Re-expression studies. (A) Schema of the SERPING1 locus indicating the positions of the most proximal CpG islands. CpG island 1 extends over exon 2 and comprises also part of the contiguous introns 1 and 2, while CpG island 2 is located 5740pb upstream exon 1. (B) Global demethylation induced by 5-Aza-2′-deoxycytidine (5’Aza) and Trichostatin A (TSA), upregulates SERPING1 mRNA expression in Huh7 hepatocellular carcinoma cells more effectively than IFN-γ treatment (C) resulting in an increased C1-INH protein expression in Huh7 culture supernatants, as indicated by western blot analyses with both anti-C1-INH mAbs and biotinylated C1s. Relative quantification of SERPING1 expression was performed using equal amounts of mRNA extracted from unstimulated Huh7 cultures as reference.