Figure 4

Amlexanox treatment leads to the synthesis of truncated and/or full length proteins from nonsense mutation-containing mRNAs. Calu-6 (A), DMD (B) or 6CFSMEo- (C) cells were incubated with amlexanox molecule or with DMSO (−) as a control before purifying proteins and performing Western blot to detect p53, dystrophin or CFTR protein, respectively, using antibodies raised against N-terminal part of the proteins (and C-terminal part of the dystrophin protein as mentioned). The three left lanes represent a two-fold serial dilution of cell extract from untreated cells (Calu-3 cells for (A) and (C) or WT myoblasts for (B)). Each gel is representative of at least 2 independent experiments. On the left of each gel is indicated the molecular weight ladder (BenchMark pre-stained for A and C, or HiMark pre-stained for B (Life Technologies)).