- Open Access
Bernard-Soulier syndrome (Hemorrhagiparous thrombocytic dystrophy)
© Lanza; licensee BioMed Central Ltd. 2006
- Received: 10 October 2006
- Accepted: 16 November 2006
- Published: 16 November 2006
Bernard-Soulier syndrome (BSS), also known as Hemorrhagiparous thrombocytic dystrophy, is a hereditary bleeding disorder affecting the megakaryocyte/platelet lineage and characterized by bleeding tendency, giant blood platelets and low platelet counts. This syndrome is extremely rare as only ~100 cases have been reported in the literature. Clinical manifestations usually include purpura, epistaxis, menorrhagia, gingival and gastrointestinal bleeding. The syndrome is transmitted as an autosomal recessive trait. The underlying defect is a deficiency or dysfunction of the glycoprotein GPIb-V-IX complex, a platelet-restricted multisubunit receptor required for normal primary hemostasis. The GPIb-V-IX complex binds von Willebrand factor, allowing platelet adhesion and platelet plug formation at sites of vascular injury. Genes coding for the four subunits of the receptor, GPIBA, GPIBB, GP5 and GP9, map to chromosomes 17p12, 22q11.2, 3q29, and 3q21, respectively. Defects have been identified in GPIBA, GPIBB, and GP9 but not in GP5. Diagnosis is based on a prolonged skin bleeding time, the presence of a small number of very large platelets (macrothrombocytopenia), defective ristocetin-induced platelet agglutination and low or absent expression of the GPIb-V-IX complex. Prothrombin consumption is markedly reduced. The prognosis is usually good with adequate supportive care but severe bleeding episodes can occur with menses, trauma and surgical procedures. Treatment of bleeding or prophylaxis during surgical procedures usually requires platelet transfusion.
- Autosomal Recessive Disease
- Autosomal Recessive Trait
- Large Platelet
- Decrease Surface Expression
- Severe Bleeding Episode
Bernard-Soulier syndrome (BSS)
Hemorrhagiparous thrombocytic dystrophy
Congenital hemorrhagiparous thrombocytic dystrophy
The Bernard-Soulier syndrome (BSS) is an autosomal recessive disease associated with bleeding tendency, giant blood platelets and low platelet counts. The defect is restricted to the megakaryocyte/platelet lineage.
Congenital platelet disorders are often difficult to distinguish on the basis of clinical manifestations and require specialized laboratory tests. Functional analysis of platelet suspensions by aggregometry is needed to differentiate BSS from other rare inherited disorders accompanied by macrothrombocytopenia, such as the May-Hegglin, Sebastian, Fechtner and Epstein's syndromes . A firm diagnosis calls for conjunction of increased bleeding times, macrothrombocytopenia, defective ristocetin-induced agglutination and low or absent levels of platelet GPIb-V-IX (CD42a-d) by flow cytometry.
This syndrome is extremely rare as only ~100 cases have been reported in published articles, mostly in the populations of Japan, Europe, and North America. Prevalence has been estimated at less than 1/1,000,000 but is probably higher due to misdiagnosis and underreporting. This low frequency could probably be explained by the fact that the affected genes are very compact being interrupted by only 1 or 2 introns.
In 1948, Jean Bernard and Jean-Pierre Soulier , two French hematologists, described a young male patient who had a severe bleeding defect with a prolonged bleeding time, a low platelet count with very large platelets (macrothrombocytopenia). In view of these defects, they named the disorder "Dystrophie thrombocytaire-hémorragipare congénitale" (Hemorrhagiparous thrombocytic dystrophy). Additional cases presenting with an identical disorder, mostly transmitted as an autosomal recessive trait and often associated with consanguinity, have been subsequently reported [3, 4]. In the literature, the disease is now commonly referred to as Bernard-Soulier syndrome (BSS). In most cases, bleeding symptoms manifest rapidly after birth or during early childhood. Clinical manifestations usually include purpura, epistaxis, gingival bleeding and menorrhagia, and more rarely gastrointestinal bleeding and hematuria. Severe bleeding episodes are associated with trauma and surgical procedures such as tonsillectomy, appendectomy, splenectomy, or occur during dental extractions and menses. However, the severity and frequency of bleeding vary between individuals. Bleeding mainly affects mucocutaneous tissues, and major hematomas are very rarely observed.
To date, 47 different genetic defects associated to the BSS have been identified [see Additional file 1]. A Bernard-Soulier registry can also be found at Bernard-Soulier syndrome web site . Defects are due to mutations in GPIBA (20 mutations), which is the largest subunit and bears the von Willebrand binding site, in GPIBB (16 mutations) and in GP9 (11 mutations). The defects can be separated into three major classes: 1) missense mutations or short in-frame deletions which rarely give rise to normal or slightly decreased expression of a dysfunctional receptor or more often to an abnormal/unstable complex with strongly decreased surface expression; 2) nonsense mutations resulting in smaller subunits that typically lack the transmembrane domain; 3) frameshift insertions or deletions leading to a novel polypeptide sequence and often to a premature stop. In very few cases, a single mutation, like in some GPIbα and GPIX mutants, affects mainly the function of the receptor, which is at least partially expressed in giant platelets. Most of the defects are unique to a single individual or family. One exception is the GP9 Asn45Ser mutation, which has been reported in several families from different countries, probably as a result of a founder effect . The Ala156Val mutation also occurs frequently in the Italian population, with an apparent dominant transmission leading to macrothrombocytopenia in heterozygote individuals . Several mutations combining a BSS phenotype and the DiGeorge/Velo-cardio-facial syndrome (VCF) have been reported. In these cases, one allele carried a mutated GPIBB gene, while the other allele had the large deletion of the chromosomal region 22q11.2, containing the GPIBB gene, typical of the Di George/VCF defect .
