Fig. 4

Effect of PMA on gliosis. a Cells were treated with 10 nM PMA and number of GFAP⁺/vimentin⁺ cells and (b) GFAP⁺/Ki67⁺ cells was quantified. FACS analysis revealed a significant reduction of GFAP⁺/vimentin⁺ cells in all NPC1 mutant cell lines after PMA treatment (N = 4–5, n = 11–28). Number of GFAP⁺/Ki67⁺ cells (b) was reduced in all NPC1 mutant cell lines after treatment with PMA (N = 4–5, n = 7–16). Asterisks above bars indicate significance to untreated control and asterisks above lines indicate significances between treated and untreated cells