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Table 4 Mean PCR amplification efficiencies (n = 4)

From: Allele-specific differences in ryanodine receptor 1 mRNA expression levels may contribute to phenotypic variability in malignant hyperthermia

Muscle sample #

Amplification efficiencies and coefficient of correlation (E/R2)

 

AS1

AS2

HPRT

1

1.921/0.9993

1.941/0.9993

1.956/0.9987

2

2.068/0.9989

2.104/0.9995

1.999/0.9995

3

2.030/0.9989

2.045/0.9996

2.028/0.9988

4

2.011/0.9986

2.079/0.9993

1.976/0.9895

  1. Dilutions series used for the determination of the PCR amplification efficiencies for each of the four MHS muscle tissues: 1; 1:5 dilution steps, 2; 1:3 dilution steps, 3; 1:3 dilution steps, 4; 1:3 dilution steps.
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Orphanet Journal of Rare Diseases

ISSN: 1750-1172