Table 3 Sequence of primers and probes used for digital polymerase chain reaction (ddPCR)
From: A somatic mutation in PIK3CD unravels a novel candidate gene for lymphatic malformation
Primer and probes | Sequence (5′-3′) |
|---|---|
PIK3CA-F | GCTCAAAGCAATTTCTACACGA |
PIK3CA-R | CTTACCTGTGACTCCATAGAAAATC |
PIK3CA-P-G | 6-FAM- TGAAATCACTGAGCAGGA- BHQ-X |
PIK3CA-P-A | HEX- TGAAATCACTAAGCAGG- BHQ-X |
PIK3CD-F | TCCGAGATGCACGTGCC |
PIK3CD-R | CCTTCATGTGGTGGGTGCT |
PIK3CD-P–T | 6-FAM -TTCGGCCTCATCCT- BHQ-X |
PIK3CD-P–C | HEX -TTCGGCCCCATCC- BHQ-X |