Fig. 6
From: Molecular diagnosis of anti-laminin 332 (epiligrin) mucous membrane pemphigoid
Immunoreactivity of IgG anti-laminin 332 autoantibodies with the GnT-III and GnT-V forms of laminin 332. a Schematic representation of GnT-III and GnT-V laminin 332. b Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the GnT-III (lane 2) and GnT-V (lane 3) proteins shows migration of α3 and γ2 chains at around 160 kDa and β3 chain at around 135 kDa. Weight markers are shown in lane 1. c Immunoreactivity of mucous membrane pemphigoid autoantibodies with the GnT-III and GnT-V isoforms of laminin 332. The two isoforms of laminin 332 were electrophoretically separated by 8% SDS-PAGE, transferred to nitrocellulose and immunoblotted with rabbit anti-human laminin 332 polyclonal antibody (lane 1), anti-laminin 332 mucous membrane pemphigoid patients‘sera (lanes 2–4), normal human sera (lane 5) and normal rabbit sera (lane 6). Anti-laminin 332 autoantibodies reacted in a similar manner with the two isoforms of laminin 332 (GnT-III and GnT-V)