Fig. 6

Increase ROS production in chemically-induced Gaucher macrophage. a Mitochondrial ROS levels in control and chemically-induced Gaucher macrophages. Results are expressed as the ratio of MitoSOX signal to 10-N-nonyl acridine orange signal in the absence or presence of CoQ (25 μ M) for 72 h. MitoSOX and 10-N-nonyl acridine orange signal were determined by flow cytometry analysis. Data represent the mean ± SD of three separate experiments. ^c p < 0.05 between control and chemically-induced Gaucher macrophages. *p < 0.05 between the presence and the absence of CoQ treatment. b H₂O₂ levels in control and chemically-induced Gaucher macrophages by CMH2-DCFDA staining coupled with flow cytometry analysis. H₂O₂ levels in control and chemically-induced Gaucher macrophages cultured in the absence or presence of CoQ (25 μ M) for 72 h. Data represent the mean ± SD of three separate experiments. ^c p < 0.05 between control and chemically-induced Gaucher macrophages. *p < 0.05 between the presence and the absence of CoQ treatment. c Representative fluorescence images of MitoSox (red) and GlcCer (green) staining