacterial overgrowth in celiac patients with persistence of gastrointestinal symptoms after gluten withdrawal. Am J Gastroenterol. 2003, 98: 839-843. 10.1111/j.1572-0241.2003.07379.x.
Freeman HJ, Chiu BK: Small bowel malignant lymphoma complicating celiac sprue and the mesenteric lymph node cavitation syndrome. Gastroenterology. 1986, 90: 2008-2012.
Ryan BM, Kelleher D: Refractory celiac disease. Gastroenterology. 2000, 119: 243-251. 10.1053/gast.2000.8530.
Fasano A: European and North American populations should be screened for coeliac disease. Gut. 2003, 52: 168-169. 10.1136/gut.52.2.168.
Kumar PJ: European and North American populations should be screened for coeliac disease. Gut. 2003, 52: 170-171. 10.1136/gut.52.2.170.
Card TR, West J, Holmes GK: Risk of malignancy in diagnosed coeliac disease: a 24-year prospective, population-based, cohort study. Aliment Pharmacol Ther. 2004, 20: 769-775. 10.1111/j.1365-2036.2004.02177.x.
Catassi C, Fabiani E, Corrao G, Barbato M, De Renzo A, Carella AM, Gabrielli A, Leoni P, Carroccio A, Baldassarre M, Bertolani P, Caramaschi P, Sozzi M, Guariso G, Volta U, Corazza GR: Risk of non-Hodgkin lymphoma in celiac disease. JAMA. 2002, 287: 1413-1419. 10.1001/jama.287.11.1413.
Molberg O, Solheim FN, Jensen T, Lundin KE, Arentz-Hansen H, Anderson OD, Kjersti UA, Sollid LM: Intestinal T-cell responses to high-molecular-weight glutenins in celiac disease. Gastroenterology. 2003, 125: 337-344. 10.1016/S0016-5085(03)00890-4.
Molberg O, Uhlen AK, Jensen T, Flaete NS, Fleckenstein B, Arentz-Hansen H, Raki M, Lundin KE, Sollid LM: Mapping of gluten T-cell epitopes in the bread wheat ancestors: Implications for celiac disease. Gastroenterology. 2005, 128: 393-401. 10.1053/j.gastro.2004.11.003.
Sollid LM: Molecular basis of celiac disease. Annu Rev Immunol. 2000, 18: 53-81. 10.1146/annurev.immunol.18.1.53.
Lundin KE, Scott H, Hansen T, Paulsen G, Halstensen TS, Fausa O, Thorsby E, Sollid LM: Gliadin-specific, HLA-DQ(a 1*b 1*0201) restricted T cells isolated from the small intestinal mucosa of celiac disease patients. J Exp Med. 0501, 178: 187-196. 10.1084/jem.178.1.187.
Molberg O, Mcadam SN, Korner R, Quarsten H, Kristiansen C, Madsen L, Fugger L, Scott H, Noren O, Roepstorff P, Lundin KE, Sjostrom H, Sollid LM: Tissue transglutaminase selectively modifies gliadin peptides that are recognized by gut-derived T cells in celiac disease. Nat Med. 1998, 4: 713-717. 10.1038/nm0698-713.
van de Wal Y, Kooy Y, van Veelen P, Pena S, Mearin L, Papadopoulos G, Koning F: Selective deamidation by tissue transglutaminase strongly enhances gliadin-specific T cell reactivity. J Immunol. 1998, 161: 1585-1588.
Quarsten H, Molberg O, Fugger L, Mcadam SN, Sollid LM: HLA binding and T cell recognition of a tissue transglutaminase-modified gliadin epitope. Eur J Immunol. 1999, 29: 2506-2514. 10.1002/(SICI)1521-4141(199908)29:08<2506::AID-IMMU2506>3.0.CO;2-9.
Molberg O, McAdam S, Lundin KEA, Kristiansen C, Arentz-Hansen H, Kett K, Sollid LM: T cells from celiac disease lesions recognize gliadin epitopes deamidated in situ by endogenous tissue transglutaminase. Eur J Immunol. 2001, 31: 1317-1323. 10.1002/1521-4141(200105)31:5<1317::AID-IMMU1317>3.0.CO;2-I.