Several polymorphisms have been reported especially in the GPIBA gene. They include silent mutations, a missense mutation which gives rise to an alloantigen and a variable number of tandem repeat (VNTR) polymorphism . To date, none of these polymorphisms have been linked to anomalies in receptor expression or function.
Rare gain of function mutations of GPIBA give rise to platelet-type von Willebrand disease, a clinical entity distinct from BSS. The condition resembles type IIB von Willebrand disease due to clearance of plasma von Willebrand factor by aggregated platelets. Three missense mutations in a disulfide loop flanking the leucine-rich domain and a 9 amino acid deletion in the macroglycopeptide have been reported in these patients [21–24].
Skin bleeding times in BSS are moderately (5–10 min) to severely (>20 min) prolonged. A constant feature is the presence of a small number of very large platelets with a rounded shape (main volume 11–16 μ m3; diameter 4–10 μ m). The initial laboratory test should therefore include blood cell counts and examination of blood smears. Platelet counts typically range from 20,000 to 100,000/μ l. Manual counting is required for an accurate determination as the very large platelets in BSS are often mistaken for lymphocytes in automatic counters. The distinctive abnormality of BSS platelets is an isolated defect in ristocetin-induced agglutination. Unlike the defect in von Willebrand disease, this anomaly is not corrected by the addition of normal plasma. Levels of FVIII-von Willebrand complex are assessed. Aggregation responses to agonists such as ADP or collagen are normal, however decreased responses to thrombin can be observed. A marked defect in prothrombin consumption is constantly observed and may be useful for the diagnosis: it is attributed to a defective binding of FXI due to a lack of GPIb , and to a decrease in GPIb-fibrin-dependent thrombin generation [26, 27]. Flow cytometry analysis using a panel of specific monoclonal antibodies (CD42 a-d) will confirm this diagnosis. Additional tests in specialized research units may include platelet glycoprotein analysis by SDS-polyacrylamide gel separation and immunoblotting, and finally, study of genetic abnormalities.
BSS belongs to a heterogeneous group of rare inherited diseases characterized by a reduced number of platelets (thrombocytopenia). A published diagnostic algorithm should facilitate differential diagnosis of BSS . For example, presence of small platelets is typical of Wiscott-Aldrich syndrome and X-linked thrombocytopenia. Giant platelets associated with neutrophil inclusions will orient toward MYH9-related diseases (May-Hegglin, Sebastian, Fechtner, and Epstein syndromes). Other parameters will help diagnose macrothrombocytopenia in Di George, Gray platelet, Montreal and Paris-Trousseau syndromes. Finally, some cases of heterozygous BSS can also exhibit macrothrombocytopenia.
BSS is difficult to be distinguished on the basis of clinical manifestations only and has often been misdiagnosed as idiopathic thrombocytopenic purpura (ITP), an immunological disorder, and treated unsuccessfully with steroids or splenectomy.
Genetic counseling should follow the standards established for all autosomal recessive diseases.
Prenatal diagnosis is theoretically feasible when the genetic defect has been identified in a particular kindred. This is probably not justified as the syndrome rarely gives rise to life threatening bleeding. With a good prophylaxis, a fairly normal quality of life can be maintained. In addition, cord blood or chorion villus sampling bear a high risk of bleeding and premature abortion.
Therapeutic approaches include both general and specific treatment of bleeding. Patients should be warned to avoid traumas, antiplatelet medication such as aspirin, to maintain adequate dental hygiene and to use contraceptive in female at puberty. Treatment of bleeding or prophylaxis during surgical procedures usually requires blood or platelet transfusion with the associated risk of developing antiplatelet alloantibodies. Desmopressin and rFVIIa administration have been shown to shorten the bleeding time in some patients. In rare cases of patients with life-threatening disorders, bone-marrow or umbilical-cord hematopoietic stem cell transplantation may be considered . As a rather simple genetic defect, BSS could be a candidate for future gene replacement therapy using virally transduced megakaryocyte progenitors.
With a good education, adequate care and prevention of trauma, patients can live a fairly normal life. Nevertheless, possible occurrence of severe bleeding in case of trauma and surgical intervention should always be kept in mind and treated adequately.
To date, no BSS patient has been identified with a mutation of the GPV subunit but existence of such defects cannot be ruled out, and they could potentially result in variant or mild forms of BSS.
