Whole lung lavage therapy for pulmonary alveolar proteinosis: a global survey of current practices and procedures
- Ilaria Campo†1, 12Email authorView ORCID ID profile,
- Maurizio Luisetti†1,
- Matthias Griese2,
- Bruce C. Trapnell3,
- Francesco Bonella4,
- Jan Grutters5,
- Koh Nakata6,
- Coline H. M. Van Moorsel5,
- Ulrich Costabel4,
- Vincent Cottin7,
- Toshio Ichiwata8,
- Yoshikazu Inoue9,
- Antonio Braschi10,
- Giacomo Bonizzoni1,
- Giorgio A. Iotti10,
- Carmine Tinelli11,
- Giuseppe Rodi10 and
- for the WLL International Study Group
© The Author(s). 2016
Received: 23 April 2016
Accepted: 3 August 2016
Published: 31 August 2016
Whole lung lavage (WLL) is the current standard of care treatment for patients affected by pulmonary alveolar proteinosis (PAP). However, WLL is not standardized and international consensus documents are lacking.
Our aim was to obtain a factual portrayal of WLL as currently practiced with respect to the procedure, indications for its use, evaluation of therapeutic benefit and complication rate.
A clinical practice survey was conducted globally by means of a questionnaire and included 27 centers performing WLL in pediatric and/or adult PAP patients.
We collected completed questionnaires from 20 centres in 14 countries, practicing WLL in adults and 10 centers in 6 countries, practicing WLL in pediatric patients.
WLL is almost universally performed under general anesthesia, with a double-lumen endobronchial tube in two consecutive sessions, with an interval of 1–2 weeks between sessions in approximately 50 % of centres. The use of saline warmed to 37 °C, drainage of lung lavage fluid by gravity and indications for WLL therapy in PAP were homogenous across centres.
There was great variation in the choice of the first lung to be lavaged: 50 % of centres based the choice on imaging, whereas 50 % always started with the left lung. The choice of position was also widely discordant; the supine position was chosen by 50 % of centres. Other aspects varied significantly among centres including contraindications, methods and timing of follow up, use of chest percussion, timing of extubation following WLL and lung isolation and lavage methods for small children. The amount of fluid used to perform the WLL is a critical aspect. Whilst a general consensus exists on the single aliquot of fluid for lavage (around 800 ml of warm saline, in adults) great variability exists in the total volume instilled per lung, ranging from 5 to 40 liters, with an average of 15.4 liters/lung.
This international survey found that WLL is safe and effective as therapy for PAP. However these results also indicate that standardization of the procedure is required; the present survey represents the a first step toward building such a document.
Whole lung lavage (WLL) is a therapeutic procedure  used to treat pulmonary alveolar proteinosis (PAP), a rare syndrome occurring in a heterogeneous group of lung diseases characterized by accumulation of lipoproteinaceous material in the alveoli, whicht impairs oxygen uptake and causes hypoxemic respiratory failure [2, 3]. Therapeutic efficacy derives from removal of the accumulated lipoproteinaceous material – primarily surfactant and necrotic cell debris – by physically ‘washing’ the alveoli with saline. WLL is usually performed under general anesthesia with lung separation obtained by a double-lumen endobronchial tube. While mechanical ventilation is maintained in one lung, the contralateral lung is repeatedly filled with saline and then drained by gravity. Typically, the lavage is accompanied by chest percussion to emulsify the surfactant sediment, and is continued until the lavage fluid becomes clear, usually judged by visual inspection.
Presently applied WLL procedures are based on the first description by Juan Ramirez Rivera in 1963 , but several centres have introduced modifications with the aim of improving the original method . Although widely considered as the standard-of-care for autoimmune PAP , the WLL procedure, indications for its use, and the criteria to measure outcome have not been standardized among centres. Nor has the therapeutic effectiveness been compared for different secondary PAP syndromes. Further, centre-specific indications have never been compared, integrated to optimize or standardize WLL, or systematically disseminated. Moreover training on how to perform WLL involves an apprenticeship or in many cases is self taught; which obviously leads to additional variations among centres.
