Figure 1

Western blot analysis of overexpressed wild-type and mutant FGE. Lysates of HEK293T cells transiently transfected with a vector encoding C-terminally flagged wt or mutant FGE (25 μg protein) were assessed for FGE expression by Western-blot. Upper panel: FGE. Lower panel: loading control. NT, not transfected; DUP, p.V174-P318dup.