Impairment of Lamin A/C-Polycomb crosstalk as a possible epigenetic cause of Emery Dreifuss Muscular Dystrophy (EDMD)
© Lanzuolo 2015
Published: 11 November 2015
Traditionally, studies on EDMD have focused on genetic changes affecting molecules involved in the development of pathology. However, emerging findings indicate that a single genetic mutation can be accompanied by a range of different phenotypes, suggesting a contribution of the epigenetic background to the disease progression. This is in line with recent works showing that changes in chromatin architecture are peculiar of several laminopathies[1, 2]. Despite much effort has been done to understand the regulation of the complex networks of gene expression that govern muscle differentiation and that is affected in EDMD, little is known about the epigenetic players and molecular mechanisms involved in pathogenesis and progression. Key epigenetic regulators of chromatin architecture are Polycomb group (PcG) of proteins, epigenetic transcriptional repressors of genes primarily involved in differentiation and development. In particular, during myogenesis, modulation of Ezh2 levels, the catalytic subunit of the Polycomb Repressive Complex 2 (PRC2) ensures the correct muscle differentiation. In the nucleus, PcG proteins form microscopically visible foci and high-through-put data together with microscopy analysis revealed that their targets are organized in chromatin loops[5, 6]. We have shown that Lamin A/C sustains PcG foci influencing PcG nuclear compartmentalization and modulating their repressive functions. During myogenesis, Lamin A/C depletion leads to an altered timing of muscle differentiation due to the aberrant expression of PcG-regulated genes.
- McCord RP, Nazario-Toole A, Zhang H, Chines PS, Zhan Y, Erdos MR, et al: Correlated alterations in genome organization, histone methylation, and DNA-lamin A/C interactions in Hutchinson-Gilford progeria syndrome. Genome research. 2013, 23 (2): 260-9.PubMedPubMed CentralView ArticleGoogle Scholar
- Shumaker DK, Dechat T, Kohlmaier A, Adam SA, Bozovsky MR, Erdos MR, et al: Mutant nuclear lamin A leads to progressive alterations of epigenetic control in premature aging. Proceedings of the National Academy of Sciences of the United States of America. 2006, 103 (23): 8703-8.PubMedPubMed CentralView ArticleGoogle Scholar
- Lanzuolo C, Orlando V: Memories from the polycomb group proteins. Annual review of genetics. 2012, 46: 561-89.PubMedView ArticleGoogle Scholar
- Caretti G, Di Padova M, Micales B, Lyons GE, Sartorelli V: The Polycomb Ezh2 methyltransferase regulates muscle gene expression and skeletal muscle differentiation. Genes & development. 2004, 18 (21): 2627-38.View ArticleGoogle Scholar
- Bantignies F, Roure V, Comet I, Leblanc B, Schuettengruber B, Bonnet J, et al: Polycomb-dependent regulatory contacts between distant Hox loci in Drosophila. Cell. 2011, 144 (2): 214-26.PubMedView ArticleGoogle Scholar
- Lanzuolo C, Roure V, Dekker J, Bantignies F, Orlando V: Polycomb response elements mediate the formation of chromosome higher-order structures in the bithorax complex. Nature cell biology. 2007, 9 (10): 1167-74.PubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.