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Pharmaceutical compounding of orphan active ingredients in Belgium: how community and hospital pharmacists can address the needs of patients with rare diseases

Abstract

Background

Pharmaceutical compounding of orphan active ingredients can offer cost-effective treatment to patients when no other drug product is available for a rare disease or during periods of drug product shortages. Additionally, it allows customized therapy for patients with rare diseases. However, standardized compounding formulas and procedures, and monographs are required to ensure the patients’ safety.

Results

Standardized formulas and compounding procedures were developed for seven orphan active ingredients (L-arginine, sodium benzoate, sodium phenylbutyrate, L-carnitine, chenodesoxycholic acid, primaquine phosphate, pyridoxal phosphate) and one non-orphan molecule (sodium perchlorate) regularly compounded by hospital pharmacists for extemporaneous use. The stability of these formulations was evaluated over 3 months at refrigerated (5 °C) and standard storage conditions (25 °C/60%RH) using HPLC-based assays and a suitable shelf life was assigned to the formulations. Additionally, suitable analytical methods for quality control of formulations of pyridoxal phosphate and sodium perchlorate were developed as monographs for these components were not available in the European Pharmacopeia or United States Pharmacopeia.

Conclusions

Availability of compounding formulas and protocols, as well as stability information, for orphan active ingredients can improve patients’ access to treatment for rare diseases. Such data were collected for seven orphan active ingredients to treat patients with rare diseases when no other treatment is available. More efforts are needed to develop standardized formulas and compounding procedures for additional orphan active ingredients whose clinical efficacy is well-known but which are not available as products with a marketing authorization. Additionally, a legal framework at EU level is required to enable the full potential of pharmaceutical compounding for orphan active ingredients.

Background

In the European Union (EU), rare diseases are defined as life-threatening or chronically debilitating conditions that affect no more than five in 10 000 patients [1]. It is estimated that 5000 to 8000 rare diseases exist worldwide, while approximately 250 new diseases are described annually [1,2,3]. Although their prevalence is low, rare diseases affect 27 to 36 million people in the EU [1, 2]. Orphan medicinal products (OMPs) are defined by the EU as drug products intended for a condition for which there exists no authorized satisfactory method of diagnosis, prevention or treatment or, if so, that it will be of significant benefit to those affected by the condition [1]. The development of and patient access to OMPs is therefore an important challenge for public health policymakers. However, the challenges associated with OMPs are manifold and are mainly related to lack of research and development of OMPs and patient access to OMPs.

The low number of patients with a particular rare disease can limit OMP development due to the risk for pharmaceutical companies to achieve sufficient return on their investment and the difficulty in recruiting sufficient numbers of patients to prove statistically significant effects in clinical trials [3]. The economical aspect was addressed by the Orphan Drug Act in the United States in 1983 and the adoption of the European Commission Regulation Number 141/2000 and 847/2000 in 2000, offering economic and regulatory incentives [3]. In the EU, these include protocol assistance, 10-year market exclusivity and fee waivers for regulatory procedures for designated products for companies investing in the research and development of OMPs [3]. Additionally, the US Food and Drug Administration (FDA) and the European Medicine Agency (EMA) have enabled a common EMA-FDA application for orphan designation. Next to OMP development, patient access is essential but is often limited by high costs and lack of reimbursement [4]. As pricing and reimbursement of OMPs are EU member state responsibilities, patient access varies within the EU. The EU Commission therefore set up a working party to exchange knowledge between member states on scientific assessment of the clinical added value of orphan medicines that can facilitate national pricing and reimbursement decisions [5].

An increasing number of OMP designations and authorizations have been reported in the EU since 2000 [3, 6]. From 2000 to 2017 the EMA reported 1952 active OMP designations (i.e. designation of the status of OMP to a drug product) while 142 products received market authorization (i.e. a license to market a drug product) [6]. This is a success considering that there was almost no development of OMPs in the EU prior to 2000 [3, 6]. However, this also signifies that for less than 2% of rare diseases, authorized medicinal products are available in the EU and that continued efforts from the EU and its member states are required [2]. Therefore, the EU Commission and Council obligated their member states to develop dedicated national strategies to pursue a comprehensive and integrated approach to the delivery of health and social care for rare disease patients [1, 5].

