- Open Access
Botulinum toxin injections as an effective treatment for patients with intertriginous Hailey-Hailey or Darier disease: an open-label 6-month pilot interventional study
Orphanet Journal of Rare Diseases volume 16, Article number: 93 (2021)
Patients with Hailey-Hailey and Darier diseases present with disabling inflammatory lesions located in large skin folds, which are often exacerbated or induced by sweating. Quality of life is highly impaired because of pain and recurrent skin infections. An improvement in skin lesions after botulinum toxin A injections has previously been reported in some patients but no prospective interventional studies are available. The aim of this open-label, 6-month, interventional pilot study (NCT02782702) was to evaluate the effectiveness and safety of botulinum toxin A for patients with moderate to very severe skin lesions located in folds.
Thirty patients (26 Hailey-Hailey/4 Darier) were included. Botulinum toxin A proved effective within the first month in two-thirds of patients, taking all study parameters (itchiness, cutaneous pain, sweating and odour, infections, psychosocial impairment and quality of life) into account and persisted during the 6-month follow-up period. No patient was classed as a BtxA non-responder, but 11 (37%) Hailey-Hailey patients (the most severe ones), experienced a relapse during the study. No serious side effects were reported. Mild transient clear fluid discharge at the site of the injections was reported for 27% of patients.
Botulinic toxin seems to be an effective and safe treatment for Hailey-Hailey and Darier diseases. Nevertheless, it may prove insufficient for the severest of Hailey-Hailey cases and could be considered as supplementary to other conventional treatments. Further studies are required to confirm our results on larger Darier cohorts.
Darier disease (DD) and Hailey-Hailey disease (HHD) are two rare genetic diseases sharing some clinical (recurrent inflammatory erythematous plaques with a predilection for the skin folds), histopathological (acantholytic dyskeratosis) and genetic (inherited as autosomal-dominant traits, mutations in genes encoding for Ca2+ ATPases, ATP2A2 for DD and ATP2C1 for HHD) similarities . DD is mainly defined by warty papules in seborrheic and flexural areas, whereas the cardinal symptoms of HHD are vesicles and erosions in flexural skin . Lesions are intermittent or permanent but acute exacerbations occur, especially during hot seasons, with a possible worsening with sweating. For both diseases, quality of life (QOL) is impaired because of troublesome symptoms such as pain, itchiness and a predilection for cutaneous infections [3,4,5].
There are currently no curative treatments. Several therapeutic options, like topical agents [6,7,8,9,10], systemic therapies [11,12,13,14,15,16,17,18] and interventional treatments [19,20,21,22,23,24,25,26,27,28,29], are available but HHD and DD are often difficult to control. Botulinum toxin type A (BtxA) may be another option. BtxA has proved effective outside the field of Dermatology. In Dermatology, intracutaneous injections of BtxA have been used to treat focal hyperhidrosis [30,31,32] and skin conditions such as pachyonychia congenita . BtxA blocks the release of acetylcholine from the sympathetic nerve fibres that stimulates the eccrine sweat glands and causes a localised reduction in sweating. An improvement in skin lesions following BtxA injections has previously been reported in a few patients suffering from both DD and HHD [34,35,36,37,38,39,40,41,42,43], but no prospective interventional studies are available. The aim of this study was to evaluate the effect of BtxA injections in the flexural skin of HHD and DD patients.
Material and methods
This was an open-label, 6-month, interventional pilot study conducted in an expert centre. The study protocol was approved by the Institutional Ethics Committee (Comité de Protection des Personnes—Aquitaine, Bordeaux, France) and the French National Agency for Medicines and Health Products Safety, ANSM (Agence Nationale de Sécurité du Médicament et des produits de santé). The study was conducted in accordance with the principles of the 1975 Declaration of Helsinki, revised in 1983, and registered under the ClinicalTrials.gov identifier NCT02782702.
According to pilot study methodology , 30 patients were expected to be enrolled in this study. Eligible patients were aged ≥ 18 years and had a clinically and biopsy-proven diagnosis of DD or HHD. At baseline, patients were required to present at least one lesion located in the large fold areas (inguinal, axillary, mammary and abdominal), ranging from moderately severe to very severe (see section on outcomes). Exclusion criteria were provided as Additional file 1. Written consent was obtained from all patients.