Benahmed M, Mention JJ, Matysiak-Budnik T, Cerf-Bensussan N: Celiac disease: A future without gluten-free diet?. Gastroenterology. 2003, 125: 1264-1267. 10.1016/j.gastro.2003.07.002.
Dieterich W, Ehnis T, Bauer M, Donner P, Volta U, Riecken EO, Schuppan D: Identification of tissue transglutaminase as the autoantigen of celiac disease. Nat Med. 1997, 3: 797-801. 10.1038/nm0797-797.
Sollid LM, Molberg O, McAdam S, Lundin KE: Autoantibodies in coeliac disease: tissue transglutaminase-guilt by association?. Gut. 1997, 41: 851-852.
Halttunen T, Maki M: Serum immunoglobulin A from patients with celiac disease inhibits human T84 intestinal crypt epithelial cell differentiation. Gastroenterology. 1999, 116: 566-572. 10.1016/S0016-5085(99)70178-2.
Arentz-Hansen H, Mcadam SN, Molberg O, Fleckenstein B, Lundin KE, Jorgensen TJ, Jung G, Roepstorff P, Sollid LM: Celiac lesion T cells recognize epitopes that cluster in regions of gliadins rich in proline residues. Gastroenterology. 2002, 123: 803-809. 10.1053/gast.2002.35381.
Vader W, Kooy Y, van Veelen P, de Ru A, Harris D, Benckhuijsen W, Pena S, Mearin L, Drijfhout JW, Koning F: The gluten response in children with celiac disease is directed toward multiple gliadin and glutenin peptides. Gastroenterology. 2002, 122: 1729-1737. 10.1053/gast.2002.33606.
Holtmeier W, Caspary WF: Identification of toxic gliadin fragments – new therapeutic options for patients suffering from coeliac disease (nontropical sprue)?. Z Gastroenterol. 2002, 40: 999-1000. 10.1055/s-2002-36155.
Schuppan D, Hahn EG: Biomedicine: Gluten and the gut-lessons for immune regulation. Science. 2002, 297: 2218-2220. 10.1126/science.1077572.
Shan L, Molberg O, Parrot I, Hausch F, Filiz F, Gray GM, Sollid LM, Khosla C: Structural basis for gluten intolerance in celiac sprue. Science. 2002, 297: 2275-2279. 10.1126/science.1074129.
Anderson RP, Degano P, Godkin AJ, Jewell DP, Hill AV: In vivo antigen challenge in celiac disease identifies a single transglutaminase-modified peptide as the dominant A-gliadin T-cell epitope. Nat Med. 2000, 6: 337-342. 10.1038/73200.
Arentz-Hansen H, Korner R, Molberg O, Quarsten H, Vader W, Kooy YM, Lundin KE, Koning F, Roepstorff P, Sollid LM, Mcadam SN: The intestinal T cell response to a-gliadin in adult celiac disease is focused on a single deamidated glutamine targeted by tissue transglutaminase. J Exp Med. 2000, 191: 603-612. 10.1084/jem.191.4.603.
Mcadam SN, Sollid LM: Getting to grips with gluten. Gut. 2000, 47: 743-745. 10.1136/gut.47.6.743.
Hausch F, Shan L, Santiago NA, Gray GM, Khosla C: Intestinal digestive resistance of immunodominant gliadin peptides. Am J Physiol Gastrointest Liver Physiol. 2002, 283: G996-G1003.
Celiac Sprue Research Foundation. [http://www.celiacsprue.org/]
Koning F, Vader W: Gluten peptides and celiac disease. Science. 2003, 299: 513-515. 10.1126/science.299.5606.513.
Wong RC, Steele RH, Reeves GE, Wilson RJ, Pink A, Adelstein S: Antibody and genetic testing in coeliac disease. Pathology. 2003, 35: 285-304. 10.1080/00313020307527.
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Holtmeier, W., Caspary, W.F. Celiac disease. Orphanet J Rare Dis 1, 3 (2006). https://doi.org/10.1186/1750-1172-1-3
- Celiac Disease
- Villous Atrophy
- Celiac Disease Patient
- Tissue Transglutaminase
- Tropical Sprue