Complete exon sequencing of all known Usher syndrome genes greatly improves molecular diagnosis

Crystel Bonnet; M'hamed Grati; Sandrine Marlin; Jacqueline Levilliers; Jean-Pierre Hardelin; Marine Parodi; Magali Niasme-Grare; Diana Zelenika; Marc Délépine; Delphine Feldmann; Laurence Jonard; Aziz El-Amraoui; Dominique Weil; Bruno Delobel; Christophe Vincent; Hélène Dollfus; Marie-Madeleine Eliot; Albert David; Catherine Calais; Jacqueline Vigneron; Bettina Montaut-Verient; Dominique Bonneau; Jacques Dubin; Christel Thauvin; Alain Duvillard; Christine Francannet; Thierry Mom; Didier Lacombe; Françoise Duriez; Valérie Drouin-Garraud; Marie-Françoise Thuillier-Obstoy; Sabine Sigaudy; Anne-Marie Frances; Patrick Collignon; Georges Challe; Rémy Couderc; Mark Lathrop; José-Alain Sahel; Jean Weissenbach; Christine Petit; Françoise Denoyelle

doi:10.1186/1750-1172-6-21

Research
Open Access

Complete exon sequencing of all known Usher syndrome genes greatly improves molecular diagnosis

Crystel Bonnet†^{1, 2},
M'hamed Grati†^{1, 2, 30},
Sandrine Marlin¹,
Jacqueline Levilliers²,
Jean-Pierre Hardelin²,
Marine Parodi³,
Magali Niasme-Grare³,
Diana Zelenika⁴,
Marc Délépine⁴,
Delphine Feldmann^{1, 3},
Laurence Jonard^{1, 3},
Aziz El-Amraoui²,
Dominique Weil²,
Bruno Delobel⁵,
Christophe Vincent⁶,
Hélène Dollfus⁷,
Marie-Madeleine Eliot⁸,
Albert David⁹,
Catherine Calais¹⁰,
Jacqueline Vigneron¹¹,
Bettina Montaut-Verient¹²,
Dominique Bonneau¹³,
Jacques Dubin¹⁴,
Christel Thauvin¹⁵,
Alain Duvillard¹⁶,
Christine Francannet¹⁷,
Thierry Mom¹⁸,
Didier Lacombe¹⁹,
Françoise Duriez²⁰,
Valérie Drouin-Garraud²¹,
Marie-Françoise Thuillier-Obstoy²²,
Sabine Sigaudy²³,
Anne-Marie Frances²⁴,
Patrick Collignon²⁴,
Georges Challe²⁵,
Rémy Couderc^{1, 3},
Mark Lathrop⁴,
José-Alain Sahel²⁶,
Jean Weissenbach²⁷,
Christine Petit^{2, 28}Email author and
Françoise Denoyelle^{2, 29}Email author

†Contributed equally

Orphanet Journal of Rare Diseases20116:21

https://doi.org/10.1186/1750-1172-6-21

Received: 4 December 2010

Accepted: 11 May 2011

Published: 11 May 2011

Abstract

Background

Usher syndrome (USH) combines sensorineural deafness with blindness. It is inherited in an autosomal recessive mode. Early diagnosis is critical for adapted educational and patient management choices, and for genetic counseling. To date, nine causative genes have been identified for the three clinical subtypes (USH1, USH2 and USH3). Current diagnostic strategies make use of a genotyping microarray that is based on the previously reported mutations. The purpose of this study was to design a more accurate molecular diagnosis tool.

Methods

We sequenced the 366 coding exons and flanking regions of the nine known USH genes, in 54 USH patients (27 USH1, 21 USH2 and 6 USH3).

Results

Biallelic mutations were detected in 39 patients (72%) and monoallelic mutations in an additional 10 patients (18.5%). In addition to biallelic mutations in one of the USH genes, presumably pathogenic mutations in another USH gene were detected in seven patients (13%), and another patient carried monoallelic mutations in three different USH genes. Notably, none of the USH3 patients carried detectable mutations in the only known USH3 gene, whereas they all carried mutations in USH2 genes. Most importantly, the currently used microarray would have detected only 30 of the 81 different mutations that we found, of which 39 (48%) were novel.

Conclusions

Based on these results, complete exon sequencing of the currently known USH genes stands as a definite improvement for molecular diagnosis of this disease, which is of utmost importance in the perspective of gene therapy.

Keywords

Missense Mutation
Retinitis Pigmentosa
Pathogenic Mutation
Splice Site Mutation
Usher Syndrome

Background

Usher syndrome (USH, MIM 276900, MIM 276905, MIM 605472) combines sensorineural hearing impairment with retinitis pigmentosa [1]. In addition, vestibular dysfunction can be observed in some patients. USH occurs in ~1/20 000 individuals, and represents 50% of all monogenic deaf-blindness cases. Three clinical subtypes can be distinguished. USH type I (USH1) is characterized by severe to profound congenital hearing impairment, prepubertal onset of retinitis pigmentosa, and vestibular arreflexia. USH type II (USH2) combines congenital moderate to severe hearing impairment, onset of retinitis pigmentosa in the first or second decade of life, and absence of vestibular dysfunction. Finally, USH type III (USH3) patients present with congenital or early onset progressive hearing impairment, variable age of onset and severity of retinitis pigmentosa, and variable vestibular dysfunction. USH is inherited in the autosomal recessive mode, and is genetically heterogeneous. To date, nine causative genes have been identified. Mutations in MYO7A[2], USH1C[3, 4], CDH23[5, 6], PCDH15[7, 8] and USH1G[9] cause USH1, mutations in USH2A[10], VLGR1[11] and WHRN[12] cause USH2, and mutations in USH3A[13] cause USH3. Mutations in MYO7A[14–16], USH1C[17, 18], CDH23[6], PCDH15[17] and WHRN[19] have also been reported in patients affected by hearing impairment only, while USH2A is also involved in isolated retinitis pigmentosa [20].

The USH1 genes encode the actin-based motor protein myosin VIIa (USH1B), two Ca²⁺-dependent transmembrane adhesion proteins, cadherin-23 (USH1D) and protocadherin-15 (USH1F), the PDZ domain-containing submembrane protein harmonin (USH1C), and the scaffold protein sans that contains ankyrin repeats and a sterile alpha motif domain (USH1G). The USH2 genes encode two large transmembrane proteins, usherin (USH2A) and VLGR1 (very large G protein-coupled receptor, USH2C), and the PDZ domain-containing submembrane protein whirlin (USH2D). Finally, USH3A encodes the four-transmembrane-domain protein clarin-1. Each USH gene encodes several protein isoforms, except MYO7A and USH1G.

Absence of an early diagnosis of USH is devastating. In USH1 patients, sign language becomes a less and less efficient mode of communication as the visual defect progresses, and ultimately, the patients may become unable to communicate except by tactile exchanges. As a result of an early diagnosis of USH1, early bilateral cochlear implantation allowing the development of an oral mode of communication and early physical therapy for vestibular disorders are strongly recommended. The early diagnosis is also critical for genetic counseling, educational orientation and therapeutic management, which may include retinal gene therapy in the future [21, 22]. So far, a comprehensive molecular diagnosis of USH has been hampered both by the genetic heterogeneity of the disease and the large number of exons for six out of the nine known USH genes. The five USH1, three USH2, and one USH3 genes are collectively composed of 183, 173, and five coding exons, respectively [23].

