Figure 1

Identification of amlexanox as putative NMD inhibitor. Luciferase activity was measured from cells expressing the Firefly luciferase with MS2 binding sites in its 3’UTR and MS2-UPF1 (up and left), MS2-UPF2 (up and right), MS2-UPF3 (down and left) or MS2-UPF3X (down and right). Tested drugs were added in columns 2 to 11 of each 96-well plate, the vehicle (DMSO) was added in positions A1, B1 and C1. No compounds were added in the rest of column 1 and column 12 of each plate, as negative controls. The luciferase activity is provided in arbitrary units. The mean luminescence values were calculated from all test compounds and for each plate. The threshold of hit selection was calculated for each plate and is represented by the black thick line: m + 3σ (mean plus 3 standard deviations).