To begin laying the foundation for a consensus and experience-based, best-practice standardization of WLL, physicians performing WLL were surveyed by questionnaire on a global scale. Results concern the practice of WLL in adult and paediatric patients and include information on the procedure itself, local modifications, indications for use, contraindications, procedural monitoring, criteria to measure outcomes and complication rates.
WLL questionnaire development
This study was conducted as part of the e-RARE 2009 EuPAPnet study (http://www.alveolarproteinosis.eu/). An international ad hoc committee of physicians performing WLL and/or caring for PAP patients developed the survey questionnaire, which includes sections for physicians only performing WLL in either adult (≥18 years-old) or paediatric (<18 years-old) patients; only lobar or segmental bronchoscopic lavage (LSBL) in adult and/or paediatric patients; or both WLL and LSBL. The questionnaire is available in the Additional file 1.
The study cohort included physicians performing therapeutic lung lavage. Potential partecipants were identified either through a PubMed search (http://www.ncbi.nlm.nih.gov/pubmed) using the search terms ‘pulmonary proteinosis’, ‘alveolar proteinosis’, ‘alveolar phospholipidosis’, ‘lung lavage’, and ‘whole lung lavage’; or invited by an announcement placed in the European Respiratory Journal  or directly contacted: i.e., members of the international paediatric interstitial lung disease network; and acquaintances of steering committee members. Potential respondents were contacted by e-mail, interested individuals received the questionnaire by email, and all completed questionnaires that were returned were included in the analysis. All respondents gave written authorization for their responses to be included in the study.
Numeric variables were evaluated for normality with the Shapiro-Wilk test and expressed as the mean +/− SD or median +/− the interquartile range (IQR) as appropriate. Comparisons were made by one-way ANOVA with the Bonferroni correction. Categorical data were summarized numerically or expressed as a percentage and compared using the chi square or Fisher’s exact test as appropriate. Correlations among continuous variables were made using Pearson’s correlation coefficient. All comparisons were two-sided. P values of <0.05 were considered statistically significant. Analyses were performed using STATA, version 13.1 (Stata Corporation, College Station, Texas, USA). Three categories for the number of times WLL was conducted in a single patient (WLL < 2, ≥2 WLL <3, WLL >3) were utilized in analyzing results related to clinical outcomes.
Data were considered in a two-stage IPD meta-analysis, in order to correct the unavailability of single patient data. In the first stage, each individual study was analyzed as described in the meta-analysis protocol or analysis plan. As a second step, the results, or summary statistics, of each of these individual study analyses were combined to provide a pooled estimate of effect, as for a conventional systematic review.
Centres participating in the survey
Sheffield Children’s Hospital, UK
Royal Brompton Hospital, London, UK
Sheba Medical Centre, Tel-Hashomer, Tel Aviv U., Israel
Children’s Hospital Boston, USA
Kinderklinik und Kinderpoliklinik im, U. of Munich, Germany
Cincinnati Children’s Hospital, USA
Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
University of North Carolina at Chapel Hill, USA
Hacettepe University, Ankara, Turkey
U. Witwatersrand, Johannesburg, South Africa
Pavlov State Medical University, St. Petersburg, Russian Federation
NHO Kinki-Chuo Chest Medical Centre, Osaka, Japan
U. Medical College, Hangzhou, Zhejiang, China
Lungenclinic Grosshandorf, Germany
U. Hospital, Olomouc, Czech Republic
Asklepios-Fachkliniken München Gauting, Germany
Helsinki University Central Hospital, Finland
Dept. of Respiratory Medicine, Cork University Hospital, Ireland
Care Medicine Thoraxklinik, Heidelberg, Germany
Serviço de Pneumologia- Hospital São João-Porto, Portugal
Ruhrlandklinik-University of Duisburg Essen, Germany
Pulmonary Division, University Hospital, Zurich, Switzerland
Hopital Louis Pradel, Lyon, France
Tokyo Medical U. Hachioji Medical Centre, Japan
Kantonsspidal Aarau, Switzerland
St. Antonius Hospital Nieuwegein, The Netherlands
Kempten-Oberallgäu Hospital, Immenstadt, Germany
WLL in Adult Patients
Indications and contraindications for WLL therapy
Indications for WLL therapy
Indications for WLL
% of centres
Unspecified decline in lung function
Decline in resting PaO2
Chest X-ray or CT
Decline in DLCO
Decline in FVC
Decline in SpO2
Three centres also reported using WLL for indications other than PAP, including: accidental inhalation of activated charcoal, alveolar hemorrhage, silicoproteinosis, lipoid pneumonia, silicosis or cryptogenic fibrosing alveolitis (CFA).
