Cysteamine (Lynovex®), a novel mucoactive antimicrobial & antibiofilm agent for the treatment of cystic fibrosis

Table 3 Comparison of the Antibacterial Activity of Cysteamine and Tobramycin in Artificial Sputum Medium and under standard CLSI conditions

	Mueller Hinton Broth MIC ₁₀₀(μg/ml)		Artificial Sputum Medium MIC ₁₀₀(μg/ml)
Isolate	Cysteamine	Tobramycin	Cysteamine	Tobramycin
P. aeruginosa PAO1	250	1	500	4
P. aeruginosa DSMZ1128	250	1	250	2
P. aeruginosa DSMZ1299	250	1	250	1
P. aeruginosa ATCC27853	250	1	500	2

Data represents the mean MIC₁₀₀ (μg/ml) of triplicate samples from triplicate experiments. MICs were determined using the broth microdilution procedure from CLSI Approved Standard M07-A9 [18], with artificial sputum medium substituted for MH broth as appropriate. Data represents the mean MIC₁₀₀ (μg/ml) of triplicate samples from triplicate experiments.