Fig. 1

(A) In vitro effect on NMDA receptor activity by variant. *Fold effect calculated only when the 95% confidence intervals (CIs) of experimental datasets did not overlap. Positive values indicate a gain of function effect on NMDA receptor activity for the parameter measured. NE = no effect. ^A % remaining current measured at pH 6.8 compared to pH 7.6 at maximal L-glutamate and glycine activation; ^B % residual current in maximum zinc. Results from n = 10–12 oocytes each. (B) A 7 fold reduction in the inhibition sensitivity to endogenous inhibitor Mg⁺⁺ (based on IC₅₀ potency) was evident. (C) Dose response (spike density, measured as discrete events > 0.0 mV) to escalating doses of intrathecal MgSO₄ given each hour. Pre-treatment baseline/washout is depicted as gray bar, treatment epochs in white. (D) Dose response assessing EEG amplitude pre- and post-treatment; pre-treatment baseline/washout is depicted as gray bar, treatment epochs in white. (E) EEG complexity plotted vs. MgSO₄ dose; pre-treatment baseline/washout is depicted as gray bar, treatment epochs in white. Error bars indicate standard error