Cremers and collaborators have developed a genotyping microarray for USH, based on the arrayed primer extension (APEX) method. This approach, in a first version, included the analysis of 298 USH-associated sequence variants located in eight genes: MYO7A, USH1C, CDH23, PCDH15, USH1G, USH2A, VLGR1 and USH3A[24]. The mutations detected by the array subsequently increased, and currently include 612 previously identified disease-associated variants in the nine known USH genes [25]. The selected variants were prevalent in the following European countries: Belgium, Denmark, UK, Germany, Italy, Spain, Switzerland and Netherlands, and in the USA. The authors could prove that the chip, with >98% accuracy, is an adaptable and affordable mutation screening tool. However, the efficiency of the chip was both dependent on the USH subtype examined and the studied population, ranging from 30% in the USA to 80% in Denmark in USH1 cases [24]. Recently, Jaijo et al., using an intermediate genotyping microarray (429 reported mutations), found mutations in only 34% of the patients tested [26], which is indicative of a large number of private mutations. Therefore, improvement of the molecular diagnosis is needed.

Alternative strategies include direct sequencing of USH gene coding exons [27–30]. To determine the most efficient strategy, some critical information is, however, still lacking. Is the clinically diagnosed USH subtype a reliable indication of the causative gene? What is the frequency of digenic/oligogenic inheritance in this disease? Such a mode of inheritance is suggested by the colocalization and direct in vitro interactions of the USH1 proteins [31–39], and of the USH2 proteins [40, 41]. In a few USH1 patients, digenic inheritance involving PCDH15 and CDH23 has indeed been reported [42]. To address these issues, we undertook a large-scale mutation screening of all currently known USH genes in a cohort of 54 USH patients.

Subjects and Methods

Subjects

Fifty-four unrelated Caucasian patients including five patients originating from Maghreb were included in the study. Most patients were referred to Armand-Trousseau Children's Hospital in Paris, and other patients were referred to genetic consultations throughout France. All patients were tested by audiograms and electroretinogram. Auditory function was assessed by otoscopy, tympanometry, standard pure tone audiometry, and recording of auditory brainstem responses and otoacoustic emissions. The cochlear origin of the hearing impairment was confirmed by auditory brainstem responses, and by the absence of otoacoustic emissions. USH was diagnosed on the basis of simultaneous occurrence of sensorineural deafness and retinal degeneration. Scrutiny of the time of onset, evolution and severity of the hearing impairment, and quality of vestibular responses enabled to assign the patients to one of the three clinical types of the disease [43]. Patients were considered as USH3 when their hearing impairment had been detected in adulthood and showed clear progressiveness. For these patients, vestibular function determined by caloric tests was normal. Parents of most of the patients were available for the study, and had normal hearing. This study was approved by the local ethics committee, and written consent for genetic testing was obtained from adult probands or parents of minor patients.

PCR amplification and sequencing

Genomic DNA was extracted from peripheral blood using standard procedures. The coding exons and flanking intronic sequences of all nine USH genes were amplified and sequenced using forward and reverse primers (primer sequences and conditions available upon request). We also searched for the previously reported 684 kb deletion in PCDH15 using the reported primers [44]. Sequences were run on ABI 3100 DNA analyzer, and assembled using ABI Prism Seqscape 2.1 to Genbank reference sequences [45].

Control DNAs

The genomic DNAs from 153 unaffected Caucasian control individuals were sequenced (306 control alleles). For the mutations possibly involved in oligogenic inheritance, DNAs from 333 healthy unrelated Caucasian individuals were used as controls. For the mutations present in patients originating from Maghreb, the DNAs from 95 Moroccan and 91 Algerian healthy unrelated individuals were used as controls.

In silico analysis of sequence variants

The SIFT (Sorting Intolerant from Tolerant) [46] and Polyphen [47] software programs were used to predict the influence of any amino acid substitution on the protein structure and function. NetGene2 [48] and "Splice site prediction by neural network" [49] interfaces were used to predict the influence of nucleic acid substitutions on splice donor and acceptor sites. Presence of Exonic Splicing Enhancers (ESE) was detected using ESE Finder [50].

Segregation analysis

Segregation of all sequence variants identified in the patients was studied by sequencing the corresponding DNA fragments in the parents and other relatives. In all patients carrying two distinct mutations in a given USH gene, biallelic transmission was confirmed by the segregation analysis.

Mutation nomenclature

The mutation nomenclature complies with the mutation nomenclature correction tool Mutalyzer [51] according to the HGVS Guidelines & Recommendations [52]. The +1 position in mutation numbering corresponds to the A of the ATG initiation codon.

Protein Accession numbers

MYO7A, [Swiss-Prot:Q13402]; USH1C, [Swiss-Prot:Q7RTU8]; CDH23, [Swiss-Prot:Q9H251]; PCDH15-CD1, [Swiss-Prot:Q96QU1]; PCDH15-CD2, [NCBI-RefSeq:NP_001136241.1]; PCDH15-CD3, [Swiss-Prot:C9J4F3]; USH1G, [Swiss-Prot:Q495M9]; USH2A, [Swiss-Prot:075445]; VLGR1, [Swiss-Prot:Q8WXG9]; WHRN, [Swiss-Prot:Q9P202]; USH3A, [Swiss-Prot:P58418] and [Swiss-Prot:P58418-1] for "a" and "c" variants, respectively.

Results

Mutation analysis: high prevalence of novel mutations

We analyzed the nine USH genes in a cohort of 54 French patients, of whom 27 were affected by USH1, 21 by USH2, and six by USH3. From the patient and parent questionnaires, consanguinity was established for nine families (see Table 1). Sequencing of the coding and non coding exons of all currently known USH genes was carried out in every patient. Screening for predicted causative missense and splice site mutations was performed using prediction software programs. Amino acid substitutions were considered likely to be pathogenic missense mutations when predicted possibly or probably deleterious by Polyphen software and not-tolerated by the SIFT program. Nucleotide variations were considered likely to be splice site mutations when predicted highly confident donor or acceptor site mutations by Netgene2 and "Splice site prediction by neural network" programs. These sequence variants were ultimately classified as presumably pathogenic mutations only if the affected amino acid residues were evolutionarily conserved (Additional file 1 Figures S1 to S3) and/or these variants were not found in the control individuals (see Subjects and Methods).

