Targeted next-generation sequencing for detection of PIK3CA mutations in archival tissues from patients with Klippel–Trenaunay syndrome in an Asian population

Table 3 Immunohistochemical analysis of the lymphatic endothelial marker D2-40 and the PI3K/AKT/mTOR signaling pathway

		Anastomosing vascular channels			Small vessels			Venules			Adipose tissues
Patient	D2-40	p-AKT	p-mTOR	p-4EBP1	p-AKT	p-mTOR	p-4EBP1	p-AKT	p-mTOR	p-4EBP1	p-AKT	p-mTOR	p-4EBP1
1	+	+	+	+	+	−	+	+	+	+	+	+	+
2	+	+	−	+	+	−	+	+	+	+	+	−	+
3	−	+	+	+	+	−	+	+	+	+	+	−	+
4	+	+	−	+	+	−	+	+	+	+	+	−	+
5	+	+	−	+	+	−	+	+	−	+	−	−	+
6	+	+	+	+	None	None	None	+	−	+	+	−	−
7	−	+	+	+	+	+	+	+	−	+	−	−	−
8	−	+	+	+	+	−	+	+	+	+	−	−	+
9	−	+	+	+	+	−	+	+	+	+	−	−	+
10	−	+	+	+	None	None	None	+	+	+	+	−	+
11	−	+	−	+	+	−	+	+	+	+	+	−	+
12	+	−	−	+	−	−	+	+	+	+	−	−	+
13	−	−	−	+	+	−	+	+	+	+	−	−	+
14	−	+	−	+	None	None	None	+	−	+	+	−	+

The expression of D2-40 in lymphatic endothelial cells was graded as positive (+) or negative (−). The cytoplasmic intensity of the immunohistochemical stains was graded as positive (+) or negative (−) in p-AKT, p-mTOR, and p-4EBP1. None, no small vessels observed

ISSN: 1750-1172