Each patient was scheduled to attend four visits. At the first visit, each patient received intradermal BtxA injections (BotoxR) on selected areas presenting moderate to very severe lesions (see section on outcomes). These injections were performed under local anaesthesia (lidocaine prilocaine 5% cream, applied one hour earlier). Not more than one BtxA (200-unit) vial was used per patient. The BtxA vial was diluted with 8 mL of sterile, preservative-free physiological saline solution (final concentration: 2.5 units/0.1 ml). The areas to be injected were divided into a grid of 1 to 2 cm. Using a 30-gauge needle, 2 mL (50 units) were then administered intradermally, divided into a constant volume of 0.1 mL injected into the centre of each grid, resulting in approximately 20 intradermal injections over the entire treated area. The target concentration was 50 IU per 100 cm2. There were 2 follow-up visits (1 and 3 months (M): M1 and M3), and one end-of-study visit (M6). Patients were instructed not to use any concomitant medication throughout the treatment period, except for emollients and antiseptics. In the event of relapse, defined as the occurrence of new skin lesions in the treated areas necessitating one of these prohibited treatments, the patient was excluded from the study (but still followed-up for tolerance outcomes).
Several parameters were evaluated at each visit: The first parameter was QOL, evaluated by the Dermatology Life Quality Index (DLQI)  (a score > 10 is associated with a severe or very severe impact on QOL ). We also evaluated psychosocial impairment using HidroQoL ; debilitating symptoms (itchiness, cutaneous pain, sweating and odour) using Visual Analogue Scales (VAS) (ranging from 0 (none) to 10 (worst case scenario)); and the number of infectious skin episodes (in the six months before and after the injections). The total surface area (cm2) was evaluated by totalling the surface of all selected areas. The severity of the lesions was evaluated on an individual basis using a 5-point photographic scale (no lesion, mild, moderate, severe, very severe), generated by the investigators and five other independent experts (see Additional file 2). Global severity was defined as the average of the severity of each selected area. Clinical improvement was assessed by the treating investigator (AM) as well as a blinded investigator (ID) at M1, M3 and M6, using the IGA score (Improvement Global Assessment) , a validated 5-point scale, ranging from 0 to 4 (no improvement or exacerbation of the treated lesions, slight improvement, moderate improvement, significant improvement, complete disappearance of all lesions). In the event of discordance, moderation analysis were carried out. Patients were considered in treatment failure if the treated area did not improve throughout the study (non-responders to BtxA) or if they relapsed (occurrence of new skin lesions in the treated areas warranting the use of a prohibited treatment). Finally, tolerance was assessed during the injections using a VAS quoting pain from 0 to 10 (0: no pain, 10: maximal pain), and throughout the study period by reporting side effects. Patient satisfaction was recorded at the end-of-study visit according to a 4-point scale (very satisfied, satisfied, somewhat satisfied, dissatisfied).
A descriptive analysis was performed at each evaluation time (baseline, M1, M3 and M6) for each assessment criterion (mean, standard deviation, minimum–maximum for continuous variables; proportion for categorical variables). Before/after comparisons (each evaluation time vs. baseline, no comparison between the different follow-up evaluation times) were then performed using a Wilcoxon test for paired values. Disease severity at baseline (defined by the total surface area and the global severity) was compared between relapsing and non-relapsing patients using a Mann–Whitney test. For all tests, a p value of < 0.05 was considered statistically significant. All statistical tests were performed with STATA software.
A total of 30 patients were enrolled in the study. Their characteristics are reported in Table 1. Most of the patients were suffering from HHD (n = 26, 87%) with an equal percentage of men and women, over 55 years old on average. Mean disease duration exceeded 25 years and all patients had received previous therapies.
The mean (± SD) number of selected areas per patient at baseline [min–max] was 3 (± 1.2) [1–7], with the axillary fold being the main focus (77% of patients). The mean (± SD) global severity score [min—max] was 2.9 (± 0.81) [2–4], corresponding to 17% moderate, 43% severe and 40% very severe lesions. The mean (± SD) total treated surface area per patient [min—max] was 272 cm2 (± 208.2) [43—995], resulting in a mean (± SD) dose of BtxA [min—max] of 125 IU (± 61.6) [20–200], with respect to the target concentration.
QOL was impaired for all patients, with a mean (± SD) DLQI score [min–max] of 13 (± 6.84) [1–26], and 20 (66.7%) patients reporting a severe to very severe impact on their QOL. Severe psychosocial impairment was also noted with a mean (± SD) HidroQoL score [min–max] of 18.3 (± 7.8) [5–35]. All (100%) patients reported debilitating functional symptoms, predominantly sweating (mean VAS (± SD) score [min–max] of 5.8 (± 2.65) [0–10]).