As for other platelet deficiencies, it is difficult to correlate genetic defects with propensity to bleed and severity of bleeding.
The molecular and cellular mechanisms responsible for the several platelet defects encountered in BSS are still largely unknown. An explanation for their enlarged platelets and low platelet counts is probably related to defective megakaryocytopoiesis but such studies are difficult to conduct in BSS patients. Additionally, platelet functional studies in vivo and evaluation of a putative protection against thrombosis are not amenable in BSS patients. Finally, there is no clear explanation for the abnormal prothrombin consumption in this pathology. Mouse models of BSS are now available with deletions of the GPIbα or GPIbβ subunit which should help answering these remaining questions.
- Nurden AT: Qualitative disorders of platelets and megakaryocytes. J Thromb Haemost. 2005, 3: 1773-1782. 10.1111/j.1538-7836.2005.01428.x.View ArticlePubMedGoogle Scholar
- Bernard J, Soulier JP: Sur une nouvelle variété de dystrophie thrombocytaire-hémorragipare congénitale. Sem Hop Paris. 1948, 24: 3217-3222.PubMedGoogle Scholar
- de la Salle C, Lanza F, Cazenave JP: Biochemical and molecular basis of Bernard-Soulier syndrome: a review. Nouv Rev Fr Hematol. 1995, 37: 215-222.PubMedGoogle Scholar
- Lopez JA, Andrews RK, Afshar-Kharghan V, Berndt MC: Bernard-Soulier syndrome. Blood. 1998, 91: 4397-4418.PubMedGoogle Scholar
- Clemetson KJ: Platelet receptors and their role in diseases. Clin Chem Lab Med. 2003, 41: 253-260. 10.1515/CCLM.2003.039.View ArticlePubMedGoogle Scholar
- Ruggeri ZM: Von Willebrand factor, platelets and endothelial cell interactions. J Thromb Haemost. 2003, 1: 1335-1343. 10.1046/j.1538-7836.2003.00260.x.View ArticlePubMedGoogle Scholar
- Andrews RK, Shen Y, Gardiner EE, Dong JF, Lopez JA, Berndt MC: The glycoprotein Ib-IX-V complex in platelet adhesion and signaling. Thromb Haemost. 1999, 82: 357-364.PubMedGoogle Scholar
- Ulsemer P, Strassel C, Baas MJ, Salamero J, Chasserot-Golaz S, Cazenave JP, de la Salle C, Lanza F: Biosynthesis and intracellular post-translational processing of normal and mutant platelet glycoprotein GPIb-IX. Biochem J. 2001, 358: 295-303. 10.1042/0264-6021:3580295.PubMed CentralView ArticlePubMedGoogle Scholar
- Lopez JA, Chung DW, Fujikawa K, Hagen FS, Papayannopoulou T, Roth GJ: Cloning of the alpha chain of human platelet glycoprotein Ib: a transmembrane protein with homology to leucine-rich alpha 2-glycoprotein. Proc Natl Acad Sci USA. 1987, 84: 5615-5619. 10.1073/pnas.84.16.5615.PubMed CentralView ArticlePubMedGoogle Scholar
- Wenger RH, Wicki AN, Kieffer N, Adolph S, Hameister H, Clemetson KJ: The 5' flanking region and chromosomal localization of the gene encoding human platelet membrane glycoprotein Ib alpha. Gene. 1989, 85: 517-524. 10.1016/0378-1119(89)90446-0.View ArticlePubMedGoogle Scholar
- Lopez JA, Chung DW, Fujikawa K, Hagen FS, Davie EW, Roth GJ: The alpha and beta chains of human platelet glycoprotein Ib are both transmembrane proteins containing a leucine-rich amino acid sequence. Proc Natl Acad Sci USA. 1988, 85: 2135-2139. 10.1073/pnas.85.7.2135.PubMed CentralView ArticlePubMedGoogle Scholar
- Yagi M, Edelhoff S, Disteche CM, Roth GJ: Structural characterization and chromosomal location of the gene encoding human platelet glycoprotein Ib beta. J Biol Chem. 1994, 269: 17424-17427.PubMedGoogle Scholar
- Lanza F, Morales M, de la Salle C, Cazenave JP, Clemetson KJ, Shimomura T, Phillips DR: Cloning and characterization of the gene encoding the human platelet glycoprotein V. A member of the leucine-rich glycoprotein family cleaved during thrombin-induced platelet activation. J Biol Chem. 1993, 268: 20801-20807.PubMedGoogle Scholar
- Hickey MJ, Deaven LL, Roth GJ: Human platelet glycoprotein IX. Characterization of cDNA and localization of the gene to chromosome 3. FEBS Lett. 1990, 274: 189-192. 10.1016/0014-5793(90)81361-Q.View ArticlePubMedGoogle Scholar