Table 1

Genotypes of USH patients

Genes			MYO7A	USH1C	CDH23	PCDH15	USH1G	USH2A	VLGR1	WHRN
Patient		USH type
U37		I	[p.R666X] + [p.E1917X]
U57		I	[p.C1198X]+ [p.R1240Q]
P0485		I	[p.Q1798X] + [p.E1917X]
U14	C	I	[p.R972X] + [p.R972X]
U9	C	I	[p.K164X] + [p.K164X]
U36		I	[p.R2024X] + [p.G519D]		[p.R1060W]
U20		I	[p.R669X] + [p.R1883Q]
P0505		I	[p.Q1798X] + [p.A2009fsX32]
S1556	C	I	[p.H133fsX7] + [p.H133fsX7]
S1295	C	I	[p.Y1302fsX97] + [p.Y1302sX97]					[p.G1301V]	[p.Q5459H]
P0504		I	[p.D75fsX31] + [p.R1240Q]	[p.R357W]
U45		I	[p.D75fsX31] + [p.T165M]
P0411	C	I	[c.2283-1G>T] + [c.2283-1G>T]						[p.D4707Y]
P0070		I	[p.G163R] + [p.A198T]
P0052		I	[c.1690+1G>A] + [p.F1963del]
U3		I	[p.L2186P]				[p.L16V]	[p.C3307W]
DID	C	I		[p.R80fsX69] + [p.R80fsX69]	[p.R3043W]
U47		I		[p.R80fsX69] + [p.R103H]
P0469		I			[p.E2135fsX3] + [c.6050-9G>A]
S1212		I			[p.R1379P] + [p.D2639G]
U38		I				[p.R991X] + [p.R991X]
S1530		I				[p.R1273S]
P0257		I					[p.W38X]
S1273		I					[p.D29fsX29] + [p.D29fsX29]
U46		I
U50		I
S1823	C	I
P0486		II	[p.A457V] + [p.K269del]
U6		II						[p.E3562X] + [p.E767fsX21]
U24		II	[p.P1220L]					[p.S1307X] + [p.C536R]
U48		II						[p.W3955X] + [p.R2509fsX19]
P0483		II						[p.E1492X] + [p.T3571M]
P0418		II	[p.K268R]					[p.S5030X]
U56	C	II						[p.T2991fsX61] + [p.T2991fsX61]
U42		II						[p.E767fsX21] + [p.Y4128fsX24]
P0449		II						[p.E767fsX21] + [p.C575Y]
P0493		II						[p.H308fsX16] + [p.T4809I]
P0432		II			[p.R1189W]			[p.M1344fsX42]
U51		II						[p.V218E] + [p.R317R]
P0511		II						[p.T3571M] + [p.T352I]
U49		II							[p.E4321X] + [p.Q753fsX8]
P0473		II							[p.P522fsX8] + [p.M5890fsX10]	[p.S11R]
U58		II							[p.F112fsX29] + [p.H3399P]
P0463		II							[p.E4186fsX17]
U10		II
U53		II								[p.P246fsX13] + [p.P246fsX13]
U19	C	II			[p.H755Y]
P0426		II
U21		III						[p.Y1730fsX6] + [c.10586-1G>C]
U30		III						[p.E767fsX21] + [p.R303H]
S1226		III						[p.G2752R] + [c.5776+1G>A]
P0239		III							[p.N4885S]
P0484		III							[p.D1944N]
P0069		III								[p.R379W]

Novel mutations are in bold. C (2^nd column) denotes consanguinity.

A total of 81 distinct, presumably pathogenic mutations were detected, specifically, 16 nonsense mutations, five nucleotide duplications, 17 frame-shifting deletions, seven splicing defect-causing mutations, 34 missense mutations, and one isocoding variation. Thirty-nine (48%) of these mutations, i.e. 27% to 100% of the mutations found in each USH gene, had not been previously reported (Tables 2, 3 and 4, Figure 1). In addition, 103 amino acid substitutions were classified as presumably nonpathogenic sequence variants, including 33 new variants and six variants that had previously been reported as pathogenic mutations (Table 5). Numerous, presumably neutral, isocoding and intronic variants were also observed (listed in Additional file 2, Table S1).

Table 2

Pathogenic DNA variants

Gene	Nucleotide change	Exon	Amino acid change	Frequency in USH alleles (×/108)	Frequency in control alleles	Patient origin & reference
MYO7A
	223delG	4	D75fsX31	2		Australia, Italy, France [78]
	397dupC	5	H133fsX7	2		This study
	490A>T	6	K164X	2		This study
	592G>A	6	A198T + splice defect	1	0/306	Algeria [27]
	1556G>A	14	G519D/splice defect	1	0/306	USA, France [63]
	1690+1G>A	14	Splice defect	1		This study
	1996C>T	17	R666X	1		Great Britain, Denmark [62]
	2005C>T	17	R669X	1		USA [24]
	2283-1G>T	20	Splice defect	2		Algeria [27]
	2914C>T	24	R972X	2		Pakistan [79]
	3594C>A	28	C1198X	1		This study
	3904delT	30	Y1302fsX97	2		This study
	5392C>T	39	Q1798X	2		Denmark, German, Great Britain/France [62]
	5749G>T	42	E1917X	2		unknown [80]
	6025delG	44	A2009fsX32	1		Spain [63]
	6070C>T	45	R2024X	1		unknown [80]
USH1C
	238_239dupC	3	R80fsX69	3		Pakistan, Europe, Guinea [4]
CDH23
	6050-9G>A	46	Splice defect	1		Germany [54]
	6404_6405delAG	47	E2135fsX31	1		This study
PCDH15
	2971C>T	22	R991X	2		France [27]
USH1G
	84dupC	1	D29fsX29	2		This study
	113G>A	1	W38X	1		USA [58]
USH2A
	920_923dupGCCA	6	H308fsX16	1		Denmark [81]
	2299delG	13	E767fsX21	4		Europe, USA, Africa, China [10]
	3920C>G	18	S1307X	1		France [82]
	4030_4037delATGGCTGG	18	M1344fsX42	1		This study
	4474G>T	21	E1492X	1		Spain [83]
	5189_5199delATATGTTTCAT	26	Y1730fsX6	1		This study
	5776+1G>A	28	Splice defect	1		Norway [28]
	7522delT	40	R2509fsX19	1		This study
	8970_8971delCA	45	T2991fsX61	2		This study
	10586-1G>C	54	Splice defect	1		This study
	10684G>T	54	E3562X	1		Denmark, Norway [28]
	11864G>A	61	W3955X	1		Netherlands [84]
	12381_12382delCT	63	Y4128fsX24	1		This study
	15089C>A	70	S5030X	1		France [66]
VLGR1
	333_334delTT	3	F112fsX29	1		This study
	1563dupT	9	P522fsX8	1		This study
	2258_2270delAAGTGCTGAAATC	12	Q753fsX8	1		This study
	12552_12553delGG	62	E4186fsX17	1		This study
	12961G>T	64	E4321X	1		This study
	17668_17669delAT	82	M5890fsX10	1		This study
WHRN
	737delC	2	P246fsX13	2		This study

Novel mutations are in bold.

