Figure 2

Staining results for the BMP2-SMADs-RUNX2 pathway in whisker and eye sections from the Abcc6 KO mouse (×10). Negative controls are shown in the first column (A, E, I, M, Q, U, Y). Alizarin Red stainings confirms profound calcification in the connective tissue capsule of the whiskers (B, F, N, R, V and Z) and Bruch’s membrane of the eye (J). Immunohistochemical staining of adjacent slides with antibodies against respectively Bmp2 (C), Runx2 (G, K), pSmad4 (O), pSmad5 (S), pSmad8 (W) and pSmad1/5/8 (Aa), shows positive staining for all, co-localizing with the mineralization foci. Arrowed areas of positive staining have been magnified (×20). Scale bar = 100 μm. n = 5 each.