|
Cell line
|
NADH:quinone oxidoreductase specific activity w/o rotenone (nmol/min/mg Prot)
|
NADH:quinone oxidoreductase specific activity with rotenone (nmol/min/mg Prot)
|
Inhibition (%)
|
|---|
|
NHDF
|
7.7 (±0.5)
|
1.7 (±0.2)
|
78.3%
|
|
79,787
|
2.5 (±0.2)
|
1.4 (±0.1)
|
42.6%
|
|
79,787-T-ScNDI1
|
24.0 (±8.7)
|
20.7 (±8.3)
|
13.7%
|
|
79,787-T-AtNDB4
|
11.8 (±2.4)
|
10.6 (±1.9)
|
10.0%
|
|
79,787-T-ATNDA2
|
5.3 (±0.9)
|
5.3 (±0.9)
|
2.7%
|
- Spectrophotometric assessment of NADH:quinone oxidoreductase specific activity in control cells (NDHF) and NDUFS4 cell line untransfected (79787) and transfected with alternative NADH oxidases (ScNDI1, AtNDA2 and AtNDB4). Values are expressed as mean ± SD