- Yagi M, Edelhoff S, Disteche CM, Roth GJ: Human platelet glycoproteins V and IX: mapping of two leucine-rich glycoprotein genes to chromosome 3 and analysis of structures. Biochemistry. 1995, 34: 16132-16137. 10.1021/bi00049a028.View ArticlePubMedGoogle Scholar
- Bernard-Soulier Syndrome. [http://www.bernardsoulier.org]
- Liang HP, Morel-Kopp MC, Clemetson JM, Clemetson KJ, Kekomaki R, Kroll H, Michaelides K, Tuddenham EG, Vanhoorelbeke K, Ward CM: A common ancestral glycoprotein (GP) 9 1828A>G (Asn45Ser) gene mutation occurring in European families from Australia and Northern Europe with Bernard-Soulier Syndrome (BSS). Thromb Haemost. 2005, 94: 599-605.PubMedGoogle Scholar
- Savoia A, Balduini CL, Savino M, Noris P, Del Vecchio M, Perrotta S, Belletti S, Poggi , Iolascon A: Autosomal dominant macrothrombocytopenia in Italy is most frequently a type of heterozygous Bernard-Soulier syndrome. Blood. 2001, 97: 1330-1305. 10.1182/blood.V97.5.1330.View ArticlePubMedGoogle Scholar
- Budarf ML, Konkle BA, Ludlow LB, Michaud D, Li M, Yamashiro DJ, McDonald-McGinn D, Zackai EH, Driscoll DA: Identification of a patient with Bernard-Soulier syndrome and a deletion in the DiGeorge/velo-cardio-facial chromosomal region in 22q11.2. Hum Mol Genet. 1995, 4: 763-766.View ArticlePubMedGoogle Scholar
- Lopez JA, Ludwig EH, McCarthy BJ: Polymorphism of human glycoprotein Ib alpha results from a variable number of tandem repeats of a 13-amino acid sequence in the mucin-like macroglycopeptide region. Structure/function implications. J Biol Chem. 1992, 267: 10055-10061.PubMedGoogle Scholar
- Miller JL, Cunningham D, Lyle VA, Finch CN: Mutation in the gene encoding the alpha chain of platelet glycoprotein Ib in platelet-type von Willebrand disease. Proc Natl Acad Sci USA. 1991, 88: 4761-4765. 10.1073/pnas.88.11.4761.PubMed CentralView ArticlePubMedGoogle Scholar
- Russell SD, Roth GJ: Pseudo-von Willebrand disease: a mutation in the platelet glycoprotein Ib alpha gene associated with a hyperactive surface receptor. Blood. 1993, 81: 1787-1791.PubMedGoogle Scholar
- Matsubara Y, Murata M, Sugita K, Ikeda Y: Identification of a novel point mutation in platelet glycoprotein Ibalpha, Gly to Ser at residue 233, in a Japanese family with platelet-type von Willebrand disease. J Thromb Haemost. 2003, 1: 2198-2205. 10.1046/j.1538-7836.2003.00369.x.View ArticlePubMedGoogle Scholar
- Othman M, Notley C, Lavender FL, White H, Byrne CD, Lillicrap D, O'Shaughnessy DF: Identification and functional characterization of a novel 27-bp deletion in the macroglycopeptide-coding region of the GPIBA gene resulting in platelet-type von Willebrand disease. Blood. 2005, 105: 4330-4336. 10.1182/blood-2002-09-2942.View ArticlePubMedGoogle Scholar
- Baglia FA, Badellino KO, Li CQ, Lopez JA, Walsh PN: Factor XI binding to the platelet glycoprotein Ib-IX-V complex promotes factor XI activation by thrombin. J Biol Chem. 2002, 18: 1662-1668. 10.1074/jbc.M108319200.View ArticleGoogle Scholar
- Beguin S, Kumar R, Keularts I, Seligsohn U, Coller BS, Hemker HC: Fibrin-dependent platelet procoagulant activity requires GPIb receptors and von Willebrand factor. Blood. 1999, 93: 564-570.PubMedGoogle Scholar
- Al Dieri R, Bellucci S, Beguin S, Hemker HC, Caen J: Defective vWF-Fibrin-GPIb interaction causes impaired prothrombin consumption in Bernard-Soulier Syndrome [abstract]. J Thromb Haemost. 2003, 754.Google Scholar
- Balduini CL, Cattaneo M, Fabris F, Gresele P, Iolascon A, Pulcinelli FM, Savoia A: Inherited thrombocytopenias: a proposed diagnostic algorithm from the Italian Gruppo di Studio delle Piastrine. Haematologica. 2003, 88: 582-592.PubMedGoogle Scholar
- Locatelli F, Rossi G, Balduini C: Hematopoietic stem-cell transplantation for the Bernard-Soulier syndrome. Ann Intern Med. 2003, 138: 79.View ArticlePubMedGoogle Scholar
- Kunishima S, Imai T, Hamaguchi M, Saito H: Novel heterozygous missense mutation in the second leucine rich repeat of GPIba affects GPIb/IX/V expression and results in macrothrombocytopenia in a patient initially misdiagnosed with idiopathic thrombocytopenic purpura. Eur J Haematol. 2006, 76: 348-355. 10.1111/j.1600-0609.2005.00612.x.View ArticlePubMedGoogle Scholar