In Belgium, the Fund for Rare Diseases and Orphan Drugs drafted a report including 42 recommendations and proposals for a national plan for rare diseases [7]. These recommendations are related to (1) expertise and multi-disciplinarity, (2) collaboration and networking, (3) knowledge, information and awareness, (4) equity in access, (5) governance and sustainability [7]. A measure to improve equity in access comprises creation of a legal framework to use orphan active ingredients (OAIs) in compounded preparations. Simultaneously, compounding of OAIs could offer opportunities to cut costs in the health care budget. Based on the latest available figures, the Belgian social security services reimbursed an increasing number of OMPs from 2009 to 2016, with expenditures rising from around 150 million euro in 2009 up to more than 350 million euro in 2016, which accounts for a rise from around 4% to up to more than 8% of the budget spent on medicine reimbursement [8]. Begin 2018, 149 OMPs had a marketing authorization and 61% of these were reimbursed [8]. Some OMPs were not reimbursed because alternatives exist that are more cost-effective or due to budgetary restrictions, while for others no reimbursement was applied for or the application procedure is still ongoing [8].

Some well-known OAIs for treatment of orphan diseases are not available to patients as products with a marketing authorization. Consequently, off-label use is widespread for treatment of orphan diseases [9]. However, compounding of OAIs could address these patients’ needs. Additionally, compounding of OAIs can address the needs of pediatric or geriatric patients, offering dosing flexibility, and of patients with hypersensitivity, intolerances and allergies to specific excipients of authorized drug products. However, three main obstacles must be overcome to use OAIs in compounded preparations. Firstly, compounding of OAIs should be performed by pharmacists applying standardized compounding procedures and formulas, with knowledge of the stability, in order to assure patient safety [10]. Secondly, monographs for pharmaceutical analysis of OAIs are often not available, hence suitable analytical methods must be developed for quality control of these compounds, reflected in their certificates of analysis, as well as for the compounded formulations. Thirdly, there is a lack of EU-uniform legal framework to use OAIs in compounded preparations. Despite these hurdles, some OAIs are currently used in enteral and parenteral preparations compounded in community and hospital pharmacists in Belgium and other countries when no other treatment is available for patients with rare diseases [11,12,13,14]. Nevertheless, to ensure the patients’ safety and to improve the equity in access, efforts are needed to develop standardized compounding procedures and formulas, to develop quality monographs (if none are available) and to create a legal framework.

The Laboratory of Drug Quality and Registration and the Laboratory of Pharmaceutical Technology at Ghent University took the initiative to develop formulas and standard compounding procedures and to develop suitable analytical methods for quality control of the compounded formulations in order to promote pharmaceutical compounding of OAIs by hospital and community pharmacists. These formulas and procedures are outlined in the current paper.

Methods

Materials

An overview of the OAIs, excipients and packaging used in the formulations is given in Table 1.

Table 1 Overview of the OAIs, excipients and packaging used in the compounded formulations

Methods

Selection of orphan active ingredients

A list of OAIs for use in galenic preparations and showing an unmet medical need was composed by an expert panel, consisting of 20 hospital pharmacists, and approved by the Belgian National Formulary commission. These OAIs were scored based on (A) the prevalence of the corresponding rare disease, (B) severity of the disease, (C) degree of available clinical and therapeutical evidence, (D) price of the OAI and (E) ease of compounding. Scores from 1 (for questionable/low) to 3 (for evident/high) were assigned to these criteria. Next, a weighing factor was assigned to the criteria depending on their importance (Table 2). Finally, the total score was calculated by summing the scores of the individual criteria multiplied with the corresponding weighing factor (Table 2). All active ingredients included in current study obtained orphan designation by EMA and/or FDA [15, 16].

