Possible effects of EXT2 on mesenchymal differentiation - lessons from the zebrafish

Table 1 Sequences of primers used for quantitative PCR

Primer name	Sequence 5′ → 3′	Lineage	Gene structure
adopql qPCR F1	AACCTGGAAGAGATGGCAGA	A
adopql qPCR R1	CAGGAAAGCCTCTTGGTCCT	A
cebpa qPCR F1	CACAACAGCTCCAAGCAAGA	A	#
cebpa qPCR R1	AATCCATGTAGCCGTTCAGG	A	#
cebpb qPCR F1	TGTTCAGCCCGGACTTTATG	A	#
cebpb qPCR R1	AGTCTGGTACGGCAGGTACG	A	#
col1a2 qPCR F2	CTGGCATGAAGGGACACAG	B
col1a2 qPCR R2	GGGGTTCCATTTGATCCAG	B
col10a1 qPCR F2	CCTGTCTGGCTCATACCACA	C
col10a1 qPCR R2	AAGGCCACCAGGAGAAGAAG	C
osteocalcin qPCR F1	TGAGTGCTGCAGAATCTCCTAA	B
osteocalcin qPCR R1	GTCAGGTCTCCAGGTGCAGT	B
osteopontin qPCR F1	TGAAACAGATGAGAAGGAAGAGG	B
osteopontin qPCR R1	GGGTAGCCCAAACTGTCTCC	B
osterix qPCR F2	GCGTCGATTCTGGAGGAG	B
osterix qPCR R2	AATCTCGGACTGGACTGGTG	B
pparg qPCR F1	GGTTTCATTACGGCGTTCAC	A
pparg qPCR R1	TGCGGCTCTTCTTGTGTATG	A
runx2a qPCR F1	AACTTTCTGTGCTCGGTGCT	B, C
runx2a qPCR R1	GTCATTTCCAGCCATTACCG	B, C
runx2b qPCR F2	CAAACACCCAGACCCTCACT	B, C
runx2b qPCR R2	GTATGACCATGGTGGGGAAG	B, C
scd1 qPCR F1	GTGGCGAAATGTCATTCTGA	A
scd1 qPCR R1	CCATACACGAAACACGCAAA	A
slc25a5 qPCR F1	CCCCCATTGAGAGAGTCAAA	HK
slc25a5 qPCR R1	CCTCTCCAGAACGACAGGAA	HK
sox9a qPCR F1	GGAGCTCAGCAAAACTCTGG	C
sox9a qPCR R1	AGTCGGGGTGATCTTTCTTG	C
srebp1c qPCR F1	CCCCCAGCAGACTCTCTACA	A
srebp1c qPCR R1	CGACAGACTCTGGATCGTCA	A

A, adipocyte-specific gene; B, bone specific gene; C, cartilage specific gene; HK, house keeping gene. #, gene with one exon.
Unless stated otherwise, the primers were designed to construct amplicons of 100–150 bp, spanning at least one intron to avoid amplification from possible genomic DNA contaminant in the template.

ISSN: 1750-1172