The effects of BtxA are presented in Tables 2a and b. A total of 20 (66.7%), 19 (63.2%) and 15 (50%) patients improved (IGA > 0), with a complete disappearance of the lesions in 6 (20%), 7 (23.3%) and 7 (23.3%) patients at M1, M3 and M6, respectively (Fig. 1). No patient was classed as a BtxA non-responder, but 11 (36.7%) patients (11 HHD) experienced a relapse during the study: 9 (30%), 0 (0%) and 2 (6.7%) patients at M1, M3 and M6, respectively. The condition of these HHD relapsing patients was more severe at baseline compared to non-relapsing patients, with a mean (± SD) global severity score [min–max] of 3.21 (± 0.65) [2.5–4] vs. 2.7 (± 0.64) [2–4], p = 0.05. Relapses were recorded in all locations and occurred in areas with very severe (45.5%), severe (45.5%) or moderate (9%) baseline lesions. The total surface area at baseline did not differ statistically between relapsing and non-relapsing patients (mean (± SD) total surface area [min–max] of 323 cm2 (± 294.8) [44–995] vs. 242.1 cm2 (± 137.9) [43–553], p = 0.81).
When considering the HHD and DD non-relapsing patients (Table 2a, Fig. 2), all study parameters were significantly improved by BtxA, with a reduction in values from 24 to 93%, compared to baseline. This reduction was observed from M1 onwards, and persisted over time, remaining significantly lower at M6 compared to baseline, for all values. Only sweating and odours tended to rise once again after M1. The results were similar when detailing per disease (Table 2b), even though the DD cohort is too small to perform statistical analysis separately. When focusing on HHD patients, the mean (± SD) total surface area per patient was reduced by 85% (p = 0.0003). In the same way, QOL improved substantially, with the DLQI score that reduced by more than two-thirds (p = 0.0003) at M6. Lastly, skin infections were virtually non-existent during the study period (0.33 (± 1.29), p = 0.01).
BtxA injections were fairly well tolerated by all patients, with a mean (± SD) pain score during the injections [min–max] of 3.58/10 (± 2.3) [0–8]. Seven patients (23%) experienced transient and very slight side effects during the injections (bleeding (16.5%), tingling sensations (6.5%)). Eight patients (27%) reported a mild, debilitating, clear fluid discharge from the treated areas starting within the first few days of injections and persisting for less than one month. No systemic side effects attributable to the BtxA injections were reported during patient follow-up. Finally, 12 patients (40%) declared that they were very satisfied with BtxA injections (9 (30%) satisfied/2 (6.7%) somewhat satisfied/7 (23.3%) dissatisfied, including 5 relapsing patients, whose dissatisfaction was related to discontinuation of their usual treatments, underlying insufficient global efficacy and relapse).
This is the first prospective study aiming to demonstrate the effectiveness and safety of BtxA injections in the management of DD and HHD. Except for the most severe HHD patients, BtxA is effective within the first month taking all study parameters into account including QOL, and continues to be effective during the 6-month follow-up period.
There are some limitations to our study: the small number of patients (especially with DD), thereby precluding some statistical analysis because of a lack of statistical power the pilot design preventing a control group (placebo group or intra-individual comparison between treated and untreated/placebo treated sides), the highly variable spontaneous course (especially of HHD) with exacerbations and remissions and the absence of information about late relapses (follow-up limited to 6 months). Given the lack of validated scores, we generated a 5-point photographic severity scale. No psychometric validation of this scale was performed in this study. Nevertheless, the fact that clinical severity (rated with this scale) and the other study parameters are moving in the same direction is indicative of the suitability of this tool.
Our results are difficult to compare with those of published small-scale retrospective case series or some clinical cases. In DD, BtxA was used in only three patients in whom a reduction in skin lesions in the treated areas was reported without any objective quantification [49,50,51]. In HHD, BtxA proved effective in 27 patients (small-scale retrospective case series including no more than eight patients  or clinical cases [34,35,36,37,38,39,40,41,42]). Full or partial remissions were reported using variable dosages of BtxA. Maintenance sessions with variable intervals were initiated for some patients because of relapses occurring after 4–17 months.
Our study confirms the constant, rapid and persistent effectiveness of BtxA with specific information on the improved parameters over a 6-month period. Our study is also the first to demonstrate an impact on QOL, deemed to be the most important parameter when assessing the impact of a new intervention .
