Fig. 4

Spatial distribution of protein key sites and changes of amino acid interaction force before and after mutation. A, B KIF21A protein structure (cartoon/surface form) and spatial distribution of F355 residue. C, D Interaction force analysis of the KIF21A F355 and KIF21A F355S with the surrounding amino acid residues. E Showing positions of F355 and previously reported variants associated with CFEOM in the motor domain. F, G CHN1 protein structure (cartoon/surface form) and spatial distribution of H217 residue. H, I Interaction force analysis of the CHN1 H217 and CHN1 H217R with the surrounding amino acid residues. J Showing positions of H217 and other nearby previously reported variants in the same domain. K, L TUBB3 protein structure (cartoon/surface form) and spatial distribution of S78 residue. M, N Interaction force analysis of the TUBB3 S78 and TUBB3 S78T with the surrounding amino acid residues. O Showing positions of S78 and other nearby previously reported variants in the same domain