Table 2 Priority list of the OAIs

Development of compounding formulations

The OAIs were compounded as liquid (solutions) or solid (capsules) formulations intended for oral administration. The choice for a specific dosage form depended on the OAI dose, dosing frequency, patient population, solubility and sensitivity to air, heat and light. The OAI concentration in the liquid formulations and the OAI content per capsule was based on scientific literature and the expert panel’s advice (Table 3).

Table 3 Overview of the OAIs included in current study

The preparation of the formulations was standardized following the best practices described in the Belgian National Formulary. Capsules were prepared using a mannitol/colloidal silicon dioxide mixture (99.5/0.5 w/w%) as a non-hygroscopic, non-reducing filler. Capsule size number 2 or larger was used. Capsules were packaged in plastic opaque cups of 100 ml. Solutions were compounded by dissolving the AOI in a glass flask in 50 ml demineralized water containing 0.02% propylparahydroxybenzoate (PBA) and 0.08% methylparahydroxybenzoate (MBA). Next, at least 85 g sirupus simplex (consisting of 65.00 w/w% sucrose, 0.02% propylparahydroxybenzoate and 0.08% methylparahydroxybenzoate in deionized water) and 3 g aurantii amari epicarp et mesocarp tincture were added. Finally, the preparation was diluted with sirupus simplex up to a total volume of 100 ml. The solutions were filled into transparent glass flasks, except for the L-arginine solution which was filled into a brown glass flask to avoid degradation through light exposure [30].

Stability testing

Identification, assay, related substances (i.e. degradation products), mass uniformity (for capsules) and pH (for solutions) tests were conducted on the preparations immediately after preparation (T0), after 3 months storage at 5 °C (T3_5°C), after 1 (T1_25°C/60%RH) and 3 (T3_25°C/60%RH) months storage at 25 °C and 60% relative humidity (RH) in constant climate chambers (Pharma 500 l, Weiss Technik, Liedekerke, Belgium). For chenodesoxycholic acid capsules, no data was collected after 3 months but after 4 months storage. Maximal storage duration of 3 or 4 months was considered sufficient, as the compounded formulations are delivered to the patient immediately after preparation. Due to detection of degradation products in the arginine solutions, assay determination of MBA and PBA in the arginine solutions was performed after 1 and 2 months storage under two conditions: (1) in the fridge (5 °C) and (2) at 25 °C and 60% RH (no data was collected after 3 months storage as clear degradation of the parabens was already detected after 2 months). Additionally, all samples were visually inspected at all timepoints for color change and evaluated for odor.

All identification, assay and related substances (i.e. degradation products) tests, except for chenodesoxycholic acid and sodium perchlorate, were performed using in-house developed and validated high-performance liquid chromatography (HPLC) methods with ultraviolet (UV) spectroscopy detection. Identification, assay and related substances tests, of sodium perchlorate were performed using an in-house developed and validated UPLC method with mass spectrometry detection. The details of these analytical methods are given in Table 4. Identification and determination of the related substances, impurities and degradation products of chenodesoxycholic acid was performed by thin layer chromatography as described in its European Pharmacopeia (Ph. Eur.) monograph whereas assay of chenodesoxycholic acid itself was performed by acid-base titration using an aqueous sodium hydroxide solution [31].

Table 4 Overview of the applied analytical methods

An OAI was identified when its retention time was within ±0.5 min of the retention time of the OAI peak in a reference standard (the active ingredient used in the formulation) or when the principal spot on the thin layer chromatogram of the test solution was similar in position, color and size to the principal spot in the chromatogram obtained with the reference solution. For assay determination via HPLC, two reference solutions and two sample solutions were prepared independently and analyzed in duplicate. Standard curves of each component were constructed via linear regression of the peak area to the concentration and used to calculate the percentage label claim. The formulations complied to the assay test when the assay of the solutions and capsules was within a 90–110% label claim and 85–115% label claim interval, respectively. A reporting threshold of 0.1% label claim was applied for related substances (i.e. degradation products) according to the Ph. Eur. monograph on substances for pharmaceutical use, unless other limits were included in the specific Ph Eur. monograph of a OAI [32]. The mass uniformity of the capsules was evaluated at release and during stability testing according to the monograph for uniformity of mass of single dose preparations of the Ph. Eur. [33].