Table 3

Presumably pathogenic DNA variants

Gene	Nucleotide change	Exon	Amino acid change	Protein domain	Frequency in USH alleles (×/108)	Frequency in control alleles	Patient origin & reference
MYO7A
	487G>C	6	G163R	Motor head	1	0/306	Algeria [27]
	494C>T	6	T165M	Motor head	1	0/306	Great Britain, France [58]
	803A>G	8	K268R	Motor head	1	0/306	This study
	805_807delAAG	8	K269del	Motor head	1	0/306	Italy, France [63]
	1370C>T	13	A457V	Motor head	1	0/306	Ireland, France [63]
	3659C>T	29	P1220L	MyTH4 (1)	1	0/666	This study
	3719G>A	29	R1240Q	MyTH4 (1)	2	0/306	Denmark, Great Britain/France [62]
	5648G>A	41	R1883Q	MyTH4 (2)	1	0/306	USA [58]
	5887_5889delTTC	43	F1963del	FERM (2)	1		Europe, USA [24]
	6657T>C	48	L2186P	FERM (2)	1	0/666	France [85]
USH1C
	308G>A	4	R103H	PDZ1	1	0/306	France [27]
	1069C>T	13	R357W	Coiled-coil	1	0/498	This study
CDH23
	2263C>T	20	H755Y	cd7	1	0/306	USA [56]
	3178C>T	26	R1060W	cd10	1	0/626	Europe [55]
	3565C>T	29	R1189W	cd11	1	0/306	This study
	4136G>C	33	R1379P	cd13	1	0/306	This study
	7916A>G	55	D2639G	cd25	1	0/306	This study
	9127C>T	62	R3043W	adjacent to TM (extracellular)	1	0/490	This study
PCDH15
	3817C>A	29	R1273S	cd11	1	0/306	This study
USH1G
	46C>G	1	L16V		1	0/666	This study
USH2A
	653T>A	4	V218E	Nter laminin	1	0/306	Great Britain [86]
	908G>A	6	R303H	Nter laminin	1	0/306	USA [87]
	949C>A	6	R317R	Nter laminin	1	0/306	Netherlands [60]
	1055C>T	6	T352I	Nter laminin	1	0/306	Norway [28]
	1606T>C	9	C536R	1^st laminin EGF-like	1	0/306	Denmark [81]
	1724G>A	10	C575Y	2^nd laminin EGF-like	1	0/306	This study
	3902G>T	18	G1301V	14^th FnIII	1	0/484	This study
	8254G>A	42	G2752R	3^rd laminin EGF-like	1	0/306	Japan [88]
	9921T>G	50	C3307W	18^th-19^th FnIII	1	0/482	This study
	10712C>T	54	T3571M	20^th FnIII	2	0/306	Spain [89]
	14426C>T	66	T4809I	33^rd FnIII	1	0/306	Canada [90]
VLGR1
	5830G>A	28	D1944N	13^th -14^th β-Calx	1	0/306	This study
	10196A>C	49	H3399P	4^th EAR	1	0/306	This study
	14119G>T	70	D4707Y	32^nd β-Calx	1	0/446	This study
	14654A>G	71	N4885S	32^nd -33^rd β-Calx	1	0/486	This study
	16377G>T	77	Q5459H	35^th β-Calx	1	0/402	This study
WHRN
	33C>G	1	S11R	A/G/S rich region	1	0/494	This study
	1135C>T	4	R379W	PDZ2	1	0/306	This study

Novel mutations are in bold.

Table 4

Distribution of the pathogenic and presumably pathogenic mutations

	Pathogenic and presumably pathogenic mutations (Novel mutations)
MYO7A	26 (7)
USH1C	3 (1)
CDH23	8 (5)
PCDH15	2 (1)
USH1G	3 (2)
USH2A	25 (9)
VLGR1	11 (11)
WHRN	3 (3)
USH3A	0

Figure 1
**Schematic representation of USH1 and USH2 proteins and localization of the novel, presumably pathogenic mutations**. The long isoform of each USH protein is shown. *Splice site mutations. Abbreviations: *IQ motifs*, isoleucine-glutamine motifs; *SAH*, stable single α-helix; *MyTH4*, myosin tail homology 4; *FERM*, band 4.1-ezrin-radixin-moesin; *PDZ*, PSD95, discs large, ZO-1; *PST*, proline-serine-threonine-rich region; EC, extracellular cadherin; TM, transmembrane domain; *Ank*, ankyrin domains; *cent*, central region; *SAM*, sterile alpha motif; *LamG*, laminin G; *LamG/TspN/PTX*, N-terminal thrombospondin/pentaxin/laminin G-like domain; *LamNT*, laminin N-terminal; *EGF* *Lam*, laminin-type EGF-like; *FnIII*, fibronectin type III; *VLGR1*, very large G protein-coupled receptor 1; *Calx*, Ca²⁺-binding calcium exchanger β; *EAR*, Epilepsy Associated Repeats; *Ala/Gly/Ser rich*, alanine, glycine, and serine rich region; *Pro rich*, proline rich region.

Table 5

Presumably neutral missense variants

Gene	Nucleotide change	Exon	Amino acid change	Frequency in USH alleles (×/108)	Frequency in control alleles	References
MYO7A
	47T>C	3	L16S	>10		[58]
	905G>A	9	R302H	2	1/494	[78]
	4996A>T	36	S1666C	>10		U39226
	5156A>G	37	Y1719C*	3	2/306	[91]
	5860C>A	43	L1954I	>10		U39226
USH1C
	2192G>A	21	R731Q	1		This study
	2457G>C	24	E819D	>10		[92]
CDH23
	7C>T	1	R3C	>10		[55]
	1469G>C	14	G490A	>10		[55]
	1487G>A	14	S496N	>10		[55]
	3625A>G	30	T1209A*	1	5/486	[55]
	3664G>A	30	A1222T	4		[55]
	4051G>A	31	D1351N	>10		[55]
	4310G>A	34	R1437Q	6		[55]
	4723A>G	37	T1575A	>10		[55]
	4858G>A	38	V1620M	1	2/306	[55]
	5023G>A	38	V1675I	>10		[55]
	5411G>A	41	R1804Q	>10		[55]
	5418C>G	41	D1806E	2		[93]
	5692G>A	42	A1898T	1	0/306	This study
	5996C>G	45	T1999S	>10		[55]
	6130G>A	46	E2044K	>10		[55]
	6197G>A	46	R2066Q	1	0/306	[55]
	6329C>T	47	A2110V	1		This study
	6596T>A	47	I2199N	1	0/306	This study
	6809G>A	48	R2270H	1		This study
	6847G>A	49	V2283I	6		[55]
	6869C>T	49	T2290M	1	0/306	This study
	7073G>A	50	R2358Q	>10		[55]
	7139C>T	50	P2380L	>10		[55]
	7762G>C	54	E2588Q	1	1/306	[55]
	9049G>A	61	D3017N	1		This study
	9373T>C	65	F3125L	1	7/306	[56]
	9949G>A	69	A3317T	1	1/306	This study
PCDH15
	55T>G	2	S19A	>10		[94]
	1039C>T	10	L347F	1	3/666	This study
	1138G>A	11	G380S	>10		This study
	1304A>C	11	D435A	>10		AL834134
	1910A>G	15	N637S	2		[92]
	2786G>A	21	R929Q	>10		AL834134
	4850A>G	34^§	N1617S	2		This study
	4853A>C	36^§	E1618A	>10		This study
	4982A>C	37^§	Q1661P	>10		This study
USH2A
	373G>A	2	A125T	>10		[95]
	1434G>C	8	E478D	3		[95]
	1663C>G	10	L555V*	1	0/306	[96]
	1931A>T	11	D644V	>10		[95]
	4457G>A	21	R1486K	>10		AF055580
	4994T>C	25	I1665T	>10		[89]
	6317T>C	32	I2106T	>10		[89]
	6506T>C	34	I2169T	>10		[89]
	6713A>C	35	E2238A	6	5/306	[89]
	6875G>A	36	R2292H	4		[28]
	8624G>A	43	R2875Q	4		[89]
	8656C>T	43	L2886F	4		[89]
	9008T>C	45	V3003A	1		This study
	9262G>A	47	E3088K*	1	3/306	[28]
	9296A>G	47	N3099S	4		[89]
	9343A>G	47	T3115A	3	5/306	[28]
	9430G>A	48	D3144N	4		[89]
	9595A>G	49	N3199D	6		[28]
	10232A>C	52	E3411A	>10		[89]
	11504C>T	59	T3835I	>10		[28]
	11602A>G	60	M3868V	>10		[89]
	11677C>A	60	P3893T*	1	1/306	[28]
	15091C>T	70	R5031W	2	2/306	[28]
	15377T>C	71	I5126T*	3	2/306	[87]
VLGR1
	365C>T	4	S122L	>10		This study
	P194H	6	P194H	1	5/468	This study
	1033C>A	7	Q345K	1		This study
	2261T>C	12	V754A	1	0/306	This study
	3289G>A	17	G1097S	1	3/478	This study
	3482C>G	19	S1161C	1	0/306	This study
	4939A>G	23	I1647V	>10		This study
	5780C>T	28	T1927M	>10		[11]
	5851G>A	28	V1951I	>10		[11]
	5953A>G	28	N1985D	>10		[11]
	5960C>T	28	P1987L	>10		[11]
	6012G>T	28	L2004F	>10		[11]
	6695A>G	30	Y2232C	>10		[11]
	7034A>G	32	N2345S	>10		[11]
	7582C>T	33	P2528S	1	1/306	This study
	7751A>G	33	N2584S	>10		[97]
	8291C>T	36	S2764L	6		[11]
	8407G>A	37	A2803T	4		[11]
	9280G>A	43	V3094I	>10		This study
	9743G>A	45	G3248D	>10		[11]
	9650C>T	45	A3217V	2		[11]
	10411G>A	49	E3471K	>10		[97]
	10429G>T	50	D3477Y	1		This study
	10490A>G	50	Q3497R	1		This study
	10577T>C	51	M3526T	3		This study
	10936T>C	52	S3646P	3		This study
	11599G>A	56	E3867K	>10		This study
	12269C>A	59	T4090N	2		This study
	14029T>C	69	F4677L	1	2/478	This study
	14905T>C	73	W4969R	2		This study
	17626G>A	82	V5876I	>10		This study
	18475A>G	88	M6159V	2		This study
WHRN
	229A>T	1	T77S	1	1/468	[98]
	979C>A	4	L327I	1		This study
	1318G>A	6	A440T	>10		[99]
	1838T>C	9	M613T	>10		This study
	2348T>C	10	V783A	>10		[99]
	2388C>A	10	N796K	>10		[99]