- Miller JL, Lyle VA, Cunningham D: Mutation of leucine-57 to phenylalanine in a platelet glycoprotein Ib alpha leucine tandem repeat occurring in patients with an autosomal dominant variant of Bernard-Soulier disease. Blood. 1992, 79: 439-446.PubMedGoogle Scholar
- Kenny D, Jonsson OG, Morateck PA, Montgomery RR: Naturally occurring mutations in glycoprotein Ib that result in defective ligand binding and synthesis of a truncated protein. Blood. 1998, 92: 175-183.PubMedGoogle Scholar
- Li C, Martin SE, Roth GJ: The genetic defect in two well-studied cases of Bernard-Soulier syndrome: a point mutation in the fifth leucine-rich repeat of platelet glycoprotein Ib alpha. Blood. 1995, 86: 3805-3814.PubMedGoogle Scholar
- Antonucci JV, Martin ES, Hulick PJ, Joseph A, Martin SE: Bernard-Soulier syndrome: Common ancestry in two African American families with the GP Ib Leu129Pro mutation. Am J Hematol. 2000, 65: 141-148. 10.1002/1096-8652(200010)65:2<141::AID-AJH9>3.0.CO;2-H.View ArticlePubMedGoogle Scholar
- Koskela S, Partanen J, Salmi TT, Kekomaki R: Molecular characterization of two mutations in platelet glycoprotein (GP) Ib alpha in two Finnish Bernard-Soulier syndrome families. Eur J Haematol. 1999, 62: 160-168.View ArticlePubMedGoogle Scholar
- Ware J, Russell SR, Marchese P, Murata M, Mazzucato M, De Marco L, Ruggeri ZM: Point mutation in a leucine-rich repeat of platelet glycoprotein Ib alpha resulting in the Bernard-Soulier syndrome. J Clin Invest. 1993, 92: 1213-1220.PubMed CentralView ArticlePubMedGoogle Scholar
- Margaglione M, D'Andrea G, Grandone E, Brancaccio V, Amoriello A, Di Minno G: Compound heterozygosity (554–589 del, C515-T transition) in the platelet glycoprotein Ib alpha gene in a patient with a severe bleeding tendency. Thromb Haemost. 1999, 81: 486-492.PubMedGoogle Scholar
- de la Salle C, Baas MJ, Lanza F, Schwartz A, Hanau D, Chevalier J, Gachet C, Briquel ME, Cazenave JP: A three-base deletion removing a leucine residue in a leucine-rich repeat of platelet glycoprotein Ib alpha associated with a variant of Bernard-Soulier syndrome (Nancy I). Br J Haematol. 1995, 89: 386-396.View ArticlePubMedGoogle Scholar
- Ulsemer P, Lanza F, Baas MJ, Schwartz A, Ravanat C, Briquel ME, Cranmer S, Jackson S, Cazenave JP, de la Salle C: Role of the leucine-rich domain of platelet GPIbalpha in correct post-translational processing – the Nancy I Bernard-Soulier mutation expressed on CHO cells. Thromb Haemost. 2000, 84: 104-111.PubMedGoogle Scholar
- Simsek S, Noris P, Lozano M, Pico M, von dem Borne AE, Ribera A, Gallardo D: Cys209 Ser mutation in the platelet membrane glycoprotein Ib alpha gene is associated with Bernard-Soulier syndrome. Br J Haematol. 1994, 88: 839-844.View ArticlePubMedGoogle Scholar
- Gonzalez-Manchon C, Larrucea S, Pastor AL, Butta N, Arias-Salgado EG, Ayuso MS, Parrilla R: Compound heterozygosity of the GPIbalpha gene associated with Bernard-Soulier syndrome. Thromb Haemost. 2001, 86: 1385-1391.PubMedGoogle Scholar
- Ware J, Russell SR, Vicente V, Scharf RE, Tomer A, McMillan R, Ruggeri ZM: Nonsense mutation in the glycoprotein Ib alpha coding sequence associated with Bernard-Soulier syndrome. Proc Natl Acad Sci USA. 1990, 87: 2026-2030. 10.1073/pnas.87.5.2026.PubMed CentralView ArticlePubMedGoogle Scholar
- Kunishima S, Miura H, Fukutani H, Yoshida H, Osumi K, Kobayashi S, Ohno R, Naoe T: Bernard-Soulier syndrome Kagoshima: Ser 444-->stop mutation of glycoprotein (GP) Ib alpha resulting in circulating truncated GPIb alpha and surface expression of GPIb beta and GPIX. Blood. 1994, 84: 3356-3362.PubMedGoogle Scholar
- Holmberg L, Karpman D, Nilsson I, Olofsson T: Bernard-Soulier syndrome Karlstad: Trp 498-->Stop mutation resulting in a truncated glycoprotein Ib alpha that contains part of the transmembranous domain. Br J Haematol. 1997, 98: 57-63. 10.1046/j.1365-2141.1997.1772993.x.View ArticlePubMedGoogle Scholar
