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Fig. 4 | Orphanet Journal of Rare Diseases

Fig. 4

From: Dysregulation of extracellular matrix and Lysyl Oxidase in Ehlers-Danlos syndrome type IV skin fibroblasts

Fig. 4

LOX Activity Assay. Conditioned media from primary human fibrobasts derived from normal (WT) or vEDS individuals was collected and tested for LOX activity. The assay uses a LOX substrate that releases hydrogen peroxide upon transformation by the LOX present in the sample. Hydrogen peroxide is then detected over a 30 min period using a red fluorescence substrate for HRP-coupled reactions and measured at Ex/Em = 540/590 nm using a microplate reader (Molecular Devices iD3)

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Orphanet Journal of Rare Diseases

ISSN: 1750-1172