Novel mutations are in bold. * Mutations considered pathogenic by prediction Software, but excluded by segregation studies. ^§ Exons 34, 36 and 37 are specific to isoforms CD1, CD2 and CD3, respectively.

The pathogenicity of several exonic variants found in our patients and predicted to be pathogenic in previous studies and/or by prediction software was further investigated. The p.T1209A missense mutation in CDH23 has previously been reported in two affected families and considered as pathogenic [55, 58]. However, we found it in five of 486 control alleles from French and Maghreban populations. The p.Y1719C missense mutation in MYO7A seems to represent a frequent sequence variant in the Moroccan population, with an estimated carrier frequency of 0.07 [100], and was observed in three out of 306 control alleles. The p.R302H mutation in MYO7A, which affects a residue within a non-conserved region of the motor domain, was detected in one out of 494 control alleles. Moreover, two of five different MYO7A cDNA clones isolated from three independent libraries were found to encode a histidine residue at codon position 302 [101], which further argues in favor of a non-pathogenic sequence variant. The p.E3088K missense mutation in USH2A, previously described by Dreyer et al., was present in three out of 306 control alleles, which argues in favor of a non-pathogenic sequence variant [26, 28]. The missense mutation p.I5126T in USH2A has been reported as likely pathogenic [87]. We found it in two USH1 patients, who in addition carried two pathogenic mutations in MYO7A. We detected it in two individuals from the French control population, suggesting that it is a non-pathogenic sequence variant. The p.L555V mutation in USH2A has been found in homozygous state in one Spanish patient, together with a biallelic splice site variant (c.1841-2A>G) [26]. Numerous, presumably neutral, isocoding and intronic variants were also observed (listed in Additional file 2Table S1).

Twenty-six pathogenic or presumably pathogenic mutations in MYO7A were found in 19 patients, specifically, eight nonsense mutations, one nucleotide duplication, five nucleotide deletions, four splice site mutations, and eight missense mutations. Seven of these mutations had not been previously reported, including two nonsense mutations (p.K164X, p.C1198X), a nucleotide duplication (c.397dupC; p.H133fsX7), a nucleotide deletion c.3904delT (p.Y1302fsX97), a nucleotide substitution (c.1690+1G>A) predicted to alter the splice donor site of intron 14, and two missense mutations (p.K268R and p.P1220L) that change amino acid residues located in the motor head and the first MyTH4 domain of the myosin VIIa tail, respectively (Tables 2, 3 and Figure 1).

Three distinct pathogenic or presumably pathogenic mutations in USH1C were detected in three patients, specifically, a nucleotide duplication (c.238_239dupC; p.R80fsX69) already reported in several patients [3, 4, 27, 53], a known missense mutation (p.R103H) affecting an amino acid residue located in the PDZ1 domain of the protein [27], and a novel missense mutation (p.R357W), predicted to affect the first coiled-coil domain of the protein. These mutations are expected to affect the three classes of harmonin isoforms (Tables 2, 3, Figure 1) [4].

Eight pathogenic or presumably pathogenic mutations in CDH23 were found in six patients, specifically, a previously reported mutation that affects splicing (c.6050-9G>A) [54], a novel nucleotide deletion (c.6404_6405delAG; p.E2135fsX31), and six missense mutations [55, 56], four of which (p.R1189W, p.R1379P, p.D2639G, and p.R3043W) had not been previously reported. They affect amino acid residues located in the 11^th, 13^th and 25^th cadherin repeat and the extracellular region adjacent to the transmembrane domain (3065-3085), respectively (Tables 2, 3 Figure 1). Intriguingly, the p.R1060W mutation, which affects a residue in the 10^th cadherin repeat that belongs to a canonical motif (DRE) predicted to bind Ca²⁺[57], has previously been reported in an isolated form of deafness, DFNB12 (cited in Astuto et al. [55]).

Two pathogenic or presumably pathogenic mutations in PCDH15, specifically, a nonsense mutation (p.R991X) [27] and a novel missense mutation (p.R1273S), were found in two patients. The missense mutation affects an amino acid residue located immediately after the 11^th cadherin repeat (Tables 2, 3, Figure 1). The large genomic rearrangement in PCDH15 previously reported by Le Guedard et al. [44] was not detected in this group of patients.

Three pathogenic or presumably pathogenic mutations in USH1G were found in three patients, specifically, an already reported nonsense mutation (p.W38X) [58], a novel nucleotide duplication (c.84dupC; p.D29fsX29), and a novel sequence variant (c.46C>G; p.L16V). This variant was absent from the control DNAs (0/666 alleles) and, according to the prediction software programs (NetGene2 and ESE finder), should create a splice donor site resulting in a premature stop codon at codon position 17 (Tables 2, 3; Figure 1).