Results

Selection of orphan active ingredients

A priority list based on the scores assigned to the unlicensed OAIs defined by an expert panel of the Belgian National Formulary is given in Table 2. It was decided to develop standard compounding procedures for the OAIs with the five highest scores. Although amifampridine scored highest, no compounding procedure was developed as this is already available in the German National Formulary (Deutsche Artsenei Codex). Standardized formulas and compounding procedures were developed for sodium phenylbutyrate, pyridoxal phosphate, chenodesoxycholic acid and primaquine phosphate. Additionally, standardized formulas and compounding procedures were developed for three licensed OAIs which are frequently compounded in Belgian hospital pharmacies but whose stability was not investigated up to now: L-arginine, L-carnitine and sodium benzoate. Sodium perchlorate, which is not an OAI but an unlicensed molecule, was also included in the study based on the input of the expert panel as sodium perchlorate is frequently compounded by hospital pharmacists for extemporaneous use prior and after radiological examination of the thyroid, although stability data was lacking up to now.

An overview of the compounded OAIs, their recommended dose and the corresponding developed formulation is presented in Table 3. Additionally, the rare diseases and their prevalence for the corresponding OAIs are included in Table 3.

Next to the standardized formulas and compounding procedures, suitable analytical methods were developed for quality control of pyridoxal phosphate and sodium perchlorate formulations as no monographs for these components were available in the Ph. Eur. or USP. Details of these tests are included in Table 4.

Stability testing

The suitability of the developed formulas and compounding procedures was evaluated via stability testing to investigate how long the extemporaneously produced preparations can be stored and which storage conditions should be applied to ensure their activity. All formulations complied with the mass uniformity test, identification and related substances tests at release. Additionally, all formulations complied with the mass uniformity and identification tests during stability testing. An overview of the assay results at the investigated time points is presented in Table 5. The L-carnitine, sodium benzoate and sodium phenylbutyrate solutions and chenodesoxycholic acid, pyridoxal phosphate and primaquine phosphate capsules were stable over 3 months under all storage conditions. All formulations, except the L-carnitine and L-arginine solutions, complied to the specifications for related substances (i.e. degradation products). A degradation peak (RRT 0.89) exceeding the reporting threshold (0.10% label claim) was detected in the L-carnitine solutions after 1 (0.24% label claim) and 2 months (0.17% label claim) only (and not after 3 months). However, as the assay of L-carnitine was stable over 3 months and as the detected degradation peak was a broad HPLC-peak, which is atypical for a well-identified degradant, it was assumed that the observed degradation peak was due to degradation of the aroma, aurantii amari epicarp et mesocarp, which is a complex mixture [34, 35]. Therefore, a shelf life of 3 months was assigned to the L-carnitine solutions. L-arginine was stable over the tested time period, but degradation of PBA and MBA in the L-arginine solution was observed after 1 month storage at both refrigerated and 25 °C/60% RH conditions in a brown glass flask. Paraben degradation was attributed to hydrolysis in alkaline environment, as paraben hydrolysis is commonly observed at pH values exceeding 7.0, and as the pH of the L-arginine solutions was 10.5. An overview of the paraben decrease over time in function of the storage conditions is given in Fig. 1. Faster degradation was observed for MBA compared to PBA which was attributed to the stronger resistance to hydrolysis observed with an increase in alkyl chain length of parabens [36]. Assuming linear degradation kinetics, based on the collected data points, the arginine solution could be stored for maximum 9 days under refrigerated conditions. Therefore a shelf life of 1 week under refrigerated conditions was recommended (Table 5).

