Figure 3
From: Novel genetic linkage of rat Sp6 mutation to Amelogenesis imperfecta
Analyses of longitudinal sections of the maxillary incisors of 6-week-old rats. A. Immunohistochemical analysis. Incisor sections were immunostained with antiserum against rat Wt-SP6. The sections were prepared from rats heterozygous (Wt/Ami) or homozygous (Ami/Ami) for mutant Sp6. Animals were sorted based on the transgenic Sp6 (Tg) genotype. Scale bar, 100 mm. Ab, ameloblasts; Em, enamel matrix; Es, enamel space; Ob, odontoblasts. B. The length of the ameloblast layer for the indicated differentiation stages. Columns represent the average of two to three independent samples; bars indicate standard deviation. Statistical significance was evaluated by unpaired t tests for an indicated set of data. *p < 0.05, **p < 0.01.