Contraindications to WLL were applied by six centres and included severe cardiovascular disease, heart failure, sepsis, significant lung infection and end-stage pulmonary fibrosis.
Interval between treatment of right and left lungs
Most (17/20) centres performed WLL in separate sessions on each lung for a given patient. The time separating WLL therapy for each lung was 2.9 ± 1.18 weeks and varied from 24 h at one centre to 6–12 weeks at another centre. Only one centre routinely treated both lungs in one session, while two other centres treated lungs in one or two sequential sessions depending on patient tolerance.
Number of WLL treatments Per patient required in autoimmune PAP
Because the clinical course of autoimmune PAP is variable, we evaluated whether the experience of the centre (number of years in activity and number of WLL procedures performed annually) and the volumes used for WLL might relate to clinical outcome. We found no correlation in these parameters (χ 2 = 0.1238 and 0.4905, respectively) suggesting that neither the experience level or volume of WLL fluid used were significantly correlated with clinical outcome.
Complications in WLL
Complications in WLL
Frequency (%)a, b
Prolonged mechanical ventilation
Transient cardiac ischemia
Follow up after lung lavage therapy
All centres reported performing a short-term assessment (chest x-ray and functional assessment) after WLL over a period that ranged from 2 h to 2 weeks. Some (9/20) centres also performed a follow up chest computed tomogram (CT) scan at a variable time ranging from 7 days to 6 months. All centres reported performing medium- to long-term follow up using conventional radiological and functional parameters including chest CT scan (12/20 centres – routinely at 8, and on an ‘as indicated basis’ at 4 months). Six centres performed follow up biomarker evaluations including serum LDH, GM-CSF autoantibody levels and less commonly SPA, SPD, KL-6, CEA, Cyfra 21–1. Two centres followed up WLL procedures by using a quality of life questionnaire.
Implementation of the WLL procedure
The lungs must be isolated during WLL so that one can be lavaged while the other is ventilated to maintain the necessary gas exchange. This is usually achieved by positioning a double-lumen endobronchial tube, which ensure an adequate seal to prevent fluid spillage from the treated lung into the ventilated lung. A left-sided double-lumen endobronchial tube is the most common choice in adults and larger children. The minimum size of the double-lumen tube is 26 Fr.
Parameters monitored during anesthesia/WLL
N° of centres (%)
20 (100 %)
18 (90 %)
Non invasive blood pressure
13 (65 %)
End tital CO2
13 (65 %)
Arterial catheter blood pressure
11 (55 %)
7 (35 %)
3 (15 %)
Blood gas analysis
3 (15 %)
1 (5 %)
The method used to decide which lung to treat first varied among centres. About half (12/20) used radiographic information to identify and treat the more severely affected lung first, which was based on visual inspection of the chest CT (9 centres), chest x-ray (2 centres), or a combination of chest x-ray and CT (1 centre). At six centres, the decision was independent of lung-specific severity: the left lung was always treated first, due to its smaller relative size. At about half of the centres (9/20), a procedure referred to as ‘lung atelectasis/degassing’ is performed prior to starting the lavage of each lung. Degassing of one lung is obtained by ventilation with 100 % oxygen followed by forced lung deflation with negative airway pressure and subsequent airway opening occlusion maintained for 10 to 15 min up to absorption atelectasis of the whole lung. Lung degassing is intended to help the lavage fluid reach the alveoli more easily and evenly.
The position of the patient during WLL varied considerably among centres: 12 (60 %) centres utilized a supine position; 6 (30 %) centres utilized a full lateral position at 90°; 2 centres utilized a moderate lateral position at 30° to 45° inclination. Of those utilizing a lateral position, all but one centre ventilated the non-dependent lung and lavaged the dependent lung. Only one centre uses a combination of full lateral position, dependent lung ventilation and non-dependent lung lavage, having observed better oxygenation due to better ventilation/perfusion matching, no problems with lavage spillover and more convenient access of the lavaged lung for manual chest percussion. Moreover, 7 centres indicated that patient positioning included the Trendelenburg position to improve lavage recovery and increase alveolar clearance.