Table 5 Overview of the shelf life and assay stability results
Fig. 1
figure1

Label claim% of MBA and PBA in the L-arginine solution in function of time. Label claim % of MBA (grey) and PBA (black) included in the L-arginine solution after storage at 5 °C () or 25 °C and 60%RH (■)

Discussion

The developed formulas and compounding procedures for the selected OAIs can offer patient access to treatment of rare diseases when no commercial orphan medicinal product (OMP) is on the market, provided a legal framework is created. Additionally, similar work should be performed for other OAIs that are not commercially available to address the needs of patients with rare diseases. Several OAIs were suggested in the report of the Belgian Fund for Rare Diseases and Orphan Drugs or by the expert panel including d,I-3-sodiumhydroxybutyrate, amiloride, bi-myconase, I-citrulline, CoEnzyme Q10, diphencyprone, d-mannose, d-ribose, fenfluramine, glycine, hydroxobalamine, oxybutinine, squaric acid dibuty ester, potassium citrate, propranolol and sodium thiosulphate [7].

Next to increasing patient access, compounding of OAIs can also be an interesting way to cut costs in the portion of the health care budget dedicated to rare diseases. Although some OMPs are novel or complex new chemical entities developed at high research and development (R&D) costs, such high R&D costs are not applicable to others as either evidence of their efficacy has already been published in literature prior to market authorization, or because the drug product was already developed for another diseases, decreasing to some extend the costs in the quality and preclinical field [37, 38]. In this context, Simoens et al. compared the price of commercially manufactured OMPs to compounding costs in a community pharmacy for a selection of orphan drugs on the Belgian market in 2011. The price of commercially manufactured OMPs exceeded 2- to 148-fold the compounding costs for the studied OMP [38].

While standardized formulas and compounding procedures were developed for seven OAIs in current study, monographs and compounding procedures for other OAIs are included in the German National Formulary (e.g. cysteamine, polyhexamethylbiguanide, amifampridine), USP (e.g. mepacrine) or others. Dooms et al. listed national formularies and other reliable sources available worldwide to retrieve information on compounding of OAIs for rare diseases [10]. To realize all benefits of compounding OAIs, it is essential to create a legal framework for their use. Preferably, this should be organized at EU level by creation of an authority listing and certifying OAIs and issuing a formulary including standardized formulas and compounding procedures.

Conclusions

Compounded preparations of OAIs can improve patients’ access to treatment for rare diseases. However, they should be prepared in accordance to standardized formulas and compounding procedures and the quality of the OAI should be demonstrated by a certificate of analysis according to a specific monograph. In current study, such standardized formulas and compounding procedures were developed for seven OAIs to treat patients with rare diseases when no other treatment is available. More efforts are needed to develop standardized formulas and compounding procedures for additional OAIs whose clinical efficacy is well-known but are not available yet to patients due to lack of interest from the pharmaceutical industry to apply for market authorization as OMP. Additionally, a legal framework at EU level is required to enable the potential of pharmaceutical compounding for OAIs.

Availability of data and materials

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

Abbreviations

EMA:

European Medicine Agency

EU:

European Union

FDA:

Food and Drug Administration

HPLC:

High-performance liquid chromatography

MBA:

Methylparahydroxybenzoate

OAI:

Orphan Active Ingredient

OMP:

Orphan Medicinal Product

PBA:

Propylparahydroxybenzoate

Ph. Eur.:

European Pharmacopeia

R&D:

Research and development

USP:

United States Pharmacopeia

UV:

Ultraviolet

References

  1. 1.

    Council Recommendation of 8 June 2009 on an action in the field of rare diseases. Official Journal of the European Union 2009/C 151/02 of 8 June 2009. https://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:C:2009:151:0007:0010:EN:PDF.

  2. 2.

    Inventory of Union and Member State incentives to support research into, and the development and availability of, orphan medicinal products - Final state of Play 2015. https://ec.europa.eu/health//sites/health/files/files/orphanmp/doc/orphan_inv_report_20160126.pdf. Accessed 26 Mar 2019.

  3. 3.

    The committee for orphan medicinal products and the European medicines agency scientific secretariat. European regulation on orphan medicinal products: 10 years of experience and future perspectives. Nat Rev Drug Discov. 2011;10:341–9.

  4. 4.

    Denis A, Mergaert L, Fostier C, Cleemput I, Simoens S. Weesgeneesmiddelen: een Belgische en Europese analyse. Farmaceutisch tijdschrift Voor België. 2009;4:109–15.

