Fig. 4

GBA1 mutation triggers Inflammasome activation in PBMCs of patients with GD. A NLRP3 and C ASC expression. B and D Quantification of the MFI of PBMCs of patients with GD compared with age-, sex-, and BMI-matched HDs (n = 6/group). Representative confocal microscopy imaging of E NLRP3 and G ASC in PBMCs of patients with GD compared with age-, sex-, and BMI-matched HDs. Green indicates NLRP3 or ASC, and blue indicates nuclei. Scale bar: 10 μm. Quantification of MFI of F NLRP3 and H ASC in PBMCs of patients with GD compared with age-, sex-, and BMI-matched HDs. Data are shown as mean ± SEM. P values were determined by two-tailed paired Wilcoxon test (B and D). *P < 0.05, **P < 0.01, and ***P < 0.001