Lavage fluid and administration
All Centres used saline warmed to 37 °C for WLL. Supplements used at some centres included (number of centres using the supplement): N-acetyl cysteine (1), aminophylline (1), hydrocortisone (1), sodium bicarbonate (2) and gaseous oxygen (1). One centre modified the filling-drainage cycle by adding manual ventilation of the lavaged lung half-way through the draining time, intended to improve the removal of material from the lung . All centres infused saline by gravity. One centre used a specified hydrostatic head of 30 cm relative to the mid-thorax.
Most (14/20) centres used chest percussion to emulsify the PAP sediment (~90 % lipid) in order to improve therapeutic efficiency. However, the method and timing varied greatly. Ten (50 %) centres utilized manual chest percussion and four (20 %) centres used mechanical percussion. The timing of chest percussion also varied with some centres utilizing it during the entirety of each infusion-drainage cycle, some during the drainage portion only, some only after filling.
Duration and termination
The duration of WLL for one lung varied from 2 to 6 h with an average of 6 +/− 1 h for both lungs. All centres terminated WLL when the drained fluid became markedly less cloudy than observed at the start of therapy; this was usually based on visual inspection of the gross appearance of the recovered fluid. Two centres also used an objective measure of protein content.
Lung Lavage in Paediatric Patients
Complications of WLL in children
Overall occurrence rate
As a first step in developing an evidence-based, best-practice approach to standardizing WLL therapy in PAP, we made the results of a global survey of physicians performing WLL in adults and children. Respondents included 20 centres in 14 countries performing WLL in adults and 10 centres in 6 countries performing WLL in paediatric patients. Some aspects of WLL were similar among centres, including the method for selecting the first lung to be treated, treatment of each lung at different sessions separated by several days to weeks, use of general anesthesia, a double-lumen endotracheal tube (in adults), saline warmed to 37 °C, and drainage of lung lavage fluid by gravity. Other aspects of the procedure varied significantly among centres, including indications for treatment, contraindications, methods and timing of the follow up evaluation, patient position during WLL, the volume of saline infused, use of chest percussion, timing of extubation after WLL and lung isolation and lavage methods for small children. The length of experience among centres varied widely, but the overall rate of serious, procedure-related complications was low. Limited number of diseases requiring WLL therapy, procedural variations and their relationship to differences in outcomes among patients and centres, all support the need for integrated data from multiple centres in order to develop a best-practices approach to standardizing WLL therapy.
Important findings of our WLL survey include: in spite of its clear therapeutic usefulness, it is not available at most medical centres; it is frequently taught by informal apprenticeship or is even self-taught and it varies among centres with respect to the procedure itself, indications for use, and assessment of benefit. We found 161 reports on the use of WLL therapy for PAP in the NCBI PubMed database including several technical descriptions [8–12]. There was a consensus on indications for WLL therapy in PAP, which included worsening of lung function/gas exchange (100 % of centres), followed by radiographic evidence of deterioration (79 %), and then symptomatic worsening (42 % of centres). Notwithstanding this consensus, the parameter used to determine each varied considerably among centres. Even though the majority of centres used ca. 800 ml of warmed saline for each single infusion during WLL, centres varied in their use and choice of additives, and the total volume of saline used to treat each lung. These differences have important implications for the conduct of clinical trials evaluating new therapeutic approaches.
Interestingly, only 65 % of the centres employed an observation period before performing the WLL. With the exception of rapidly progressive cases, this would be advisable as spontaneous improvement is possible. Even though, complete spontaneous remission, previously believed to occur in up to 30 % of cases , is now known not to exceed 10 % of cases [14, 15].
The absence of an association between variability in the clinical course among PAP patients (indicated by the different number of WLL treatments needed to obtain remission) and differences WLL practice among centres (volume of saline used, number of procedures performed annually, length of experience performing WLL) suggest that intrinsic disease characteristics predominate over technical differences and experience in applying WLL among centres. On the other hand, the low number of complications and absence of a correlation with experience status may be related to the fact that the centres filling out the questionnaire were all relatively experienced. In fact, several authors stated that they treated patients who were sent for a tertiary referral after a complicated WLL procedure in an inexperienced hospital.