Twenty-five pathogenic or presumably pathogenic mutations in USH2A were found in 17 patients including three USH3 patients, specifically, five nonsense mutations, one nucleotide duplication, six nucleotide deletions [59], two splice site mutations, 10 missense mutations, and one isocoding variation possibly creating a splice donor site (Tables 2, 3). All these mutations affect the extracellular region of usherin (Figure 1). Nine mutations had not been previously reported, specifically, five frame-shifting deletions (c.4030_4037delATGGCTGG/p.M1344fsX42, c.5189_5199delATATGTTTCAT/p.Y1730fsX6, c.7522delT/p.R2509fsX19, c.8970_8971delCA/p.T2991fsX61, and c.12381_12382delCT/p.Y4128fsX24), one splice acceptor site mutation (c.10586-1G>C) that is expected to result in exon 54 skipping and premature termination of the protein, and three missense mutations (p.C575Y, p.G1301V, p.C3307W) that affect amino acid residues located in the 14^th fibronectin type III domain and the trideca-di-cysteine domain (residue 3192 to 3371) between the 18^th and the 19^th fibronectin type III domains (Figure 1). Notably, the isocoding mutation (c.949C>A; p.R317R) has been predicted to be pathogenic by Pennings [60] and considered as nonpathogenic by Dreyer [28]. Segregation analysis in our family was compatible with a pathogenic effect of this mutation (Additional file 1 Figure S4).

Eleven pathogenic or presumably pathogenic mutations in VLGR1 were detected in eight patients including two USH3 patients. All were novel mutations, specifically, a nonsense mutation (p.E4321X), a nucleotide duplication (c.1563dupT; p.P552fsX8), four nucleotide deletions (c.333_334delTT/p.F112fsX29, c.2258_2270delAAGTGCTGAAATC/p.Q753fsX8, c.12552_12553delGG/p.E4186fsX17), and c.17668_17669delAT/p.M5890fsX10), and five missense mutations (p.D1944N, p.H3399P, p.D4707Y, p.N4885S, p.Q5459H) that all affect amino acid residues located in the large extracellular region of the protein, between the 13^th and 14^th β-Calx domains, in the 4^th Epilepsy Associated Repeat domain, in the 32^nd β-Calx domain, between the 32^nd and 33^rd β-Calx domains, and in the 35^th β-Calx domain, respectively (Tables 2, 3, Figure 1).

Three pathogenic or presumably pathogenic mutations in WHRN were detected in three patients including one USH3 patient, specifically, a novel deletion (c.737delC; p.P246fsX13), and two novel missense mutations (p.S11R and p.R379W) that affect amino acid residues located in the N-terminal Ala/Gly/Ser-rich stretch (aa 9-31) and immediately after the PDZ2 domain, respectively (Tables 2, 3, Figure 1). Notably, these missense mutations only affect the longer whirlin isoform [19], which is a component of the ankle link molecular complex together with VLGR1 and usherin [40, 41].

No mutations in USH3A were detected in our series of USH patients.

Transmission modes: evidence for digenic/oligogenic inheritance in some patients

We found mutations in 49 out of 54 (91%) USH patients, specifically, in 24 out of 27 (89%) USH1 patients, 19 out of 21 (90%) USH2 patients, and all six (100%) USH3 patients (see Table 1). Mutations in MYO7A, USH1C, CDH23, PCDH15, and USH1G, were found in 55%, 7%, 7%, 7%, and 4% of the USH1 cases, respectively. Mutations were detected on both alleles in 21 USH1 patients (including the six consanguineous families), and on one allele in the remaining three USH1 patients. Moreover, one of these patients (U3) harboured monoallelic, presumably pathogenic mutations in two different USH1 genes (see below). Mutations in USH2A, VLGR1 and WHRN were found in 57%, 19% and 9.5% of the USH2 cases, respectively. Notably, one USH2 patient (P0486) carried biallelic mutations in MYO7A. Mutations were detected on both alleles in 15 USH2 patients (including a consanguineous family), and on one allele in the remaining four USH2 patients. Finally, as regards the USH3 patients, biallelic mutations in USH2A and monoallelic mutations in VLGR1 or WHRN were found in three patients, two patients, and one patient, respectively.

One USH1 and two USH2 patients were heterozygotes for mutations in two or three USH genes, suggesting a possible digenic/oligogenic inheritance of the syndrome. In the USH2 patients, however, segregation analysis did not support digenic inheritance. Patient P0418 carries a nonsense mutation in USH2A (p.S5030X) and a missense mutation in MYO7A (p.K268R), but his brother, who is also clinically affected, does not carry the MYO7A mutation. Patient P0432 has a c.4030_4037delATGGCTGG (p.M1344fsX42) mutation in USH2A and a missense mutation in CDH23 (p.R1189W), but his father, who has neither deafness nor retinitis pigmentosa, also carries these two mutations, and his clinically affected sister does not carry the mutation in CDH23. In the USH1 patient, we found three presumably pathogenic mutations in MYO7A (c.6657T>C), USH1G (c.46C>G; p.L16V) and USH2A (c.9921T>G). Her father carries the mutations in MYO7A and USH2A without displaying symptoms of the disease, whilst her unaffected mother carries the mutation in USH1G. The mutations in MYO7A, USH1G and USH2A were not found in 666 control alleles. Of the four siblings, the affected girl is the only one who carries the mutations in MYO7A and USH1G, and, all the more, the mutations in the three genes (Figure 2). Therefore, a combination of monoallelic mutations in three USH genes may be responsible for the disease in this patient.

Figure 2
**Segregation of the mutations in** MYO7A , USH1G **and** USH2A **in family U3**. Arrow indicates the deaf proband.

Seven patients out of 54 (13%) carried two presumably pathogenic mutations in an USH gene, plus one or two additional mutations in another USH gene. Taking into account only the 39 patients for whom biallelic mutations have been identified, 18% (7 out of 39) carry additional mutations. Specifically, five USH1 patients carried biallelic mutations in an USH1 gene plus one or two additional mutations in another USH1 (three patients) or USH2 (two patients) gene, and two USH2 patients carried biallelic mutations in USH2 genes plus one additional, presumably pathogenic mutation in an USH1 or an USH2 gene (Table 1). Parents and siblings available in six out of seven families indeed showed that the two mutations present in the same gene originated from one parent each (Figure 3). The mutations found in the genes that were mutated on both alleles in the patients consist of two nonsense mutations, five nucleotide deletions, one splice site mutation, and three missense mutations. The eight additional mutations found in these patients were amino acid substitutions that were predicted "probably damaging" and "not tolerated" by Polyphen and SIFT program, respectively. One of these mutations, p.R1060W in CDH23, has already been reported in USH patients [55].

Figure 3
**Genetic evidence for presumably pathogenic mutations in more than one USH gene in six families**. The index case in family U24 is indicated by an arrow.

Discussion

The major goal of the study was to design a powerful and reliable strategy for molecular diagnosis of USH. For that purpose, some essential, so far missing information was gathered by: i) comparing the strategy for mutation detection currently in use with the here developed USH exome sequencing (including splice sites), ii) determination of whether the phenotype can restrict the mutation screening to the USH genes corresponding to the clinical subtype in a given patient, and iii) defining the possible existence of digenic/oligogenic inheritance of the disease in some patients.

