Fig. 2

Initial protein expression characterization of mutant GFM1 cells. a Western blot analysis of the mutant protein (EF-G1) and several nuclear (UQCRC1, ATP5F1A) and mitochondrial-encoded (mt-ND1, mt-CO2) proteins forming mitochondrial complexes. VDAC was used as mitochondrial mass marker. Galactose samples were taken after 36 h of medium exposition. A representative actin band is shown, although loading control was additionally checked for every Western blot. b Band densitometry of the western blot. Data represents the mean ± SD of 3 independent experiments. *p < 0.05 between control and mutant GFM1 cells; ^$p < 0.05 between glucose and galactose medium control cells. KDa=Kilodalton, kDa