Although WLL is an invasive procedure, it has been determined to be safe and associated with a low rate of procedure-related morbidity (~18 %, including, in order of decreasing frequency, fever, spillage of saline into the ventilated lung, and worsened hypoxemia). SLBL  is an alternative to WLL and is regarded as less invasive, but neither its therapeutic effectiveness, nor its rate of complications have been adequately studied and thus was not considered in the present study.
This study has some limitations, which interfere with the statistical analysis of the results, notably: the incomplete participation of physicians/centres who are known to practice WLL therapy. Thus, our results may not adequately reflect the practice of WLL at all centres or, potentially, important improvements made at centres not surveyed. Notwithstanding these limitations, these results provide the first useful data for developing consensus documents regarding technical implementation, indications, and evaluation of WLL therapy. For this reason, the questionnaire has been included in an Additional file 1 for use by other centres wishing to provide additional data regarding their centre’s practice of WLL. Even though few centres performing WLL in children were identified/included, one important problem identified with this survey was the lack of availability of double lumen endotracheal tubes of sufficiently small size for use in small children.
Our study (as well as the literature) provides data for the development of a consensus document and experience-based, best-practice standardization of WLL. We conclude that the assembly of an international task force on the conduct of WLL therapy in adults and children would be helpful in this regard.
CEA, carcinoembryonic antigen; CFA, cryptogenic fibrosing alveolitis; CT, computed tomogram; Cyfra 21–1, cytokeratin 19 fragment; DLCO, carbon monoxide diffusing capacity; FVC, forced vital capacity; GM-CSF, granulocyte-macrophage colony-stimulating factor; ICU, intensive care unit; IQR, interquartile range; KL-6, Krebs von den Lungen 6; LDH, lactate dehydrogenase; LSBL, lobar or segmental bronchoscopic lavage; NCBI, National Center for Biotechnology Information; PAP, pulmonary alveolar proteinosis; SPA, surfactant protein A; SPD, surfactant protein D; SpO2, resting oxygen saturation; WLL, whole lung lavage
This project was started by the late Dr. Maurizio Luisetti (1953 to 2014) whose passion for research and advocacy for people affected by rare diseases, including PAP, and recognition of the need and importance of optimizing and standardizing the practice of WLL, resulted in this project. The manuscript was completed by his collaborators and friends who want to honour his memory. We thank all of the physicians at centres performing WLL for participating in the survey.
Members of the Whole lavage International Study Group
Arai Toru, Department of Diffuse Lung Diseases and Respiratory Failure, Clinical Research Centre, National Hospital Organization Kinki-Chuo Chest Medical Centre, Osaka, Japan
Bazhanov Andrey A., Pulmonology Department of Pavlov State Medical University, St. Petersburg, Russian Federation
Ben-Dov Issahar, Pulmonary Institue’ Sheba Medicak Centre, Tel-Hashomer, Tel Aviv University, Sackler Med School, Israel
Bonella Francesco, Department of Pneumology/Allergy, Ruhrlandklinik-University of Duisburg Essen, Germany
Braschi Antonio, Department of Anaesthesiology and Intensive Care Unit, Fondazione IRCCS Policlinico San Matteo, University of Pavia, Pavia, Italy
Casey Alicia, Children’s Hospital Boston, Division of Respiratory Diseases, Boston, MA, USA
Costabel Ulrich, Department of Pneumology/Allergy, Ruhrlandklinik-University of Duisburg Essen, Germany
Cottin Vincent, National Reference centre for rare pulmonary disease, Hopital Louis Pradel, Lyon, France
Dogru Deniz, Hacettepe University, Faculty of Medicine, Paediatric Pulmonary Medicine Unit, Sihhiye, Ankara, Turkey
Gesierich Wolfgang, Facharzt für Innere Medizin/Pneumologie, Oberarzt der Klinik für Pneumologie, Leiter der Endoskopie, Asklepios-Fachkliniken München Gauting, Germany
