Volume 10 Supplement 2
Altered cytokine profiles in laminopathic patients
© Bernasconi 2015
Published: 11 November 2015
Prelamin A accumulation is known to dysregulate the NF-κB signaling cascade, causing a secretion of high levels of proinflammatory cytokines, which in turn might contribute to the pathologic aging observed in laminopathies, and in particular in HGPS. In collaboration with researchers and clinicians of the Italian network for Laminopathies, we wondered whether it was possible to identify a pattern of cytokine expression that could discriminate laminopathy from other forms of muscular dystrophy and/or cardiomyopathy and a laminopathy with a cardiac involvement from one with only muscle involvement, with the final goal to identify biomarker(s) helpful for diagnosis, prognosis and evaluation of therapy efficacy. We analysed the cytokine profiles of sera collected from 37 patients affected by different forms of laminopathy (all LMNA mutations), 9 patients affected by genetically defined non-LMNA muscular dystrophy and 27 healthy individuals. Sera were screened for the expression levels of 16 cytokines, 6 chemokines, 5 growth factors and TGF-beta1, 2 and 3 by Luminex technology. Some pro-inflammatory cytokines were found to be differentially expressed in cardiopathic and non-cardiopathic patients compared to healthy controls, and among laminopathies with muscle and cardiac involvement, laminopathies without myopathy and muscular dystrophies. Interestingly, TGF-beta2 serum levels were higher in the LMNA patients than in healthy individuals and in patients with non-LMNA muscular dystrophy, suggesting a direct link between LMNA mutations and dysregulation of TGFbeta2 pathway, as indicated by previous and recent experimental studies[2, 3].
- Osorio FG, Barcena C, Soria-Valles C, Ramsay AJ, de Carlos F, Cobo J, et al: Nuclear lamina defects cause ATM-dependent NF-kappaB activation and link accelerated aging to a systemic inflammatory response. Genes & development. 2012, 26 (20): 2311-24.View ArticleGoogle Scholar
- Avnet S, Pallotta R, Perut F, Baldini N, Pittis MG, Saponari A, et al: Osteoblasts from a mandibuloacral dysplasia patient induce human blood precursors to differentiate into active osteoclasts. Biochimica et biophysica acta. 2011, 1812 (7): 711-8.PubMedView ArticleGoogle Scholar
- Evangelisti C, Bernasconi P, Cavalcante P, Cappelletti C, D'Apice MR, Sbraccia P, et al: Modulation of TGFbeta 2 levels by lamin A in U2-OS osteoblast-like cells: understanding the osteolytic process triggered by altered lamins. Oncotarget. 2015, 6 (10): 7424-37.PubMedPubMed CentralView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.