Volume 10 Supplement 2

Proceedings of the 1st French-Italian meeting on laminopathies and other nuclear envelope-related diseases

Open Access

The nuclear lamina during human spermiogenesis

Orphanet Journal of Rare Diseases201510(Suppl 2):O22

https://doi.org/10.1186/1750-1172-10-S2-O22

Published: 11 November 2015

The nuclear lamina takes centre stage during spermiogenesis, the post-meiotic phase of spermatogenesis, when haploid round spermatids differentiate into spermatozoa: the acrosome and flagellum develop at opposite nuclear poles, the nucleus elongates and, as the nuclear histones are replaced with protamines, the chromatin condenses, to produce the highly compacted pyriform nucleus of the mature spermatozoa. In rodent spermatids, the nuclear lamina contains lamin B1 and lamin B3 a specific isoform of lamin B2 with a shortened rod domain, and A-type lamins are absent[1, 2], but nothing is known about the structure of the nuclear lamina during human spermiogenesis. We are studying the nuclear lamina during human spermiogenesis. We have shown that the human nuclear lamina contains lamin B1 and, distinct from rodents, lamin B2. We also described a transcript potentially encoding a human lamin B3 that, like its mouse counterpart[3], induces severe nuclear deformation when expressed in HeLa cells[4]. In human, lamin B1 and B2 localise to the nuclear periphery in spermatids except in the region covered by the acrosome. They are seen to recede to the posterior pole of the nucleus as the spermatids progress through spermiogenesis. Lamin B1 was observed on 30-40% of ejaculated spermatozoa, while lamin B2 was not detected. The percentage of B1-labelled spermatozoa dropped at least 6-fold when spermatozoa with normal head density were selected, indicating that lamin B1 labels immature spermatozoa lacking a fully compacted nucleus, and may therefore be a marker of poor sperm quality. The comparison of the human nuclear lamina with that of the mouse suggests that lamin B1 and B3 have critical roles during mammalian spermiogenesis.

Authors’ Affiliations

(1)
Aix Marseille Université, INSERM

References

  1. Schutz W, Alsheimer M, Ollinger R, Benavente R: Nuclear envelope remodeling during mouse spermiogenesis: postmeiotic expression and redistribution of germline lamin B3. Experimental cell research. 2005, 307 (2): 285-91.PubMedView ArticleGoogle Scholar
  2. Vester B, Smith A, Krohne G, Benavente R: Presence of a nuclear lamina in pachytene spermatocytes of the rat. Journal of cell science. 1993, 104 (Pt 2): 557-63.PubMedGoogle Scholar
  3. Schutz W, Benavente R, Alsheimer M: Dynamic properties of germ line-specific lamin B3: the role of the shortened rod domain. European journal of cell biology. 2005, 84 (7): 649-62.PubMedView ArticleGoogle Scholar
  4. Elkhatib R, Longepied G, Paci M, Achard V, Grillo JM, Levy N, et al: Nuclear envelope remodelling during human spermiogenesis involves somatic B-type lamins and a spermatid-specific B3 lamin isoform. Molecular human reproduction. 2015, 21 (3): 225-36.PubMedView ArticleGoogle Scholar

Copyright

© Mitchell 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Advertisement