Development of an ELISA for sensitive and specific detection of IgA autoantibodies against BP180 in pemphigoid diseases

Table 2 Immunoreactivity profile of linear IgA disease patients

	Positive indirect immunofluorescence	Immunoblot with native shed BP180 ectodomain		Immunoblot with recombinant BP180 ectodomain		ELISA BP230-IgA		ELISA BP230-IgG
		*positive*	*negative*	*positive*	*negative*	*positive*	*negative*	*positive*	*negative*
ELISA BP180 positive	25 (83%)	20(77%)	3 (11.5%)	20 (71.4%)	4 (14.3%)	4 (13.3%)	21 (70%)	0 (0%)	25 (83%)
ELISA BP180 negative	5 (17%)	1 (3.8%)	2 (7.7%)	1 (3.6%)	3 (10.7%)	1 (3.3%)	4 (13.3%)	0 (0%)	5 (17%)
	n = 30		n = 26		n = 28		n = 30		n = 30

ISSN: 1750-1172