An important aspect of our study is the characterisation of relapsing HHD patients, who mostly corresponded to the most severe cases. These relapses were mostly early-onset and may have been influenced by the discontinuation of previous therapies not permitted during the study (i.e. local steroids). For those patients, the dosage of BtxA may also have been too low. To our knowledge, no data are available regarding the management of HHD and DD with such higher dosages, but BtxA safety data in other diseases such as spasticity suggest that a maximum dose of 1000 IU per injection or 30 IU/kg per patient per injection (whichever is lowest) may be used . BtxA may also be used as a combined treatment. Surgical ablation which can cover much larger affected areas remains the treatment of choice in intertriginous HHD and DD diseases.
Similar to the literature, few slight side effects such as tingling and bleeding during the injections were reported in our study. Transient fluid discharge has not been previously reported. After questioning patients, this fluid discharge could be indicative of increased oozing from abraded skin.
This study extends the therapeutic use of BtxA beyond aesthetic medicine  and enhances our knowledge of the mechanism of BtxA. The fact that predilection for recurrent sweating and odours after M1 did not correlate with diminished efficacy may suggest that BtxA not only reduced sweating, but may have other biological effects in non-neuronal cells such as an anti-inflammatory effect .
In conclusion, BtxA seems to be a safe and effective treatment in the management of intertriginous HHD and DD, thereby expanding the treatment portfolio available. Nevertheless, it may prove inadequate for the severest of HHD cases and has to be used at higher dosages or in combination with other conventional treatments. Further studies are required to confirm our results on larger DD cohorts.
Availability of data and materials
The datasets generated, used and analysed during the current study are available from the corresponding author on reasonable request.
Agence Nationale de Sécurité du Médicament et des produits de santé
Botulinum toxin type A
Dermatology Life Quality Index
Improvement Global Assessment
Quality of life
Visual Analogue Scales
Nellen RG, Steijlen PM, van Steensel MA, et al. Mendelian disorders of cornification caused by defects in intracellular calcium pumps: mutation update and database for variants in ATP2A2 and ATP2C1 Associated with Darier Disease and Hailey-Hailey Disease. Hum Mutat. 2017;38:343–56.
Takagi A, Kamijo M, Ikeda S. Darier disease. J Dermatol. 2016;43:275–9.
Dodiuk-Gad R, Cohen-Barak E, Ziv M, et al. Health-related quality of life among Darier’s disease patients. J Eur Acad Dermatol Venereol. 2013;27:51–6.
Gisondi P, Sampogna F, Annessi G, et al. Severe impairment of quality of life in Hailey-Hailey disease. Acta Derm Venereol. 2005;85:132–5.
Harris A, Burge SM, Dykes PJ, Finlay AY. Handicap in Darier’s disease and Hailey-Hailey disease. Br J Dermatol. 1996;135:959–63.
Abe M, Yasuda M, Yokoyama Y, Ishikawa O. Successful treatment of combination therapy with tacalcitol lotion associated with sunscreen for localized Darier’s disease. J Dermatol. 2010;37:718–21.
Abe M, Inoue C, Yokoyama Y, Ishikawa O. Successful treatment of Darier’s disease with adapalene gel. Pediatr Dermatol. 2011;28:197–8.
Schmidt H, Ochsendorf FR, Wolter M, Geisslinger G, Ludwig RJ, Kaufmann R. Topical 5-fluorouracil in Darier disease. Br J Dermatol. 2008;158:1393–6.
Rubegni P, Poggiali S, Sbano P, et al. A case of Darier’s disease successfully treated with topical tacrolimus. J Eur Acad Dermatol Venereol. 2006;20:84–7.
Tchernev G, Cardoso JC. Familial benign chronic pemphigus (Hailey-Hailey Disease): use of topical immunomodulators as a modern treatment option. Rev Med Chil. 2011;139:633–7.
Le Saché-de PL, Raynaud E, Bouchardeau A, et al. Familial benign chronic pemphigus and doxycycline: a review of 6 cases. J Eur Acad Dermatol Venereol. 2014;28:370–3.
Letulé V, Herzinger T, Ruzicka T, Molin S. Treatment of Darier disease with oral alitretinoin. Clin Exp Dermatol. 2013;38:523–5.
Sárdy M, Ruzicka T. Successful therapy of refractory Hailey-Hailey disease with oral alitretinoin. Br J Dermatol. 2014;170:209–11.
Varada S, Ramirez-Fort MK, Argobi Y, Simkin AD. Remission of refractory benign familial chronic pemphigus (hailey-hailey disease) with the addition of systemic cyclosporine. J Cutan Med Surg. 2015;19:163–6.