We found mutations in eight of the currently known nine USH genes, in 49 out of 54 (91%) patients (Table 1). Two or more mutations were identified in 41 patients, including 39 patients (72%) with biallelic mutations, and one mutation was found in the remaining seven patients (13%), that is a total of 81 different mutations. Current diagnostic strategies use a genotyping microarray based on the arrayed primer extension method [24]. Were the international USH genotyping microarray used to identify the mutations, only 30 out of the 81 mutations (37%) would have been possibly detected because of the high prevalence of novel mutations, whatever the USH clinical type. Only 9 mutations previously reported as recurrent were detected in our series of patients (i.e. 11% of the mutations), specifically, c.1996C>T, c.223delG, c.1556G>A, c.494C>T, c.3719G>A and c.5749G>T in MYO7A, c.238_239dupC in USH1C, and c.2299delG and c.10712C>T in USH2A. Therefore, in the process of designing any strategy for USH molecular diagnosis, taking into account the high prevalence of novel mutations appears to be of major importance.

Previous mutation research studies performed in patients referred to medical genetic clinics showed high proportions of mutations for MYO7A, CDH23 and PCDH15 in USH1 patients [27], specifically, 29%-55% for MYO7A[61–64], 19%-35% for CDH23[58], 11%-15% for PCDH15[65], and for USH2A in USH2 patients [28, 60, 66], whereas the implication of VLGR1 and WHRN in the latter was minor [11, 12]. The present analysis confirms these results by showing a major implication of MYO7A in USH1 (55% of the cases), and of USH2A in USH2 (62% of the cases).

Surprisingly, mutations were found in genes that did not fit the clinically diagnosed USH type. None of the six patients diagnosed as USH3 on the basis of the postlingual onset and progressive nature of the deafness, and the absence of vestibular dysfunction (see Subjects and methods) carried a mutation in USH3A. Yet, mutations in USH2 genes were present in all of them, and with a gene distribution similar to that observed in USH2 patients. This finding, which concerns six out of 24 patients carrying mutations in USH2 genes, calls for a revision of the USH2 clinical features. Along the same line, one patient diagnosed as USH2, because he did not have a vestibular dysfunction, carried biallelic missense mutations in an USH1 gene, MYO7A. The two mutations (p.A457V and p. K269del) affect amino acid residues located in the motor head of myosin VIIa, and have previously been reported in USH1 patients [63]. They may preserve a residual activity of the protein, thus causing less severe hearing, balance and visual impairments. Alternatively, one of these mutations or both might be deleterious for the myosin VIIa activity associated with the ankle-link protein complex that underlies the USH2 phenotype [40], but not with the transient hair bundle lateral-link and tip-link molecular complexes that are involved in USH1 pathogenesis. These phenotype/genotype discrepancies further argue in favor of a comprehensive mutation screening procedure that includes genes seemingly inconsistent with the clinical classification of USH currently in use.

Notably, our study has revealed one case of likely oligogenic inheritance for USH1, involving MYO7A and USH1G, and possibly USH2A. Three cases of digenic inheritance of USH1 have been reported so far [42], all caused by mutations in CDH23 and PCDH15, in agreement with the contribution of cadherin-23 and protocadherin-15 to the hair bundle transient lateral links and tip-links [31, 32, 36, 67–69]. The pathogenicity of the p.T1209A mutation in CDH23[18, 55] is, however, questionable since we found it in five alleles from the control population. The c.5601delAAC mutation in PCDH15, leading to an in frame-deletion of a threonine residue (p.T1868del) [42] within the intracellular domain of the protocadherin-15 CD1 isoform, also warrants a special mention. Three protocadherin-15 isoforms (CD1-3) that differ in their intracytoplasmic regions have been reported [69]. Already two presumably pathogenic mutations (p.M1853L and p.T1868del) [42, 70] have been found in exon 34 that is specific for CD1. Incidentally, the p.T1868del mutation was not only involved in USH1, but has also been found, in homozygous state, in a deaf patient presenting with vestibular arreflexia and without retinitis pigmentosa (C. Bonnet, unpublished). The CD2 isoform(s) of protocadherin-15 make(s) the transient kinociliary links [71], whereas the protocadherin-15 isoforms that make transient interstereocilia links and the tip-links are still unknown. The mutations in exon 34, however, point to an essential biological role of CD1, or of an as yet uncharacterized protocadherin-15 isoform that contains the amino acid sequence encoded by this exon, in the hair cells.

Therefore, even though non-monogenic inheritance of USH appears to be rare, it has to be taken into consideration in the molecular diagnosis strategy. In addition, ten patients had presumably pathogenic mutations in two different USH genes. Seven of them had biallelic mutations in one gene, and carried an additional mutation in a second and, for one of them, a third USH gene. None of these additional mutations were nonsense or frame-shifting mutations, but the conservation of the corresponding amino acid residues in the orthologous genes (ush2a, myo7a, whrn) of Ciona savignyi[72], a cnidarian which is evolutionary distant of about 520 million years from man [73], argues in favor of their pathogenicity (Figure 4). Notably, these mutations were not found in 402 to 666 control alleles from populations of matched geographic origin. A substantial proportion of USH patients thus carry a third, presumably pathogenic mutation which, in some cases, may contribute to worsen the sensory defects resulting from missense mutations present in the "primary" USH gene.

Figure 4
**Interspecies conservation of amino acid residues mutated in patients carrying presumably pathogenic mutations in several USH genes**. Representative stretches of amino acid sequences from each of the USH proteins from various species were aligned, and identical residues highlighted with shading. Residues involved in missense mutations are underlined. Protein ID accession numbers are indicated in parentheses. Orthologs of *MYO7A*, *USH2A* and *WHRN* are present in the cnidarian *Ciona savignyi*; they encode proteins that have 53.5%, 36.5%, and 24.7% (whirlin short isoform) of sequence identity with the human proteins, respectively. Notably, the P1220 residue of myosin VIIa, and the G1301 and C3307 residues of usherin, which are involved in the USH patients' missense mutations, are conserved in *C. savignyi*. Incidentally, all the new *USH2A* missense mutations detected in our series of patients affect residues that are also conserved in this species.

Finally, no mutations were detected in five patients, specifically three USH1 and two USH2 patients. In patient S1823 (USH1), born from consanguineous parents, involvement of any of the nine currently known USH genes could be excluded by segregation analysis of polymorphic markers at the corresponding loci (data not shown). In the four remaining patients, the undetected mutations might still be located in the unexplored promoter regions or intragenic regulatory sequences of these genes, but may also be located in other, still unknown USH genes, as in patient S1823. Indeed, a new locus, USH1H, at chromosome 15q22-23 [74], and three candidate regions for new USH2 genes (2q32, 4q26 and 15q22-23) have been reported [75].

Conclusion

Direct exon sequencing of a set of specific disease genes is a reliable, easy set-up method, which remains less expensive than full exome sequencing in the patients. Based on the high prevalence of private mutations both in USH1 and USH2 patients, the substantial number of cases displaying genotype/phenotype discrepancy, and the presence of additional, presumably pathogenic mutations in a number of patients, we conclude that exon sequencing (including flanking splice sites) of all currently known USH genes is required for proper molecular diagnosis in every USH patient, both in the context of genetic counseling and in the perspective of retinal and cochlear gene therapy. The activity of the USH gene carrying biallelic mutations may indeed turn out to be only partly restored by gene therapy, and the presence of a third mutation in another USH gene may then critically impact on the benefits of the gene therapy. Moreover, as PDZD7[76] has recently been reported to modify the phenotype in patients carrying mutations in USH2A or VLGR1[77], future studies should also take into account modifier genes in the USH exome sequencing strategy.