  5. 5.

    Communication from the commission to the European Parliament, the Council, the European Economic and Social Committee and the Committee of the regions, on rare diseases: Europe’s challenges. https://publications.europa.eu/en/publication-detail/-/publication/c8a042d8-ffb9-4b01-9c91-c1497a2b3fd7/language-en. Accessed 26 Mar 2019.

  6. 6.

    EMA. Orphan Medicines Figures 2000–2017. https://www.ema.europa.eu/documents/other/orphan-medicines-figures-2000-2017_en.pdf. Accessed 31 Dec 2018.

  7. 7.

    Fonds zeldzame ziekten en weesgeneesmiddelen. Aanbevelingen en voorstellen tot maatregelen voor een Belgisch plan voor zeldzame ziekten. https://www.zorg-en-gezondheid.be/sites/default/files/atoms/files/Plan_ZZ_aanbevelingen_KBS.pdf. Accessed 26 Mar 2019.

  8. 8.

    Directie Farmaceutisch Beleid – Dienst voor Geneeskundige Verzorging RIZIV. Monitoring of reimbursement significant expenses report MORSE 2018. https://www.riziv.fgov.be/SiteCollectionDocuments/morse-rapport-2016.pdf. Accessed 31 Dec 2018.

  9. 9.

    STAMP Commission Expert Group. Off-label use of medicinal products, report of 14 March 2017. https://ec.europa.eu/health/sites/health/files/files/committee/stamp/stamp6_off_label_use_background.pdf. Accessed 11 July 2018.

  10. 10.

    Dooms M, Carvalho M. Compounded medication for patients with rare diseases. Orphanet J Rare Dis. 2018;13:1–8.

  11. 11.

    Dooms M, Pincé H, Simoens S. Do we need authorized orphan drugs when compounded medications are available? J Clin Pharm Ther. 2013;38:1–2.

  12. 12.

    Dooms M. Weesgeneesmiddelen. Farmaceutisch Tijdschrift Voor België. 2009;1:9–13.

  13. 13.

    Minghetti P, Giudici EM, Montanari L. A proposal to improve the supply of orphan drugs. Pharmacol Res. 2000;42:33–7.

  14. 14.

    Peila E, Fogliano MR, Crosasso P, Mosso B, Chiappetta MR, Viterbo ML, Mazengo M, Stecca S, Baldovino S, Roccatello D. Extemporaneous orphan drugs and preparations for rare diseases: organization and management of an inter-regional network. Eur J Hosp Pharm. 2011;17:40–5.

  15. 15.

    The portal for rare diseases and orphan drugs. http://www.orpha.net/consor/cgi-bin/Disease.php?lng=EN. Accessed 26 Mar 2019.

  16. 16.

    U.S. Food and drug administration Search orphan drug designations and approvals. https://www.accessdata.fda.gov/scripts/opdlisting/oopd/. Accessed 12 June 2019.

  17. 17.

    Häberle J, Boddaert N, Burlina A, Chakrapani A, Dixon M, Huemer M, Karall D, Martinelli D, Sanjurjo Crespo P, Santer R, Servais A, Valayannopoulos V, Lindner M, Rubio V, Dionisi-Vici C. Suggested guidelines for the diagnosis and management of urea cycle disorders. Orphanet J Rare Dis. 2012;7:32–62.

  18. 18.

    Matern D, Rinaldo P. Medium-Chain Acyl-Coenzyme A Dehydrogenase Deficiency. In: Adam MP, editor. Gene Reviews. Seattle; 2015. https://www.ncbi.nlm.nih.gov/books/NBK1424/. Accessed 26 Mar 2019.

  19. 19.

    El-Hattab AW. Systemic Primary Carnitine Deficiency. In: Adam MP, editor. Gene Reviews. Seattle; 2016. https://www.ncbi.nlm.nih.gov/books/NBK84551/. Accessed 26 Mar 2019.

  20. 20.

