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Table 2 Primers used to generate SLC25A19 constructs and specified variation sites

From: Identification and functional analysis of novel SLC25A19 variants causing thiamine metabolism dysfunction syndrome 4

Primers Sequences
SLC25A19 5′-cgcggatccatggttggctatgacc-3′
5′-cccaagctttcagcgctggctggct-3′
SLC25A19 c.169G>A p.A57T 5′-cgcagtgaccccagcacaaagtaccatggcatc-3′
5′-gatgccatggtactttgtgctggggtcactgcg-3′
SLC25A19 c.383C>T p.A128V 5′-gtatgtggtggcctggttgcctgtatggccac-3′
5′-gtggccatacaggcaaccaggccaccacatac-3′
SLC25A19 c.76G>A G26R 5′-gctgggtctgtgtctagacttgttactcggg-3′
5′-cccgagtaacaagtctagacacagacccagc-3′
SLC25A19 c.745T>A F249I 5′-ctacaggttggagggattgagcatgccagag-3′
5′-ctctggcatgctcaatccctccaacctgtag-3′
SLC25A19 c.530G>C p.G177A 5′-aggttttctacaaagccttggctcccacctt-3′
5′-aaggtgggagccaaggctttgtagaaaacct-3′
SLC25A19 I33A 5′-gttactcgggcgctggccagtcccttcgacgt-3′
5′-acgtcgaagggactggccagcgcccgagtaac-3′
  1. Mutated sites of each primer are bolded