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Figure 2 | Orphanet Journal of Rare Diseases

Figure 2

From: The modifier role of RET-G691S polymorphism in hereditary medullary thyroid carcinoma: functional characterization and expression/penetrance studies

Figure 2

RET-S891A and RET -G691S/S891A stable mutants migration ability by wound healing assay. A. Western blot analysis of RET protein and associated ERK signaling in NIH3T3 cells stably expressing either RET-S891A or RET-G691S/S891A. RET was detected by RET C20 primary antibody. Tubulin is shown as protein loading control. Two clones expressing different levels of RET were analyzed for both variants. NIH3T3 cells and RET-M918T-expressing cells are included as negative and positive controls, respectively. B. Wound-healing assay in RET stable mutants. The wound closure percentage was quantified each hour for 24 h post-wound. Graphs are mean of two independent experiments performed in duplicate. C. Wound closure percentage at 12 h post-wound. Data are shown as mean ± SD of two independent experiments performed in duplicate. Statistical significance determined by Two-tailed Unpaired t test. *p < 0.05. D. Representative images at 0 h and 12 h post-wound (x10).

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Orphanet Journal of Rare Diseases

ISSN: 1750-1172