Fig. 3

Gene expression profiles of FOP- and resFOP-ML before and after stimulation. A, Principle component analysis. FOP- and resFOP-MLs were treated with LPS (10 ng/mL) or Activin-A (100 ng/mL) for 24 h and RNAs were extracted at each time point from three biologically independent experiments and processed for microarray analysis. Green and blue circles enclose samples treated with LPS and Activin-A, respectively. Green and blue arrows indicate the migration from the control sample (without treatment). B–E, Volcano plots and the lists of upstream regulators. The expression level of each gene was compared between FOP-ML and resFOP-ML at baseline (B) and after stimulation with Activin-A for 12 h (D). Representative up-regulated (red) and down-regulated (blue) genes are shown (cutoff: fold change greater than 1.2; p value less than 0.05). The list of upstream regulators identified by IPA at each comparison are shown with Z-score (C and E). F–G, Time course analysis of mRNA expression after stimulation with LPS or Activin-A. RNAs were extracted at each time point and assessed for the expression of IIL1B (F), and CCL7(G) genes by quantitative reverse transcriptase PCR (qPCR). The expression levels were normalized to those of resFOP-ML before treatment. The results were obtained in four biologically independent experiments. Tukey–Kramer test *p < 0.05, ***p < 0.001