- Li C, Pasquale DN, Roth GJ: Bernard-Soulier syndrome with severe bleeding: absent platelet glycoprotein Ib alpha due to a homozygous one-base deletion. Thromb Haemost. 1996, 76: 670-674.PubMedGoogle Scholar
- Afshar-Kharghan V, Craig FE, Lopez JA: Bernard-Soulier syndrome in a patient doubly heterozygous for two frameshift mutations in the glycoprotein ib alpha gene. Br J Haematol. 2000, 110: 919-924. 10.1046/j.1365-2141.2000.02261.x.View ArticlePubMedGoogle Scholar
- Simsek S, Noris P, Lozano M, Pico M, von dem Borne AE, Ribera A, Gallardo D: Cys209 Ser mutation in the platelet membrane glycoprotein Ib alpha gene is associated with Bernard-Soulier syndrome. Br J Haematol. 1994, 88: 839-844.View ArticlePubMedGoogle Scholar
- Kanaji T, Okamura T, Kurolwa M, Noda M, Fujimura K, Kuramoto A, Sano M, Nakano S, Niho Y: Molecular and genetic analysis of two patients with Bernard-Soulier syndrome – identification of new mutations in glycoprotein Ib alpha gene. Thromb Haemost. 1997, 77: 1055-1061.PubMedGoogle Scholar
- Noda M, Fujimura K, Takafuta T, Shimomura T, Fujimoto T, Yamamoto N, Tanoue K, Arai M, Suehiro A, Kakishita E, Shimasaki A, Kuramoto A: Heterogeneous expression of glycoprotein Ib, IX and V in platelets from two patients with Bernard-Soulier syndrome caused by different genetic abnormalities. Thromb Haemost. 1995, 74: 1411-1415.PubMedGoogle Scholar
- Noda M, Fujimura K, Takafuta T, Shimomura T, Fujii T, Katsutani S, Fujimoto T, Kuramoto A, Yamazaki T, Mochizuki T, Matsuzaki M, Sano M: A point mutation in glycoprotein IX coding sequence (Cys73 (TGT) to Tyr(TAT)) causes impaired surface expression of GPIb/IX/V complex in two families with Bernard-Soulier syndrome. Thromb Haemost. 1996, 76: 874-878.PubMedGoogle Scholar
- Mitsui T, Yokoyama S, Yazaki N, Hayashi T, Suzuki K, Shimizu Y, Kawakami T, Kanazawa C, Katsuura M, Ikegami T, Hayasaka K: Severe bleeding tendency in a patient with Bernard-Soulier syndrome associated with a homozygous single base pair deletion in the gene coding for the human platelet glycoprotein Ibalpha. J Pediatr Hematol Oncol. 1998, 20: 246-251. 10.1097/00043426-199805000-00011.View ArticlePubMedGoogle Scholar
- Afshar-Kharghan V, Lopez JA: Bernard-Soulier syndrome caused by a dinucleotide deletion and reading frameshift in the region encoding the glycoprotein Ib alpha transmembrane domain. Blood. 1997, 90: 2634-2643.PubMedGoogle Scholar
- Kenny D, Newman PJ, Morateck PA, Montgomery RR: A dinucleotide deletion results in defective membrane anchoring and circulating soluble glycoprotein Ib alpha in a novel form of Bernard-Soulier syndrome. Blood. 1997, 90: 2626-2633.PubMedGoogle Scholar
- Gonzalez-Manchon C, Butta N, Iruin G, Alonso S, Ayuso MS, Parrilla R: Disruption of the Cys5–Cys7 disulfide bridge in the platelet glycoprotein Ibbeta prevents the normal maturation and surface exposure of GPIb-IX complexes. Thromb Haemost. 2003, 90: 456-464.PubMedGoogle Scholar
- Kunishima S, Naoe T, Kamiya T, Saito H: Novel heterozygous missense mutation in the platelet glycoprotein Ib beta gene associated with isolated giant platelet disorder. Am J Hematol. 2001, 68: 249-255. 10.1002/ajh.10000.View ArticlePubMedGoogle Scholar
- Hillmann A, Nurden A, Nurden P, Combrie R, Claeyssens S, Moran N, Kenny D: A novel hemizygous Bernard-Soulier Syndrome (BSS) mutation in the amino terminal domain of glycoprotein (GP) Ibbeta – platelet characterization and transfection studies. Thromb Haemost. 2002, 88: 1026-1032.PubMedGoogle Scholar
- Strassel C, Pasquet JM, Alessi MC, Juhan-Vague I, Chambost H, Combrie R, Nurden P, Bas MJ, De La Salle C, Cazenave JP, Lanza F, Nurden AT: A novel missense mutation shows that GPIbbeta has a dual role in controlling the processing and stability of the platelet GPIb-IX adhesion receptor. Biochemistry. 2003, 42: 4452-4462. 10.1021/bi026213d.View ArticlePubMedGoogle Scholar
- Kunishima S, Tomiyama Y, Honda S, Fukunishi M, Hara J, Inoue C, Kamiya T, Saito H: Homozygous Pro74-->Arg mutation in the platelet glycoprotein Ibbeta gene associated with Bernard-Soulier syndrome. Thromb Haemost. 2000, 84: 112-117.PubMedGoogle Scholar
