Figure 1
From: A mutation in the c-Fos gene associated with congenital generalized lipodystrophy
Localization of the postreceptor defect to c-fos expression. A) Western blot analyses of Akt phosphorylation and abundance. B) Phosphorylation of MAPK was assayed by western blot analyses. Activity of MAPK was detected in in-gel kinase assays. C) Formation of the ternary complex at sre element of c-fos promoter in control and patient. The specific complex is indicated by an arrow (lane F: free probe, 1: no competition; competition: 2: 100x SRE, 3: 10x SRE, 4: 100x SP-1, 5: 10x SP-1). D) Activation of ternary complex factor Elk-1 following insulin and PDGF stimulation in patient and control cells. Results are given as means (±S.D.). *p< 0.05 vs basal control. E) Transcriptional activation of c-fos mRNA. The mRNA levels were normalized against 18S rRNA as internal control. Values are means (±S.D.) from four independent experiments, each performed in triplicate. *p< 0.05 vs basal control.