Table 2 Primer sets used for AASS mutation analysis

A

[-21M13]-cgattggcagatgagaaggt

1

(c.–221-c.–16)

[M13-Rev]-atctccaccgcatctcacag

B

[-21M13]-cacttgacatcccagttttcc

2

(c.–15-c.210)

[M13-Rev]-ttcctcagctggagtaagca

C

[-21M13]-tgttgtgcctttgctacaca

3

(c.211-c.387)

[M13-Rev]-tcccatctgaaaaacaaggtag

D

[-21M13]-ttgctacctggcgttttctaa

4

(c.388-c.472)

[M13-Rev]-cttgccgcagaaaagagaaa

E

[-21M13]-catgcagattggagaacgag

5 & 6

(c.473-c.687)

[M13-Rev]-atggctgcccacatcatt

F

[-21M13]-ggaaggcaagtggagctatg

7 & 8

(c.688-c.894)

[M13-Rev]-tgggcacatgtagacctgaa

G

[-21M13]-tttcttcggcatgcaataca

9

(c.895-c.1043)

[M13-Rev]-ctgccaagaggtcaagaaaga

H

[-21M13]-gcagagtcctgaagaatgagc

10 & 11

(c.1044-c.1278)

[M13-Rev]-ccccaagagacaagtaagcag

Internal rev seq primer cagcaacccatctcacat

I

[-21M13]-gggcagagttgattgcttgt

12 & 13

(c.1279-c.1406)

[M13-Rev]-gccagccacttagtttggat

J

[-21M13]-ttgtggaatgcaagattctg

14 & 15

(c.1407-c.1655)

[M13-Rev]-tgatttgtgcaccttctgga

Internal rev seq primer cagaaacaaagtagtcttc

K

[-21M13]-gagtgcctgtgtctttttgg

16 & 17

(c.1656-c.1875)

[M13-Rev]-gaacctgggagatggaggtt

Internal forw seq primer ctgagtggatccatggcattg

L

[-21M13]-tcaaatggtacatgctttgaaga

18

(c.1876-c.2016)

[M13-Rev]-gggtttgggatcagggagta

M

[-21M13]-ttctgttgctttctttgttcg

19

(c.2017-c.2184)

[M13-Rev]-caatcaatcataagattcctgaaaaa

N

[-21M13]-gacaggaaaacctgctaggc

20

(c.2185-c.2280)

[M13-Rev]-gactcccatcactgggtcac

O

[-21M13]-ttgaggtgtatttgaagttcagtg

21 & 22

(c.2281-c.2485)

[M13-Rev]-acatcttcccattcctgctg

Internal rev seq primer ctacccacattagagcaacg

P

[-21M13]-ggcaggaggagatgacagac

23

(c.2486-c.2662)

[M13-Rev]-actcagccaccttggaactg

Q

[-21M13]-aaaatgcctaggcctccaag

24

(c.2663-c.*187)

[M13-Rev]-gtggcttgcatctcctgttc

R

5^′-tctccaagggtcttcaccac-3^′

c.47-c.66

5^′-agaatgccaccagctttgac-3^′

c.236-c.217

S

5^′-agttcctcaggcagagtcca-3^′

c.2421-c.2440

5^′-ggctgaaaagccattgatgt-3^′

c.2607-c.2588

T

5^′-gccttccatcccagtttctt-3^′

c.–116-c.–97

5^′-ctgcatctctcaccacagga-3^′

c.1323-c.1342

Internal seq. primer 5^′-tgcatttttctcccacacaa-3^′

c.318-c.337

Internal seq. primer 5^′-cccaaactggagacctcaga-3^′

c.755-c.774

U

5^′-gaatgctttggagacatgctt-3^′

c.1237-c.1257

5^′-ggtgtattgcctgggaagaa-3^′

c.*23-c.*42

Internal seq. primer 5^′-ctgcaagctacatcacaccag-3^′

c.1724-c.1744

Internal seq. primer 5^′-tggcatttcttctgctcaca-3^′

c.2136-c.2155