- Kurokawa Y, Ishida F, Kamijo T, Kunishima S, Kenny D, Kitano K, Koike K: A missense mutation (Tyr88 to Cys) in the platelet membrane glycoprotein Ibbeta gene affects GPIb/IX complex expression – Bernard-Soulier syndrome in the homozygous form and giant platelets in the heterozygous form. Thromb Haemost. 2001, 86: 1249-1256.PubMedGoogle Scholar
- Kunishima S, Lopez JA, Kobayashi S, Imai N, Kamiya T, Saito H, Naoe T: Missense mutations of the glycoprotein (GP) Ib beta gene impairing the GPIb alpha/beta disulfide linkage in a family with giant platelet disorder. Blood. 1997, 89: 2404-2412.PubMedGoogle Scholar
- Tang J, Stern-Nezer S, Liu PC, Matyakhina L, Riordan M, Luban NL, Steinbach PJ, Kaler SG: Mutation in the leucine-rich repeat C-flanking region of platelet glycoprotein Ib beta impairs assembly of von Willebrand factor receptor. Thromb Haemost. 2004, 92: 75-88.PubMedGoogle Scholar
- Kunishima S, Yamazaki T, Matsushita T, Sako M, Hamaguchi M, Saito H: Variant Bernard-Soulier syndrome caused by compound heterozygous mutations in the GPIb beta gene [abstract]. Platelets. 2004, 15: 374-375.Google Scholar
- de la Salle C, Lanza F, Caen J, Nurden P, Nurden AT, Juhan I, Briquel ME, Ternisien C, Conard J, Cazenave JP: Molecular characterization of five different mutations in patients with Bernard-Soulier bleeding disorder. Central role of platelet GPIbb and GPIX in GPIb-IX-V expression [abstract]. Thromb Haemost. 1997, 68-SupplementGoogle Scholar
- Moran N, Morateck PA, Deering A, Ryan M, Montgomery RR, Fitzgerald DJ, Kenny D: Surface expression of GPIb alpha is dependent on GPIb beta: evidence from a novel mutation causing Bernard-Soulier syndrome. Blood. 2000, 96: 532-539.PubMedGoogle Scholar
- Kunishima S, Matsushita T, Ito T, Kamiya T, Saito H: Novel nonsense mutation in the platelet glycoprotein Ibbeta gene associated with Bernard-Soulier syndrome. Am J Hematol. 2002, 71: 279-284. 10.1002/ajh.10230.View ArticlePubMedGoogle Scholar
- Kenny D, Morateck PA, Gill JC, Montgomery RR: The critical interaction of glycoprotein (GP) IBbeta with GPIX-a genetic cause of Bernard-Soulier syndrome. Blood. 1999, 93: 2968-2975.PubMedGoogle Scholar
- Watanabe R, Ishibashi T, Saitoh Y, Shichishima T, Maruyama Y, Enomoto Y, Handa M, Oda A, Ambo H, Murata M, Ikeda Y: Bernard-soulier syndrome with a homozygous 13 base pair deletion in the signal peptide-coding region of the platelet glycoprotein Ib(beta) gene. Blood Coagul Fibrinolysis. 2003, 14: 387-394. 10.1097/00001721-200306000-00010.View ArticlePubMedGoogle Scholar
- Strassel C, Alessi MC, Juhan-Vague I, Cazenave JP, Lanza F: A 13 base pair deletion in the GPIbbeta gene in a second unrelated Bernard-Soulier family due to slipped mispairing between direct repeats. J Thromb Haemost. 2004, 2: 1663-1665. 10.1111/j.1538-7836.2004.00895.x.View ArticlePubMedGoogle Scholar
- Strassel C, David T, Eckly A, Baas MJ, Moog S, Ravanat C, Trzeciak MC, Vinciguerra C, Cazenave JP, Gachet C, Lanza F: Synthesis of GPIb beta with novel transmembrane and cytoplasmic sequences in a Bernard-Soulier patient resulting in GPIb-defective signaling in CHO cells. J Thromb Haemost. 2006, 4: 217-228. 10.1111/j.1538-7836.2005.01654.x.View ArticlePubMedGoogle Scholar
- Ludlow LB, Schick BP, Budarf ML, Driscoll DA, Zackai EH, Cohen A, Konkle BA: Identification of a mutation in a GATA binding site of the platelet glycoprotein Ibbeta promoter resulting in the Bernard-Soulier syndrome. J Biol Chem. 1996, 271: 22076-22080. 10.1074/jbc.271.36.22076.View ArticlePubMedGoogle Scholar
- Lanza F, de la Salle C, Baas MJ, Schwartz A, Boval B, Cazenave JP, Caen JP: A Leu7Pro mutation in the signal peptide of platelet glycoprotein (GP)IX in a case of Bernard-Soulier syndrome abolishes surface expression of the GPIb-V-IX complex. Br J Haematol. 2002, 118: 260-266. 10.1046/j.1365-2141.2002.03544.x.View ArticlePubMedGoogle Scholar
