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Fig. 2 | Orphanet Journal of Rare Diseases

Fig. 2

From: Detection of NRAS mutation in cell-free DNA biological fluids from patients with kaposiform lymphangiomatosis

Fig. 2

Results of whole-exome sequencing and Sanger sequencing of the patient with KLA (P1). a Integrative Genomics Viewer (IGV) view of NRAS c.182G > A in DNA samples from leukocytes (upper) and tumor mass (lower). c.182G > A allele was detected in 9 of 176 (5%) alleles. IGV shows reverse strands. b Results of Sanger sequencing of pTA cloning vectors. NRAS exon 2 was amplified by PCR in tumor DNA sample as a template. PCR product was subcloned into pTOPO cloning vector and each plasmid was sequenced. Six of sixty clones (10%) had a c.182G > A allele

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Orphanet Journal of Rare Diseases

ISSN: 1750-1172