Stewart LC, Yell J. Vulval Darier’s disease treated successfully with ciclosporin. J Obstet Gynaecol. 2008;28:108–9.
Ibrahim O, Hogan SR, Vij A, Fernandez AP. Low-dose naltrexone treatment of familial benign pemphigus (Hailey-Hailey Disease). JAMA Dermatol. 2017;153:1015–7.
Campbell V, McGrath C, Corry A. Low-dose naltrexone: a novel treatment for Hailey-Hailey disease. Br J Dermato. 2018;178:1196–8.
Albers LN, Arbiser JL, Feldman RJ. Treatment of Hailey-Hailey disease with low-dose naltrexone. JAMA Dermatol. 2017;153:1018–20.
Farahnik B, Blattner CM, Mortazie MB, et al. Interventional treatments for Hailey-Hailey disease. J Am Acad Dermatol. 2017;76:551–8.
Hamm H, Metze D, Brocker EB. Hailey disease. Eradication by dermabrasion. Arch Dermatol. 1994;130:1143–9.
Shons AR. Wide excision of perineal hailey-hailey disease with healing by secondary intention. Br J Plast Surg. 1989;42:230–2.
Hochwalt PC, Christensen KN, Cantwell SR, et al. Carbon dioxide laser treatment for Hailey-Hailey disease: a retrospective chart review with patient-reported outcomes. Int J Dermatol. 2015;54:1309–14.
Brown VL, Kelly SE, Burge SM, Walker NP. Extensive recalcitrant Darier disease successfully treated with laser ablation. Br J Dermatol. 2010;162:227–9.
Touma DJ, Krauss M, Feingold DS, Kaminer MS. Benign familial pemphigus (Hailey disease). Treatment with the pulsed carbon dioxide laser. Dermatol Surg. 1998;24:1411–4.
Alsahli M, Debu A, Girard C, et al. Is photodynamic therapy a relevant therapeutic option in refractory benign familial pemphigus (Hailey-Hailey disease)? A series of eight patients. J Dermatolog Treat. 2017;28:678–82.
Exadaktylou D, Kurwa HA, Calonje E, Barlow RJ. Treatment of Darier’s disease with photodynamic therapy. Br J Dermatol. 2003;149:606–10.
Fernández Guarino M, Ryan AM, Harto A, et al. Experience with photodynamic therapy in Hailey-Hailey disease. J Dermatolog Treat. 2008;19:288–90.
Kittridge A, Wahlgren C, Fuhrer R, et al. Treatment of recalcitrant Darier’s disease with electron beam therapy. Dermatol Ther. 2010;23:302–4.
Narbutt J, Chrusciel A, Rychter A, et al. Persistent improvement of previously recalcitrant Hailey-Hailey disease with electron beam radiotherapy. Acta Derm Venereol. 2010;90:179–82.
Heckmann M, Ceballos-Baumann AO, Plewig G. Hyperhidrosis Study Group Botulinum toxin A for axillary hyperhidrosis (excessive sweating). N Engl J Med. 2001;344:488–93.
Vergilis-Kalner IJ. Same-patient prospective comparison of botox versus dysport for the treatment of primary axillary hyperhidrosis and review of literature. J Drugs Dermatol. 2011;10:1013–5.
Anders D, Moosbauer S, Naumann MK, Hamm H. Craniofacial hyperhidrosis successfully treated with botulinum toxin type A. Eur J Dermatol. 2008;18:87–8.
Grando SA, Zachary CB. The non-neuronal and nonmuscular effects of botulinum toxin: an opportunity for a deadly molecule to treat disease in the skin and beyond. Br J Dermatol. 2018;178:1011–9.
Bedi M, Taylor AL. Recalcitrant Hailey-Hailey disease responds to oral tacrolimus and botulinum toxin type A. Cutis. 2015;96:E14–6.
Bessa GR, Grazziotin TC, Manzoni AP, et al. Hailey-Hailey disease treatment with botulinum toxin type A. An Bras Dermatol. 2010;85:717–22.
Ho D, Jagdeo J. Successful botulinum toxin (onabotulinumtoxinA) treatment of Hailey-Hailey disease. J Drugs Dermatol. 2015;14:68–70.
Kang NG, Yoon TJ, Kim TH. Botulinum toxin type A as an effective adjuvant therapy for Hailey-Hailey disease. Dermatol Surg. 2002;28:543.
Koeyers WJ, Van Der Geer S, Krekels G. Botulinum toxin type A as an adjuvant treatment modality for extensive Hailey-Hailey disease. J Dermatolog Treat. 2008;19:251–4.
