Fig. 5
From: Identification of a novel de novo mutation of SETBP1 and new findings of SETBP1 in tumorgenesis
SiSETBP1 was able to inhibit the invasion and proliferation of HMC3 and increase the apoptosis of HMC3. And in the mice brains the expression peak of SETBP1 occurred in about E15. A qRT-PCR analysis of SETBP1 in the mice brains taken from embryonic day 11 (E11), E15, postnatal day 1 (P1), P3, P5 and P7. Two adjacent groups were compared. B WB was used to detect the SETBP1 expression of HMC3 cell line with SETBP1 knockdown. GAPDH was used as an internal reference. U87 was used as positive control. C qRT-PCR analysis of SETBP1 of HMC3 cells with SETBP1 knockdown. D Representative photomicrographs of the transwell migration. E Representative photomicrographs of the wound healing assay were taken over time at 0 and 24 h. Black dashed lines delineate the wound area. F HMC2 Cell apoptosis were analyzed by flow cytometry assay 24 h after treatment of siSETBP1#3. Ctrl = control, siNC = small interfering normal control. *P < 0.05, **P < 0.01, ***P < 0.001