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  • Open Access

MALDI spectrometry for salivary samples analysis : a new tool for TTR amyloidosis diagnosis

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Orphanet Journal of Rare Diseases201510 (Suppl 1) :P46

  • Published:


  • Amyloidosis
  • Amyloid Deposit
  • Electrospray Mass Spectrometry
  • MALDI Mass Spectroscopy
  • Salivary Sample


Amyloidosis suffers from a lack of accurate diagnosis tools. It results from a wrong folding of specific proteins and their identification is essential for proper medical care. Today most patients cases are identified thanks to immunohistochemistry analysis after surgery or biopsy on the defective tissues. The aim of our study is to show that diagnosis and typing of TTR amyloidosis can be immediately and rapidly achieved on formaline fixed and paraffin embedded biopsy samples using MALDI spectrometry, and also on salivary samples.


Four fresh or formalin fixed and paraffin embedded Myocardial and salivary glands samples were analyzed. A specific de-waxing protocol using trypsic digestion and antigen retrieval was used for paraffin embedded samples. After CHCA matrix deposit, MALDI mass spectromerty acquisition was performed using MALDI-TOF, MALDI-TOF-TOF et MALDI QIT-TOF. bottom approaches using Electrospray mass spectrometry on high resolution orbitrap instruments was also performed on salivary samples.


On tissue samples the m/z ratio peak was 1366.78. Its MS/MS analysis allows to obtain m/z 1348.70, 1192.59, 1045.54, 946.46 ions, in other words, the following 4 ions coming from the 22-34 TTR-peptide GSPAINVAVHFR. On salivary samples the m/z ratio was 15991 matching with transthyretin. After MS/MS fragmentation, m/z 1047.5113 and 1051.5232 ions were identified corresponding respectively to Wild Type TTR and mutated THR 49ILE.


TTR is responsible for amyloid deposits. Formalin fixed and paraffin embedded samples can be ex post analyzed after a specific de-waxing protocol by MALDI mass spectroscopy. Mutated TTR can also be identified on salivary samples. Such an approach has to be evaluated in further studies.

Authors’ Affiliations

Médecine Interne, CHU Timone, 1/2, Marseille, France
INSERM UMR 911, Plateforme protéomique PIT2, 2, Marseille, France
CHU Timone, Service de Cardiologie, 3, Marseille, France
Service de neurologie, CHU Timone, 4, Marseille, France
Service de Médecine Interne Génerale, Hopitaux Universitaires de Genève, 5, Genève, Suisse


© Seguier et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.