Fig. 4

Akt activity is increased in HPS2 lung tissues. a Total and phosphorylated Akt levels in whole lung digests from control and HPS2 mice at baseline and at 7 days after bleomycin (left). Ratio of phosphorylated to total Akt levels (right). b Total and phosphorylated Akt levels in control and HPS2-like MLE12 cells at baseline (left). Ratio of phosphorylated to total Akt levels (right). c Total and phosphorylated Akt levels in control and HPS2-like (Ap3b1 deficient) MLE12 cells at 24 h after bleomycin (left). Ratio of phosphorylated to total Akt levels (right) d) MMP-2 and -9 levels in control and HPS2-like (Ap3b1 deficient) MLE12 cells at 24 h after bleomycin. e. Treatment with Akt inhibitor reduces MMP-2 and -9 levels in control and HPS2-like (Ap3b1 deficient) MLE12 cells at 24 h after bleomycin. Immunoblots are representative of at least two different blots and densitometry analyses (bar graphs) are representative n = 5 or more mouse specimens (*p < 0.05, HPS2 vs control). Data are expressed as mean ± SE, and statistical significance was assessed using a Student’s unpaired t test