Griese Matthias, Kinderklinik und Kinderpoliklinik im Dr. von Haunerschen Kinderspital, University of Munich, Germany
Grutters Jan C., Centre of Interstitial Lung Diseases (cIL), St. Antonius Ziekenhuis Nieuwegein, The Netherlands
Halme Maija, Helsinki University Central Hospital, Division of Respiratory Medicine, Department of Medicine, Helsinki, Finland
Henry Michael, Department of Respiratory Medicine, Cork University Hospital Wilton, Cork, Ireland
Herth Felix JF, Pneumology and Respiratory Care Medicine Thoraxklinik, University of Heidelberg, Germany
Hui-ying Wang, Division of Allergy, Department of Internal Medicine, the Second Affiliated Hospital of Zhejiang University Medical College, Hangzhou, Zhejiang, P. R. China
Ichiwata Toshio, Tokyo Medical University Hachioji Medical Centre, Department of Respiratory Medicine, Tokyo, Japan
Ilkovich Julia M., Pulmonology Department of Pavlov State Medical University, St. Petersburg, Russian Federation
Inoue Yoshikazu, Department of Diffuse Lung Diseases and Respiratory Failure, Clinical Research Centre, National Hospital Organization Kinki-Chuo Chest Medical Centre, Osaka, Japan
Iotti Giorgio A., Department of Anaesthesiology and Intensive Care, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
Irani Sarosh, Pulmonary Department, Kantonsspital Aarau, Switzerland
Kolek Vítězslav, Dept. of Respiratory Medicine, University Hospital, Olomouc, Czech Republic
Morais António, Serviço de Pneumologia- Hospital São João-Porto, Portugal
Morgan Cliff, Department of Critical Care & Anaesthesia, Royal Brompton Hospital, London, UK
Nicolai Thomas, Kinderklinik und Kinderpoliklinik im Dr. von Haunerschen Kinderspital, University of Munich, Germany
Novikova Lubov N., Pulmonology Department of Pavlov State Medical University, St. Petersburg, Russian Federation
Primhak Robert, Sheffield Children’s Hospital, Western Bank, Sheffield, UK
Reiter Karl, Kinderklinik und Kinderpoliklinik im Dr. von Haunerschen Kinderspital, University of Munich, Germany
Retsch-Bogart George, Division of Pulmonology, University of North Carolina at Chapel Hill, NC, USA
Rodi Giuseppe, Department of Anaesthesiology and Intensive Care, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
Russi Eric, Pulmonary Division University Hospital, Zurich, Switzerland
Schmitz Jochen, Lungen Clinic Grosshansdorf, Germany
Schön Carola, Kinderklinik und Kinderpoliklinik im Dr. von Haunerschen Kinderspital, University of Munich, Germany
Schumann Christian, Pulmonology,Thoracic Oncology,Sleep and Respiratory Intensive Care Medicine Kempten-Oberallgäu Hospital Immenstadt, Germany
Veltkamp Marcel, Centre of Interstitial Lung Diseases (cIL), St. Antonius Ziekenhuis Nieuwegein, The Netherlands
Verwey Charl, Division of Paediatric Pulmonology, Department of Paediatrics, Chris Hani Baragwanath Academic Hospital University of the Witwatersrand, Johannesburg, Republic of South Africa
Wood Robert E., Division of Pulmonary Medicine Cincinnati Children’s Hospital, Cincinnati, OH, USA
This study was supported by a grant from the European Union E-Rare JTC 2009: EuPAPnet.
Availability of data and material
The questionnaire used to perform this survey is available in the Additional file 1.
IC, ML, GB an BCT had full access to all of the study data. CT takes responsibility for the integrity of the data and the accuracy of the data analysis. MG, BCT, FB, UC, GR, CHMVM and JG substantially contributed to the study design. IC, ML, GR and BCT drafted and supervised the manuscript. MG, FB, JG, KN, CHMVM, UC, VC, TI, YI, AB, GAI critically revised the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
This survey was born as part of the project entitled EuPAPNet, funded by eRARE Research Programmes on Rare Diseases. We have the approval by our ethics committee regarding the whole EuPAPNet project. The international survey does not collect individual patient data, but averages data from all the participating centres.
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