Figure 1

Recombinant ectodomain of BP180 used in this study. A: Schematic representation of human BP180 and its ectodomain consisting of alternating collagenous (C) and non-collagenous (NC) domains. The recombinant form of BP180 ectodomain used for the development of the current ELISA system spans from aa 490 through aa 1497 and has an NH₂-terminal hexahistidine tag. B: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified recombinant protein without and with previous boiling, which migrates around 360 (lane 2) and 120 kDa (lane 3), respectively. C: Immunoblot analysis of the native, normal human keratinocyte-derived shed BP180 ectodomain (lane 1) and the recombinant BP180 ectodomain (lane 2) using a monoclonal antibody specific to BP180. Weight markers of 116 and 66 kDa are shown to the left.