Fig. 4

Overexpression of RRM2 reversed the inhibitory effect of sh-HOTAIR on RB cell proliferation. After treating Y79 and HXO-RB44 cells with the combination of sh-HOTAIR and oe-RRM2, A RRM2 level was measured by Western blot. B Cell cycle was examined by flow cytometry. C Cell proliferative ability was assessed by CCK-8 assay. D Cell apoptosis was assessed by flow cytometry. E Expressions of Ki67, Cleaved Caspase-3, and Bcl-2 were determined by Western blot. Cell experiment was repeated 3 times independently. The results were presented as mean ± standard deviation. Data in panel A were analyzed using the one-way ANOVA, and data in panels B–E were analyzed using the t test, followed by Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001. The relative expressions of all proteins were normalized to the internal reference GAPDH