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Table 4 Thermal shift assay of wildtype and p.Q188R GALT

From: Arginine does not rescue p.Q188R mutation deleterious effect in classic galactosemia

Melting temperature (Tm, °C)

 

Wildtype GALT

p.Q188R GALT

None

55.8 ± 0.2

54.6 ± 0.2

Arginine

54.8 ± 0.1

54.7 ± 0.4

Galactose

58.1 ± 0.4

56.0 ± 0.3

  1. Comparison of temperature at which half the protein was unfolded (melting temperature, Tm) with no added ligand, arginine (10 mM) or galactose (10 mM). Values presented are the mean values of three replicates
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Orphanet Journal of Rare Diseases

ISSN: 1750-1172