Fig. 8

Effect of α-LA treatment on lipid peroxidation. Control and PKAN fibroblasts (P1, P2 and P3) were treated with α-LA at 10 μM for 20 days. a Representative images of lipid peroxidation in treated and untreated control and PKAN cells using BODIPY® 581/591 C11 staining. Control cells treated with Luperox® (500 μM) for 15 min were used as a positive control of mitochondrial lipid peroxidation. Scale bar = 15 μm. b Fluorescence quantification of oxidized form of BODIPY® C11. Data represent the mean ± SD of three separate experiments (50 cell images for each condition). ***p < 0.001, ****p < 0.0001 between PKAN patients and controls. ^#p < 0.05, ^###p < 0.001, between untreated and treated fibroblasts. A.U., arbitrary units