Fig. 3

Histology and immunohistochemical analysis of the PI3K/AKT/mTOR signaling pathway in the serial sections of patients with Klippel–Trenaunay syndrome. Representative images of anastomosing vascular channels (a-d), small vessels (0.1–1.0 mm diameter) (e-h), venules (10–100 μm diameter) (i-l), and adipose tissues (m-p). Staining for hematoxylin and eosin (HE) (a, e, i, m), p-AKT (b, f, j, n), p-mTOR (c, g, k, o), and p-4EBP1 (d, h, l, p). Cytoplasmic intensity of the immunohistochemical stains graded as positive (b, d, f, h, j, k, l, n, p) and negative (c, g, o). Scale bars: a-d, m-p = 250 μm, e-h = 100 μm, i-l = 50 μm