Notes

List of abbreviations

APEX:: Arrayed Primer EXtension

CDH23:: Cadherin 23

DNA:: DeoxyriboNucleic Acid

ESE:: Exonic Splicing Enhancers

MYO7A:: Myosin VIIa

PCR:: Polymerase Chain Reaction

PCDH15:: Protocadherin 15

SIFT:: Sorting Intolerant From Tolerant

USH:: Usher syndrome

USH1:: USH type I

USH2:: USH type II

USH3:: USH type III

VLGR1:: Very Large G protein-coupled Receptor

WHRN:: Whirlin.

Declarations

Acknowledgements

We thank the patients and their families for participation in the study. We are very grateful to Laurent Abel, Jamila El Baghdadi and Cécile Julier for providing us with control DNA samples from Moroccan and Algerian individuals. We thank Corinne Chauve, Catherine Meunier, France Michel, and Isabelle Sargis for expert technical assistance. We also thank Sophie Bahaban, Anne-Flore Grange, Elizabeth Alden Landis and Patrick Joynt for collaboration. We thank Jean-Louis Mandel for helpful discussions. M.G. was supported by European Commission FP6 Integrated project, EuroHear (LSHG-CT-2004-512063) and C.B. by the French Foundation "Voir et Entendre". This work was supported by Fondation R & G Strittmatter (under the aegis of Fondation de France), FAUN Stiftung (Suchert Foundation), EC-FP7 TREATRUSH (HEALTH-F2-2010-242013), Foundation Fighting Blindness, LHW-Stiftung, Fondation Orange, The Conny-Maeva Charitable Foundation, Genoscope-CNRG project AP2005, and S'entendre Foundation.

Electronic supplementary material

13023_2010_295_MOESM1_ESM.PDF Additional file 1: Figure S1: Sequence alignment of amino acid residues mutated in patients carrying missense mutations in USH1 genes. Representative stretches of amino acid sequences from each of the USH1 proteins in various species were aligned. Identical residues are highlighted with shading. Residues involved in missense mutations are underlined. Figure S2: Sequence alignment of amino acid residues mutated in patients carrying missense mutations in USH2 genes. Representative stretches of amino acid sequences from each of the USH2 proteins in various species were aligned. Identical residues are highlighted with shading. Residues involved in missense mutations are underlined. Orthologs of VLGR1 are not present in the genomes of invertebrates such as C. elegans and drosophila. Figure S3: Missense mutations possibly creating or disrupting a splice site. Representative stretches of amino acid sequences from each of the USH proteins in various species were aligned. Identical residues are highlighted with shading. Residues involved in missense mutations are underlined. Triangles indicate splice sites. Scores for splice sites are obtained by NetGene2 software program. Possible new splice sites are in bold. Figure S4: Segregation analysis of the USH2A mutations in family U51. (PDF 2 MB)

13023_2010_295_MOESM2_ESM.DOC Additional file 2: Table S1. Presumably neutral, isocoding and intronic variants in USH genes. (DOC 410 KB)

Below are the links to the authors’ original submitted files for images.

13023_2010_295_MOESM3_ESM.tiff Authors’ original file for figure 1

13023_2010_295_MOESM4_ESM.tiff Authors’ original file for figure 2

13023_2010_295_MOESM5_ESM.tiff Authors’ original file for figure 3

13023_2010_295_MOESM6_ESM.pdf Authors’ original file for figure 4

Authors’ Affiliations

(1)

Unité de Génétique Médicale, INSERM UMRS 587, Hôpital d'Enfants Armand-Trousseau, Assistance Publique-Hôpitaux de Paris (AP-HP), Paris, France

(2)

Unité de Génétique et Physiologie de l'Audition, INSERM UMRS 587, UPMC, Institut Pasteur, Paris, France

(3)

Service de Biochimie et de Biologie Moléculaire, INSERM UMRS 587, Hôpital d'Enfants Armand-Trousseau, Paris, France

(4)

Centre National de Génotypage, Evry, France

(5)

Centre de Génétique, Hôpital St-Antoine, Lille, France

(6)

Service ORL, Hôpital St-Antoine, Lille, France

(7)

Service de Génétique médicale, Hôpital de Hautepierre, Strasbourg, France

(8)

Service ORL, Hôpital de Hautepierre, Strasbourg, France

(9)

Service de Génétique, Hôtel Dieu, Nantes, France

(10)

Service ORL, CHU Hôtel Dieu, Nantes, France

(11)

Maternité Régionale Adolphe-Pinard, Nancy, France

(12)

Service ORL, Maternité Régionale Adolphe-Pinard, Nancy, France

(13)

Centre de Référence des Maladies Neurogénétiques, Centre Hospitalier Universitaire d'Angers, France

(14)

Service ORL, Centre Hospitalier Universitaire d'Angers, France

(15)

Unité de Génétique Médicale, Hôpital, Dijon, France

(16)

Service ORL, Hôpital, Dijon, France

(17)

Génétique Médicale, Hôtel-Dieu, Clermont-Ferrand, France

(18)

Service ORL, Hôtel-Dieu, Clermont-Ferrand, France

(19)

Centre de Génétique, Hôpital Pellegrin, Bordeaux, France

(20)

Service ORL, Hôpital Pellegrin, Bordeaux, France

(21)

Unité de Génétique Clinique, Hôpital Charles-Nicolle, Rouen, France

(22)

Service ORL Pédiatrique, Hôpital Charles-Nicolle, Rouen, France

(23)

Service de Génétique Médicale, Hôpital de la Timone, Marseille, France

(24)

Service de Génétique Médicale, Hôpital intercommunal de Font-Pré, Toulon La Seyne sur Mer, France

(25)

Departement d'Ophtalmologie et de Médecine Interne, Hôpital de la Salpêtrière, France

(26)

Institut de la Vision, INSERM UMRS 968, UPMC, Paris, France

(27)

CEA, DSV, IG, Genoscope, CNRS-UMR 8030, UEVE, Université d'Evry, Evry, France

(28)

Collège de France, Paris, France

(29)

Service d'ORL et de Chirurgie Cervico-faciale, INSERM UMRS 587, Hôpital d'Enfants Armand-Trousseau, AP-HP, UPMC, Paris, France

(30)

NIDCD, NIH, Bethesda, USA

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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Complete exon sequencing of all known Usher syndrome genes greatly improves molecular diagnosis

Abstract

Background

Methods

Results

Conclusions

Keywords

Background

Subjects and Methods

Subjects

PCR amplification and sequencing

Control DNAs

In silico analysis of sequence variants

Segregation analysis

Mutation nomenclature

Protein Accession numbers

Results

Mutation analysis: high prevalence of novel mutations

Transmission modes: evidence for digenic/oligogenic inheritance in some patients

Discussion

Conclusion

Notes

List of abbreviations

Declarations

Acknowledgements

Authors’ Affiliations

References

Copyright

Orphanet Journal of Rare Diseases

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