Figure 3

14q32 miRNA expression level, metastasis and outcome in human osteosarcoma. (A) miR-382 expression levels determined by qRT-PCR in osteosarcoma primary tumor samples with clinical follow-up information. The miRNA expression levels were normalized relative to normal bone tissues. Osteosarcoma samples were ranked based on expression levels of miR-382 from highest to lowest. Primary osteosarcoma patient samples that later developed metastases are highlighted in red and samples from patients who died due to disease are shown in black. Samples were grouped based on whether they were above or below the median expression level. (B) Kaplan-Meier analysis of metastasis in osteosarcoma patients based on miR-382 expression. Group 1 and Group 2 are significantly different (p-value 0.01). Patients with lowest levels of miR-382 expression showed increased likelihood of metastasis. (C) Kaplan-Meier analysis of survival in osteosarcoma patients based on miR-382 expression. Group 1 and Group 2 are different (p-value 0.08). Patients with lowest levels of miR-382 expression showed decreased likelihood of survival.