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Figure 1 | Orphanet Journal of Rare Diseases

Figure 1

From: Allele-specific differences in ryanodine receptor 1 mRNA expression levels may contribute to phenotypic variability in malignant hyperthermia

Figure 1

Constructed standard curves for the determination of the PCR amplification efficiencies. Serial diluted 1:5 cDNA dilutions from the #1 MHS muscle biopsy. Each standard curve represents the pooled results of four individual experiments. Amplifications in each individual experiment were conducted in triplicate. Housekeeping gene; HRPT (triangles), wild type RYR 1; AS1 (diamonds) and mutant RYR 1; AS2 (squares). The error bars show the standard deviation. The regression coefficient was greater than 0.99 in all cases.

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Orphanet Journal of Rare Diseases

ISSN: 1750-1172