Konrad H, Karamfilov T, Wollina U. Intracutaneous botulinum toxin A versus ablative therapy of Hailey-Hailey disease — a case report. J Cosmet Laser Ther. 2001;3:181–4.
Lapiere JC, Hirsh A, Gordon KB, et al. Botulinum toxin type A for the treatment of axillary Hailey-Hailey disease. Dermatol Surg. 2000;26:371–4.
López-Ferrer A, Alomar A. Botulinum toxin A for the treatment of familial benign pemphigus. Actas Dermosifiliogr. 2012;103:532–5.
Charlton OA, Stewart TJ, Rosen RH. Treatment of Hailey-Hailey disease with botulinum toxin. Austral J Dermatol. 2018;59:229–31.
Dousset L, Pham-Ledard A, Doutre MS, et al. Treatment of Hailey-Hailey disease with botulinum toxin: a retrospective study of 8 cases]. Ann Dermatol Venereol. 2017;144:599–606.
Lancaster GA, Dodd S, Williamson PR. Design and analysis of pilot studies: recommendations for good practice. J Eval Clin Pract. 2004;10:307–12.
Finlay AY, Khan GK. Dermatology Life Quality Index (DLQI)—a simple practical measure for routine clinical use. Clin Exp Dermatol. 1994;19:210–6.
Hongbo Y, Thomes CL, Harrison MA, et al. Translating the science of quality of life into practice: what do dermatology life quality index scores mean? J Invest Dermatol. 2005;125:659–64.
Kamudoni P, Mueller B, Salek MS. The development and validation of a disease-specific quality of life measure in hyperhidrosis: the Hyperhidrosis Quality of Life Index (HidroQOL©). Qual Life Res. 2015;24:1017–27.
Streiner DL, Norman GR, Cairney J. Health measurement scales: a practical guide to their development and use. 5th ed. Oxford: Oxford University Press; 2015.
Santiago-et-Sánchez-Mateos JL, Beà S, Fernández M, et al. Botulinum toxin type A for the preventive treatment of intertrigo in a patient with Darier’s disease and inguinal hyperhidrosis. Dermatol Surg. 2008;34:1733–7.
Kontochristopoulos G, Katsavou AN, Kalogirou O, et al. Botulinum toxin type A: an alternative symptomatic management of Darier’s disease. Dermatol Surg. 2007;33:882–3.
Ossorio-García L, Collantes-Rodríguez C, Villegas-Romero I, Linares-Barrios M. Vegetating Darier disease treated with botulinum toxin. JAMA Dermatol. 2018;154:106–8.
Testa MA, Simonson DC. Assessment of Quality-of-Life outcomes. N Engl J Med. 1996;334:835–40.
Monheit GD, Pickett A. AbobotulinumtoxinA: a 25-year history. Aesthet Surg J. 2017;37:S4–11.
The patients in this manuscript have given written informed consent to publication of their case details. We are extremely grateful to the patients who participated in the study and to the patient’s association for its support.
Institutional funding—Institutional tender.
Consent for publication
The patients in this manuscript have given written informed consent to publication of their case details.
Ethics approval and consent to participate
The study protocol was approved the 25, February 2015 by the institutional ethics committee (Comité de Protection des Personnes—Aquitaine, Bordeaux, France) and the 31, July 2015 by the French national health authority, ANSM (Agence Nationale de Sécurité du Médicament et des produits de santé). The implementation agreement was signed by the sponsor the 31, August 2015. The study was conducted in accordance with the principles of the Declaration of Helsinki 1975, revised 1983, and registered the 2, June 2015 under the European database Eudract identifier 2015-000379-29 and the 25, May 2016 under the ClinicalTrials.gov identifier NCT02782702 (https://clinicaltrials.gov/ct2/show/NCT02782702?cond=NCT02782702&draw=2&rank=1). Written consent was obtained from all patients.
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Additional file 1.
Additional file 2.
The 5-points photographic scale to evaluate HHD and DD clinical severity.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
About this article
Cite this article
Dreyfus, I., Maza, A., Rodriguez, L. et al. Botulinum toxin injections as an effective treatment for patients with intertriginous Hailey-Hailey or Darier disease: an open-label 6-month pilot interventional study. Orphanet J Rare Dis 16, 93 (2021). https://doi.org/10.1186/s13023-021-01710-x
- Botulinum toxin
- Hailey hailey disease
- Darier disease
- Quality of life