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Figure 3 | Orphanet Journal of Rare Diseases

Figure 3

From: The modifier role of RET-G691S polymorphism in hereditary medullary thyroid carcinoma: functional characterization and expression/penetrance studies

Figure 3

RET -S891A and RET -G691S/S891A stable mutants anchorage-independent growth ability by soft- agar assay. A. Agar colonies in NIH3T3 cells stably expressing either RET-S891A or RET-G691S/S891A. NIH3T3 cells and RET-M918T-expressing cells are included as negative and positive controls. The mean ± SD of duplicate counts is reported. For RET-S891A and RET-G691S/S891A mutants two independent clones were analyzed and the mean between clones is reported. Data from one representative of two independent soft-agar assays are shown. Below representative images of the plates scored for agar colonies number and the corresponding pictures of agar colonies the day before fixing and staining (scale bar 200 um). B. Agar colonies mean diameter determined by Image-Pro Plus 7.0.1 software. Data are presented as mean diameter ± SD for each sample. For RET-S891A and RET-G691S/S891A two independent clones were analyzed and the mean between clones is reported. ***P < 0.0001 statistical significance determined via Student’s t-test. Below representative images of agar colonies used for diameter measurement (LEICA inverted microscope, scale bar 1000 μm).

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Orphanet Journal of Rare Diseases

ISSN: 1750-1172