    Van Hove J, Coughlin C, Scharer G. Glycine Encephalopathy. In: Adam MP, editor. Gene Reviews. Seattle; 2013. https://www.ncbi.nlm.nih.gov/books/NBK1357/. Accessed 26 Mar 2019.

  21. 21.

    Federico A, Dotti MT, Gallus GN. Cerebrotendinous Xanthomatosis. In: Adam MP, editor. Gene Reviews. Seattle; 2016. https://www.ncbi.nlm.nih.gov/books/NBK1409/. Accessed 26 Mar 2019.

  22. 22.

    Nie S, Chen G, Cao X, Zhang Y. Cerebrotendinous xanthomatosis: a comprehensive review of pathogenesis, clinical manifestations, diagnosis and management. Orphanet J Rare Dis. 2014;9:1–11.

  23. 23.

    WHO. Essential Medicines and health products information portal. http://apps.who.int/medicinedocs/en/d/Jh2922e/2.5.4.html#Jh2922e.2.5.4. Accessed 26 Mar 2019.

  24. 24.

    WHO. Essential Medicines and health products information portal. http://apps.who.int/medicinedocs/en/d/Js2215e/9.21.html#Js2215e.9.21. Accessed 26 Mar 2019.

  25. 25.

    Ashley EA, Recht J, White NJ. Primaquine: the risks and the benefits. Malar J. 2014;13:418–25.

  26. 26.

    Fernando D, Rodrigo C, Rajapakse S. Primaquine in vivax malaria: an update and review on management issues. Malar J. 2011;10:351–63.

  27. 27.

    Serrano C, Joret P, Siorat V, Vaconsin P, Abarou T, Storme T. Development of Pyridoxal-5-phosphate hard capsules for paediatric use. Eur J Hosp Pharm. 2013;20:A72.

  28. 28.

    Hoffmann GF, Schmitt B, Windfuhr M, Wagner N, Strehl H, Gagci S, Franz AR, Mills PB, Clayton PT, Baumgartner MR, Steinmann B, Bast T, Wolf NI, Zschocke J. Pyridoxal-5-phosphate may be curative in early-onset epileptic encephalopathy. J Inherited Metab Dis. 2007;30:96–9.

  29. 29.

    BCFI. http://www.bcfi.be/nl/articles/891?folia=839&matches=. Accessed 26 Mar 2019.

  30. 30.

    European Pharmacopeia, Monograph 0806 on L-arginine. 2015.

  31. 31.

    European Pharmacopeia, Monograph 1189 on chenodesoxycholic acid.

  32. 32.

    European Pharmacopeia, Monograph 2034 on substances for pharmaceutical use.

  33. 33.

    European Pharmacopeia, Monograph 2.9.6 on uniformity of mass of single dose preparations.

  34. 34.

    European Pharmacopeia, Monograph 1603 on bitter-orange epicarp and mesocarp.

  35. 35.

    European Pharmacopeia, Monograph 1604 on bitter-orange epicarp and mesocarp tincture.

  36. 36.

    Soni MG, Carabin IG, Burdock GA. Safety assessment of esters of p-hydroxybenzoic acid (parabens). Food Chem Toxicol. 2005;43:985–1015.

  37. 37.

    Dooms M. Weesgeneesmiddelen: zes spijtige misverstanden en één harde waarheid. Antwerps Farmaceutisch Tijdschrift. 2009;4:34–6.

  38. 38.

    Simoens S, Cassiman D, Picavet E, Dooms M. Are some orphan drugs for rare diseases too expensive? Drugs Ther Perspect. 2011;27:24–6.

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Acknowledgements

We thank M. D’Hondt, L. Tavernier, M. Verbeken and F. Verbeke for help in the analytical development and stability study.

Funding

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Author information

VV, EP, IVT, KB, EW, BDS, JPR and CV designed the study. VV and EP compounded the preparations. EW and BDS developed validated analytical methods for analysis of the preparations and interpreted the analytical data. VV wrote the manuscript. All authors read and approved the final manuscript.

Correspondence to V. Vanhoorne.

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Keywords

  • Rare diseases
  • Orphan active ingredients
  • Pharmaceutical compounding