Table 1 The primers and fragment length of Full-length amplification of PAH
From: Identification of deep intronic variants of PAH in phenylketonuria using full-length gene sequencing
Fragment | Primers | Primer sequence 5′–3′ | Genomic locus | Fragment length |
|---|---|---|---|---|
1 | PAH-L1F | ACTCTCTTCTCCTCCCTAGTGCG | chr12:103311000 | 11,916Â bp |
PAH-L1R | CCAAATAGCTCCCTGATTCACCC | chr12:103299084 | ||
2 | PAH-L2F | AAGGTAGACAAGGTGGTAGGACT | chr12:103299364 | 3975Â bp |
PAH-L2R | GAGAGCACACTTCAAAAAGAAGG | chr12:103295389 | ||
3 | PAH-L3F | TGCTTTTCCCACTGTTACACTCC | chr12:103295660 | 4029Â bp |
PAH-L3R | GGCTGGATGACTCAAGAGTTTTA | chr12:103291631 | ||
4 | PAH-L4F | ACGAATGTGGGAGTGGGATGCTT | chr12:103291906 | 3144Â bp |
PAH-L4R | ACCAGAACAGGAAAACCTAACGC | chr12:103288762 | ||
5 | PAH-L5F | GTCTGACCCCCTATTCAAGCAGA | chr12:103289098 | 11,693Â bp |
PAH-L5R | ACCTCTGAGCTCTGCACCTTGTC | chr12:103277405 | ||
6 | PAH-L6F | CCCATCAACCCTCTGAAGGACGT | chr12:103277697 | 10,531Â bp |
PAH-L6R | TGAAGGAGGGTACAGCCATTGGT | chr12:103267166 | ||
7 | PAH-L7F | TCGTGAGTGGTAGTTTTCCATGG | chr12:103267491 | 5388Â bp |
PAH-L7R | AAGGAAGGGAGGGAAGATAGGAG | chr12:103262103 | ||
8 | PAH-L8F | TAGAAACGAGGCACAACAGTAGT | chr12:103262383 | 2337Â bp |
PAH-L8R | AGGCTGTTTTATTCAGGACCGAG | chr12:103,260,046 | ||
9 | PAH-L9F | CCTGTGTACCGTGCAAGACGGAA | chr12:103260407 | 8101Â bp |
PAH-L9R | TCTCACCACATTGCACTCATTCC | chr12:103,252,306 | ||
10 | PAH-L10F | CCTCTACACACTGCCTTAAATGT | chr12:103252590 | 4048Â bp |
PAH-L10R | ACACAAACACACACTCCTAACTC | chr12:103248542 | ||
11 | PAH-L11F | GGAAGACAAGTATGTGGAGGCAA | chr12:103,248,822 | 4049Â bp |
PAH-L11R | GATTGTTTGAGCCCAGAAGTCTG | chr12:103244773 | ||
12 | PAH-L12F | CCTTGTCAGGCAGTTATTTGTGT | chr12:103245076 | 3913Â bp |
PAH-L12R | ATAGCTGGAGAGTCTAACACATC | chr12:103,241,163 | ||
13 | PAH-L13F | CCCTGGAGCTCTTAGTCCCTCTTGTTT | chr12:103241472 | 10,642Â bp |
PAH-L13R | AGCAAGATCATCTGTCAGTAAAGACTG | chr12:103230830 |