- Rivera CE, Villagra J, Riordan M, Williams S, Lindstrom KJ, Rick ME: Identification of a new mutation in platelet glycoprotein IX (GPIX) in a patient with Bernard-Soulier syndrome. Br J Haematol. 2001, 112: 105-108. 10.1046/j.1365-2141.2001.02529.x.View ArticlePubMedGoogle Scholar
- Wright SD, Michaelides K, Johnson DJ, West NC, Tuddenham EG: Double heterozygosity for mutations in the platelet glycoprotein IX gene in three siblings with Bernard-Soulier syndrome. Blood. 1993, 81: 2339-2347.PubMedGoogle Scholar
- Noris P, Arbustini E, Spedini P, Belletti S, Balduini CL: A new variant of Bernard-Soulier syndrome characterized by dysfunctional glycoprotein (GP) Ib and severely reduced amounts of GPIX and GPV. Br J Haematol. 1998, 103: 1004-1013. 10.1046/j.1365-2141.1998.01100.x.View ArticlePubMedGoogle Scholar
- Clemetson JM, Kyrle PA, Brenner B, Clemetson KJ: Variant Bernard-Soulier syndrome associated with a homozygous mutation in the leucine-rich domain of glycoprotein IX. Blood. 1994, 84: 1124-1131.PubMedGoogle Scholar
- Donner M, Karpman D, Kristoffersson AC, Winqvist I, Holmberg L: Recurrent mutation Asn45-->Ser of glycoprotein IX in Bernard-Soulier syndrome. Eur J Haematol. 1996, 57: 178-179.View ArticlePubMedGoogle Scholar
- Koskela S, Javela K, Jouppila J, Juvonen E, Nyblom O, Partanen J, Kekomaki R: Variant Bernard-Soulier syndrome due to homozygous Asn45Ser mutation in the platelet glycoprotein (GP) IX in seven patients of five unrelated Finnish families. Eur J Haematol. 1999, 62: 256-264.View ArticlePubMedGoogle Scholar
- Vanhoorelbeke K, Schlammadinger A, Delville JP, Handsaeme J, Vandecasteele G, Vauterin S, Pradier O, Wijns W, Deckmyn H: Occurrence of the Asn45Ser mutation in the GPIX gene in a Belgian patient with Bernard Soulier syndrome. Platelets. 2001, 12: 114-120. 10.1080/09537100020047101.View ArticlePubMedGoogle Scholar
- Sachs UJ, Kroll H, Matzdorff AC, Berghofer H, Lopez JA, Santoso S: Bernard-Soulier syndrome due to the homozygous Asn-45Ser mutation in GPIX: an unexpected, frequent finding in Germany. Br J Haematol. 2003, 123: 127-131. 10.1046/j.1365-2141.2003.04554.x.View ArticlePubMedGoogle Scholar
- Drouin J, Carson NL, Laneuville O: Compound heterozygosity for a novel nine-nucleotide deletion and the Asn45Ser missense mutation in the glycoprotein IX gene in a patient with Bernard-Soulier syndrome. Am J Hematol. 2005, 78: 41-48. 10.1002/ajh.20236.View ArticlePubMedGoogle Scholar
- Suzuki K, Hayashi T, Yahagi A, Akiba J, Tajima K, Satoh S, Sasaki H: Novel point mutation in the leucine-rich motif of the platelet glycoprotein IX associated with Bernard-Soulier syndrome. Br J Haematol. 1997, 99: 794-800. 10.1046/j.1365-2141.1997.4753275.x.View ArticlePubMedGoogle Scholar
- Suzuki K, Hayashi T, Akiba J, Satoh S, Kato T: Phenotypic consequence of the gene abnormality in the platelet glycoprotein IX gene observed in a patient with Bernard-Soulier syndrome through mammalian cell expression system. Thromb Res. 1999, 95: 295-302. 10.1016/S0049-3848(99)00047-X.View ArticlePubMedGoogle Scholar
- Kunishima S, Tomiyama Y, Honda S, Kurata Y, Kamiya T, Ozawa K, Saito H: Cys97-->Tyr mutation in the glycoprotein IX gene associated with Bernard-Soulier syndrome. Br J Haematol. 1999, 107: 539-545. 10.1046/j.1365-2141.1999.01733.x.View ArticlePubMedGoogle Scholar
- Wang Z, Zhao X, Duan W, Fu J, Lu M, Wang G, Bai X, Ruan C: A novel mutation in the transmembrane region of glycoprotein IX associated with Bernard-Soulier syndrome. Thromb Haemost. 2004, 92: 606-613.PubMedGoogle Scholar
- Garner C, Best S, Menzel S, Rooks H, Spector TD, Thein SL: Two candidate genes for low platelet count identified in an Asian Indian kindred by genome-wide linkage analysis: glycoprotein IX and thrombopoietin. Eur J Hum Genet. 2006, 14: 101-108.PubMedGoogle Scholar
- Iwanaga M, Kunishima S, Ikeda S, Tomonaga M, Naoe T: Vulnerable mutation Trp126-->stop of glycoprotein IX in Japanese Bernard-Soulier syndrome. Eur J Haematol. 1998, 60: 264-246.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.